Abstract：【Objective】To explore the inhibition of roxithromycin on the proliferation of HaCaT keratinocyte cells induced by interleukin-22 (IL-22) .【Methods】Firstly, HaCaT cell viability was detected by MTT after cells were cultured with IL-22 at concentrations of 0, 12. 5, 25, 50, 100 and 200 μg/mL for 48h. Secondly, HaCaT cell viability was also detected by MTT after cells were cultured with roxithromycin at concentrations of 0, 10, 20, 40, 80, 160 and 320 μg/mL for 48h. Lastly, experiments were divided into the control group (vehicle treatment) , the model group (100 μg/mL IL-22) and roxithromycin groups at low, medium and high-dose groups (100, 300, 1000 μg/mL+100 μg/mL IL-22, respectively) . The cell viability was measured by MTT methodand expressions of cytokeratin16 (K16) , K17, Bax and Bcl-2 were detected by Western blot.【Results】Cell viability of HaCaT was increased by IL-22 at concentrations of 12. 5, 25, 50 and 100 μg/mL. However, the cell viability was decreased by IL-22 at a concentration of 200 μg/mL. Similarly, cell viability was increased by roxithromycin at concentrations of 10、20 μg/mL, but the cell viability was decreased by roxithromycin at concentrations of 40、80、160、320 μg/mL. Compared to the vehicle control group, while the cell viability of IL-22 treated model group was increased, the expression of K16, K17 and Bcl-2 was up-regulated (P＜0.01) , the expression of Bax was down-regulated. Compared to the model group, the cell viability in low, medium and high-dose of roxithromycin groups was decreased, and the expression of K17 was down-regulated. The expression of Bax was up-regulated (P＜0.01) . The expression of K16 and Bcl-2 was down-regulated in roxithromycin medium and high-dose groups.【Conclusion】Roxithromycin can inhibit IL-22-induced proliferation of HaCaT keratinocyte cells by down-regulating the expression of cytokeratin and regulating the expression of cell apoptotic related proteins.
杨今言,关晓燕,陈赵慧,卢娟,李丽华,宋珍珍. 罗红霉素对IL-22诱导的HaCaT角质形成细胞增殖的影响[J]. 医学临床研究, 2017, 34(11): 2098-2100,2103.
YANG Jin-yan, GUAN Xiao-yan, CHEN Zhao-hui, et al. Effect of Roxithromycin on the Proliferation of HaCaT Keratinocytes Induced by IL-22. JOURNAL OF CLINICAL RESEARCH, 2017, 34(11): 2098-2100,2103.
2017, Vol. 34 
