Abstract：【Objective】To investigate the effect of targeted knockout of ZNF217 gene expression by shRNA vector on invasion and apoptosis of human neuroblastoma Kelly . 【Methods】The shRNA hairpin structure of ZNF217 gene was constructed by siRNA technology. The human neuroblastoma cell line Kelly cells were divided into blank control group (group A), blank vector virus group (group B) and ZNF217 gene silencing expression vector lentivirus group (Group C). The expression levels of ZNF217, caspase-3 and caspase-9 were detected by RT-PCR and Western blot. Transwell kit was used to detect cell invasion, AV-PI kit to detect apoptosis, CCK-8 kit to detect cell proliferation. 【Results】The transfection titer of lentivirus was 3×107 TU/mL, and the transfection efficiency was over 92%. RT-PCR and Western blot showed that the relative expression of ZNF217 mRNA and protein in the group C was lower than that in the group A and B (P<0.05). However, the relative expression of Caspase-3 and caspase-9 in the group C was higher than that in the group A and B (P<0.05). The cell proliferation rate of the group C was significantly lower than that of the group B and the group A (P<0.05), and the cell invasion ability of the group C was significantly lower than that of the group B and the group A (P<0.05). The apoptosis rate of the group C was significantly higher than that of the group B and the group A (P<0.05). 【Conclusion】Targeted knockout of ZNF217 gene expression by shRNA vector can inhibit the proliferation, invasion and apoptosis of human neuroblastoma cell line Kelly cells, so ZNF217 gene can be used as a potential therapeutic target for neuroblastoma.
唐湘莲, 文佳冰, 黎明, 肖雅玲, 李勇. 慢病毒介导shRNA载体定向敲除ZNF217基因表达对人神经母细胞瘤KELLY细胞侵袭和凋亡的影响[J]. 医学临床研究, 2019, 36(12): 2295-2298.
TANG Xiang-lian, WEN Jia-bing, LI Ming, et al. Lentivirus-mediated shRNA Vector Targeted Knockout of ZNF217 Gene Expression on Invasion and Apoptosis of Human Neuroblastoma KELLY Cells. JOURNAL OF CLINICAL RESEARCH, 2019, 36(12): 2295-2298.
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2019, Vol. 36 
