医学临床研究
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医学临床研究  2020, Vol. 37 Issue (3): 378-381    DOI: 10.3969/j.issn.1671-7171.2020.03.017
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莪术醇对小鼠黑色素瘤B16细胞增殖和迁移的影响
宁宁, 蒋宇, 邹联红, 冯浩**
湖南师范大学第一附属医院暨湖南省人民医院,湖南 长沙 410005
Effect of Curcumol on Proliferation and Migration of Mouse Melanoma B16 Cells
NING Ning, JIANG Yu, ZOU Lian-hong, et al
Medical department of Hunan provincial people's hospital, Changsha 410005,Hunan
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摘要 【目的】探讨莪术醇对小鼠黑色素瘤B16细胞增殖和迁移的影响。【方法】10%胎牛血清的RPMI-1640培养基培养小鼠黑色素瘤B16细胞。不同剂量莪术醇(50、100、200 μmol/L)处理B16细胞后,检测细胞增殖、迁移和miR-7-5p含量;miR-7-5p inhibitor预处理后,再用莪术醇100 μmol/L处理B16细胞,检测细胞增殖和迁移。【结果】不同剂量莪术醇(50、100、200μmol/L)处理B16细胞后,细胞490 nm吸光度值均低于对照组,且随着莪术醇浓度的增加,细胞490 nm吸光度值降低显著,差异均有统计学意义(P<0.05)。不同剂量莪术醇(50、100、200μmol/L)处理B16细胞后,穿膜细胞数均低于对照组, 且随着莪术醇浓度的增加,穿膜细胞数降低显著,差异均有统计学意义(P<0.05)。通过real-time PCR验证miR-7-5p inhibitor转染B16细胞后的抑制效果, NC-inhibitor和miR-7-5p inhibitor转染后细胞miR-7-5p表达分别为(1.02±0.10)和(0.41±0.14),差异有统计学意义(P<0.05)。抑制B16细胞miR-7-5p表达后,观察莪术醇对细胞增殖的调控,与NC inhibitor相比(1.413±0.108),miR-7-5p inhibitor升高莪术醇处理B16细胞490nm吸光度值(1.697±0.089),差异有统计学意义(P<0.05)。【结论】莪术醇能够抑制小鼠黑色素瘤B16细胞增殖和迁移,其机制可能与增加miR-7-5p表达有关。
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宁宁
蒋宇
邹联红
冯浩
关键词 黑色素瘤/病理学肿瘤细胞 培养的    
Abstract:【Objective】To investigate the effect and mechanism of curcumol on proliferation and migration of mousemelanoma B16 cell. 【Methods】B16 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum. The proliferation, migration and mir-7-5p content of B16 cells treated with Curcumol (50, 100, 200μmol / L) were detected. B16 cells were pretreated with mir-7-5p inhibitor, then treated with Curcumol (100 μ mol / L) to detect cell proliferation and migration.【Results】The 490 nm absorbance of B16 cells treated with different doses of Curcumol (50, 100, 200μmol / L) was lower than that of the control group, and with the increase of Curcumol Concentration, the 490 nm absorbance of the cells decreased significantly, the difference was statistically significant (P<0.05). After treatment of B16 cells with different doses of Curcumol (50, 100, 200 μ mol / L), the number of transmembrane cells was lower than that of the control group, and with the increase of Curcumol Concentration, the number of transmembrane cells decreased significantly, the difference was statistically significant (P<0.05).The inhibitory effect of miR-7-5p inhibitor on B16 cells was verified by real-time PCR. After NC inhibitor and miR-7-5p inhibitor were transfected, the miR-7-5p of the cells were (1.02±0.10) and (0.41±0.14), respectively, with statistical significance (P<0.05). After inhibiting miR-7-5p of B16 cells, the regulation of Curcumol on cell proliferation was observed, compared with NC inhibitor (1.413) and the difference was statistically significant (P<0.05). Compared with NC inhibitor (1.413±0.108), miR-7-5p inhibitor increased the absorbance value (1.697±0.089) of B16 cells treated with Curcumol,the difference was statistically significant (P<0.05).【Conclusion】Curcumol could inhibit cell proliferation and migration of mouse melanoma B16 cells, and its mechanism might be related to the increased expression of miR-7-5p.
Key wordsMelanoma/PA    Tumor Cells    Cultured
收稿日期: 2019-05-21     
PACS:  R730.261  
基金资助:湖南省科技厅重点研发计划项目(2016SK2044);湖南省卫生计生委科研计划项目(B20180802);湖南省自然科学青年基金项目(2009JJ4011)
通讯作者: **, Email:doctorfenghao@126.com   
引用本文:   
宁宁, 蒋宇, 邹联红, 冯浩. 莪术醇对小鼠黑色素瘤B16细胞增殖和迁移的影响[J]. 医学临床研究, 2020, 37(3): 378-381.
NING Ning, JIANG Yu, ZOU Lian-hong, et al. Effect of Curcumol on Proliferation and Migration of Mouse Melanoma B16 Cells. JOURNAL OF CLINICAL RESEARCH, 2020, 37(3): 378-381.
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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2020.03.017     或     http://journal07.magtech.org.cn/yxlcyj/CN/Y2020/V37/I3/378
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