Abstract：To construct the antisense nucleic acid eukaryotic expression vector of human cy-clooxygenase-2(COX-2) ,and to explore its role in inhibiting the expression of COX-2 in human bladder cancer T24 cells .[Methods] PCR method was used to amplify cDNA from COX-2 plasmid .The COX-2 cDNA was cloned into the sites between T vectors EcoR 1 and Xba I through restrictive endonuclease ,and then inversely inserted into pcDNA3 .1 to obtain the recombinant plasmid .The recombinant plasmid was transfected into bladder cancer T24 cells mediated by liposome .After the scanning by G418 ,flow cytometry ,indirect immuno-fluorescence staining and RT-PCR were performed .[Results] Nucleic acid and restrictive endonuclease diges-tion confirmed that COX-2-antisense nucleic acid eukaryotic expression plasmid pcDNA 3 .1/COX-2 was cor-rect .Identification showed that the expression of COX-2 in T24 cells transfected with pcDNA3/COX-2 was in-hibited obviously .[Conclusion] Antisense nucleic acid eukaryotic expression vector of COX-2 is constructed successfully .Bladder cancer cell line T24-AS with persistent downregulation of COX-2 expression is obtained .
引用本文:
张军卫%靳风烁. 构建人 COX-2反义核酸真核表达载体对膀胱癌 T24细胞株COX-2表达的作用[J]. 医学临床研究, 2014, 31(8): 1465-1467.
ZHANG Jun-wei%JIN Feng-shuo. Effect of Human Antisense Nucleic Acid of Cyclooxygenase-2 (COX-2) on Expression of COX-2 in Bladder Cancer T24 Cells. JOURNAL OF CLINICAL RESEARCH, 2014, 31(8): 1465-1467.
2014, Vol. 31 
