miR-484通过调控HIF-1α/VEGF信号通路对胃癌血管生成的影响<sup>*</sup>

doi:10.3969/j.issn.1671-7171.2024.09.003

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摘要【目的】探讨miR-484是否通过调控HIF-1α/VEGF信号通路参与胃癌血管的生成。【方法】分别在阴性对照(miR-NC组)、miR-484 模拟物(miR-484 mimics组)和miR-484 抑制剂(miR-484 inhibitors组)3种条件下转染SCG-7901胃癌细胞。检测不同组SCG-7901细胞中缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)的mRNA和蛋白表达情况及胃癌细胞的增殖情况。将转染成功的SCG-7901细胞与血管内皮细胞分别在缺氧条件下共培养,采用ELISA法检测培养基中HIF-1α和VEGF的表达情况,CCK8检测血管内皮细胞的增殖情况。【结果】miR-484 mimics处理后,SCG-7901细胞的增殖能力显著降低,而miR-484 inhibitor处理组的细胞增殖能力则显著提高。与miR-NC组比较,miR-484 mimics组SCG-7901细胞的HIF-1α、VEGF mRNA和蛋白表达下调(均P<0.01),miR-484 inhibitor组的结果与之相反。在缺氧条件下,与miR-NC组相比,miR-484 mimics显著降低了培养基中HIF-1α和VEGF水平(均P<0.05),血管内皮细胞增殖能力降低(P<0.05),而miR-484 inhibitor组的结果与之相反。【结论】miR-484可能通过下调HIF-1α/VEGF通路抑制胃癌细胞增殖和肿瘤血管形成。

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关键词 ：胃肿瘤, 新生血管化, 病理性, 微RNAs, 信号传导, 血管内皮生长因子类

Abstract：【Objective】To investigate whether miR-484 participates in gastric cancer angiogenesis by regulating the HIF-1α/VEGF signaling pathway.【Methods】SCG-7901 gastric cancer cells were transfected with miR-NC, miR-484 mimics, and miR-484 inhibitors separately. The expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) mRNA and protein, as well as the proliferation of gastric cancer cells, were measured in each group. The individual SCG-7901 cells with successfully transfected miR-NC,miR-484 mimics and miR-484 inhibitors were co-cultured with vascular endothelial cells under hypoxic conditions. Then, ELISA was used to detect the expression of HIF-1α and VEGF in the culture medium, and CCK8 was used to assess the proliferation of vascular endothelial cells.【Results】After transfection with miR-484 mimics, the proliferation capacity of SCG-7901 cells was significantly reduced, while the proliferation capacity of cells in the miR-484 inhibitor group was significantly increased. Compared to the miR-NC group, HIF-1α and VEGF mRNA and protein expression levels were downregulated in the miR-484 mimics group (all P<0.01), while the opposite results were observed in the miR-484 inhibitor group. Under hypoxic conditions, the levels of HIF-1α and VEGF in the culture medium of the miR-484 mimics group were much lower than those of the miR-NC group (both P<0.05), while the proliferation of vascular endothelial cells was higher (P<0.05). The results of the miR-484 inhibitor group were the opposite.【Conclusion】The miR-484 inhibits gastric cancer cell proliferation and tumor angiogenesis by downregulating the HIF-1α/VEGF pathway.

Key words： Stomach Neoplasms Neovascularization,Pathologic MicroRNAs Signal Transduction Vascular Endothelial Growth Factors

收稿日期: 2024-06-28

中图分类号:

R735.2

基金资助:* 江苏省卫生健康委科研项目(ZD2022052);宿迁市科学技术局,宿迁市科技计划资助(S202117)

通讯作者: ** E-mail:1026360563@qq.com

引用本文:

刘瑾, 李士猛, 曹主根, 舒坚, 刘云云. miR-484通过调控HIF-1α/VEGF信号通路对胃癌血管生成的影响^*[J]. 医学临床研究, 2024, 41(9): 1289-1292.
LIU Jin, LI Shimeng, CAO Zhugen, et al. The Effect of miR-484 on Gastric Cancer Angiogenesis by Regulating the HIF-1α/VEGF Signaling Pathway. JOURNAL OF CLINICAL RESEARCH, 2024, 41(9): 1289-1292.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2024.09.003 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2024/V41/I9/1289

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