XIAP基因沉默对卵巢癌SKOV3细胞生物学特性的影响

doi:10.3969/j.issn.1671-7171.2018.12.004

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摘要 【目的】 探讨XIAP基因沉默对卵巢癌SKOV3细胞生物学特性的影响。【方法】 人卵巢癌细胞株SKOV3分别转染XIAP/siRNA、XIAP/smRNA,以PBS作为对照组。检测细胞转染效率,采用流式细胞仪检测细胞凋亡率,采用Transwell 上室检测细胞转移与侵袭数目,采用Western blot 检测相关蛋白表达水平。【结果】 与对照组相比,XIAP/siRNA组的细胞中XIAP蛋白水平显著下降,XIAP/smRNA组无显著变化。XIAP/siRNA组细胞凋亡水平显著高于XIAP/smRNA组与对照组(P<0.05),穿模细胞数和转移细胞数显著低于对照组和XIAP/smRNA组,其差异有显著性(P<0.05);但对照组和XIAP/smRNA组上述指标比较差异无统计学意义 (P>0.05);XIAP/siRNA组的E-cad蛋白相对表达水平为0.312±0.08,显著低于对照组、XIAP/smRNA组的1.065±0.01、1.026±0.2,其差异有统计学意义(P<0.05),但XIAP/smRNA组与对照组比较差异无显著性(P>0.05)。【结论】 XIAP基因沉默能有效抑制卵巢癌SKOV3细胞的凋亡,抑制细胞转移与侵袭,其作用机制可能与调节E-cad表达有关。

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	杨婷
	杨淑梅

关键词 ：卵巢肿瘤/超微结构, 蛋白质类

Abstract：【Objective】 To investigate the effect of XIAP gene silencing on the biological characteristics of ovarian cancer SKOV3 cells. 【Methods】 The human ovarian cancer cell line SKOV3 were transfected with XIAP/siRNA and XIAP/smRNA respectively, and the PBS were used as the control group. The cell transfection efficiency was detected. The apoptosis rate was detected by flow cytometry. The number of cell migration and invasion were detected by Transwell chamber, and the expression of related protein levels were detected by Western blot. 【Results】 Compared with the control group, the level of XIAP protein in XIAP/siRNA group decreased significantly, but no significant change was found in XIAP/smRNA group. The level of apoptosis in XIAP/siRNA group was significantly higher than that in the XIAP/smRNA group and the control group (P<0.05). The number of model penetrating cells and metastatic cells in the XIAP/siRNA group was significantly lower than that in the control group and the XIAP/smRNA group (P<0.05), but there was no significant difference between the control group and the XIAP/smRNA group (P>0.05). The relative expression level of E-cad protein in the XIAP/siRNA group was 0.312 ±0.08, which was significantly lower than that in the control group and the XIAP/smRNA group (1.065 ± 0.01, 1.026 ± 0.2), and the difference was statistically significant (P<0.05), but there was no significant difference between the XIAP/smRNA group and the control group (P>0.05).【Conclusion】 XIAP gene silencing can effectively inhibit apoptosis, metastasis and invasion of ovarian cancer SKOV3 cells, and its mechanism may be related to the regulation of E-cad expression.

Key words： Ovarian Neoplasms/UL Proteins

收稿日期: 2018-04-08

PACS:

R737.31

基金资助:陕西省教育厅科研项目(编号:2013JK797)

通讯作者: **,E-mail:Yangshumei_1967@163.com

引用本文:

杨婷, 杨淑梅. XIAP基因沉默对卵巢癌SKOV3细胞生物学特性的影响[J]. 医学临床研究, 2018, 35(12): 2301-2303.
YANG Ting, YANG Shu-mei. Effect of XIAP Gene Silencing Expression on Biological Characteristics of Ovarian Cancer SKOV3 Cells. JOURNAL OF CLINICAL RESEARCH, 2018, 35(12): 2301-2303.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2018.12.004 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2018/V35/I12/2301

[1] Miyamoto M, Takano M, Aoyama T,et al. Phenoxodiol increases cisplatin sensitivity in ovarian clear cancer cells through xiap down-regulation and autophagy inhibition[J].Anticancer Res,2018, 38(1):301-306.
[2] 洪澜, 李成学, 贺国丽, 等. 卵巢癌生物治疗的现状与进展[J].现代生物医学进展, 2016, 16(6):1180-1183.
[3] Zhao Y, Tian B, Wang Y, et al. Kaempferol sensitizes human ovarian cancer cells-ovcar-3 and skov-3 to tumor necrosis factor-related apoptosis-inducing ligand (trail)-induced apoptosis via jnk/erk-chop pathway and up-regulation of death receptors 4 and 5[J].Med Sci Monit,2017, 26(23):5096-5105.
[4] Li W, Chen Y, Sheng Y, et al. Synthesis and inhibitory effect of 10-chlorocanthin-6-one on ovarian cancer HO8910PM cells[J].Biotechnol Lett,2018, 40(1):23-30.
[5] 李冬冬, 钟洁, 王莉, 等. NAC-1通过自噬介导卵巢癌细胞顺铂耐药的研究[J].现代妇产科进展, 2018, 27(1):14-17.
[6] Li X. MicroRNA 146a 5p enhances cisplatin induced apoptosis in ovarian cancer cells by targeting multiple anti apoptotic genes[J].Int J Oncol,2017, 51(1):327-335.
[7] Wang Y, Liu P, Wang X, et al. Role of X linked inhibitor of apoptosis associated factor 1 in vasculogenic mimicry in ovarian cancer[J].Mol Med Rep,2017, 16(1):325-330.
[8] 杨波, 李胜泽, 马玲, 等. miR-132靶向调控Ezrin抑制卵巢癌细胞增殖、促进凋亡的实验研究[J].中国免疫学杂志, 2017, 33(1):72-75.
[9] Li S, Yang L, Wang J, et al. Analysis of the chemotherapeutic effects of a propadiene compound on malignant ovarian cancer cells[J].Oncotarget,2016, 7(35):57145-57159.
[10] Chen W. MicroRNA-509-3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis[J].Pharmacogenomics,2016, 17(3):187-197.
[11] 代娟, 韩萍, 曹燕花, 等. MicroRNA-101对上皮性卵巢癌耐药细胞SKOV3/DDP顺铂敏感性的影响[J].中国现代医学杂志, 2017, 27(10):25-29.
[12] Li X, Chen W, Zeng W, et al. microRNA-137 promotes apoptosis in ovarian cancer cells via the regulation of XIAP[J].Br J Cancer,2017, 116(1):66-76.
[13] 顾海风, 黄绮丹, 涂画. 初次肿瘤细胞减灭术中行系统性腹膜后淋巴结清扫对晚期卵巢癌预后的影响[J].实用医学杂志, 2016, 32(10):1623-1627.
[14] 李君, 刘恩令, 杨丽. 雌激素受体与卵巢癌SKOV3细胞顺铂耐药的相关性研究[J].中国临床药理学杂志, 2017, 33(4):347-350.
[15] Chen W, Huang L, Hao C, et al. MicroRNA-155 promotes apoptosis in SKOV3, A2780, and primary cultured ovarian cancer cells[J].Tumour Biol,2016, 37(7):9289-9299.
[16] 毛静月, 刘蕊, 李彩云, 等. siRNA靶向沉默YAP1对卵巢癌细胞skov3增殖凋亡的影响[J].中国老年学杂志, 2017, 37(4):797-799.
[17] Xu B, Lefringhouse J, Liu Z, et al. Inhibition of the integrin/FAK signaling axis and c-Myc synergistically disrupts ovarian cancer malignancy[J].Oncogenesis,2017, 6(1):e295.
[18] Ayyagari VN, Hsieh TJ, Diaz-Sylvester PL, et al. Evaluation of the cytotoxicity of the Bithionol-cisplatin combination in a panel of human ovarian cancer cell lines[J].BMC Cancer,2017, 17(1):49.