Abstract：【Objective】 To investigate the effect of XIAP gene silencing on the biological characteristics of ovarian cancer SKOV3 cells. 【Methods】 The human ovarian cancer cell line SKOV3 were transfected with XIAP/siRNA and XIAP/smRNA respectively, and the PBS were used as the control group. The cell transfection efficiency was detected. The apoptosis rate was detected by flow cytometry. The number of cell migration and invasion were detected by Transwell chamber, and the expression of related protein levels were detected by Western blot. 【Results】 Compared with the control group, the level of XIAP protein in XIAP/siRNA group decreased significantly, but no significant change was found in XIAP/smRNA group. The level of apoptosis in XIAP/siRNA group was significantly higher than that in the XIAP/smRNA group and the control group (P<0.05). The number of model penetrating cells and metastatic cells in the XIAP/siRNA group was significantly lower than that in the control group and the XIAP/smRNA group (P<0.05), but there was no significant difference between the control group and the XIAP/smRNA group (P>0.05). The relative expression level of E-cad protein in the XIAP/siRNA group was 0.312 ±0.08, which was significantly lower than that in the control group and the XIAP/smRNA group (1.065 ± 0.01, 1.026 ± 0.2), and the difference was statistically significant (P<0.05), but there was no significant difference between the XIAP/smRNA group and the control group (P>0.05).【Conclusion】 XIAP gene silencing can effectively inhibit apoptosis, metastasis and invasion of ovarian cancer SKOV3 cells, and its mechanism may be related to the regulation of E-cad expression.
杨婷, 杨淑梅. XIAP基因沉默对卵巢癌SKOV3细胞生物学特性的影响[J]. 医学临床研究, 2018, 35(12): 2301-2303.
YANG Ting, YANG Shu-mei. Effect of XIAP Gene Silencing Expression on Biological Characteristics of Ovarian Cancer SKOV3 Cells. JOURNAL OF CLINICAL RESEARCH, 2018, 35(12): 2301-2303.
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2018, Vol. 35 
