Abstract：Objective]To analyze the changes of miRNA expression profile in cardiomyocytes of neonate rats after hypoxia preconditioning ,and to explore the mechanism of hypoxia preconditioning and therapeutic target .[Methods]Pri‐mary cardiomyocytes of neonate rats were cultured .Survival rate of rat cardiomyocytes and lactate dehydrogenase(LDH) level were detected after hypoxia preconditioning and hypoxia reoxygenation .The expression profiles of miRNA in normal and hypoxia preconditioned cardiomyocytes were detected by miRNA genetic chip .The results were verified by real‐time quantitative polymerase chain reaction(PCR) ,and the biological function of miRNA significantly differentially expressed were analyzed .[Results]The successful establishment of hypoxia preconditioning models was verified by the results of sur‐vival rate of rat cardiomyocytes and LDH levels .The miRNA chip detection results showed that 6 miRNAs in hypoxia preconditioned cardiomyocytes were up‐regulated and 5 miRNAs were down‐regulated in comparison with normal cardio‐myocytes .The biological function of some miRNAs was related to cardiovascular function .[Conclusion]Hypoxia precon‐ditioning leads to the changes of miRNA expression profile which may serve as potential targets for the treatment of hy‐poxia reoxygenation injury .
2014, Vol. 31 
