Abstract：【Objective】 To investigate the D gene polymorphism in RhD negative pregnant women in the Shaanxi Han nationality.【Methods】 From January 2018 to March 2020,54 Han women in Shaanxi who were RhD negative during the initial screening were selected for regular prenatal examination in our hospital. RhD blood group was detected by microcolumn gel card; Indirect antiglobulin test (IAT) was performed by anti human globulin gel card method; RhCE antigen typing was identified using Rh typing cards; Del blood group was detected by absorption radiation test; Sequence specific polymerase chain reaction (PCR-SSP) was used to identify the Del blood group genotype of the specimens; The reaction pattern table of D-Screen reagent was used to identify some types of D Ⅵ 3. PCR amplification and sequencing were performed on 10 specific primers for RHD gene exons. 【Results】 Of the 54 pregnant women who were RhD negative in the initial screening,3 were positive for monoclonal (IgG+IgM) anti D antibody IAT test and identified as D variant. Acid diffusion method detected 18 positive cases,except for the above 3 cases of D variant,the remaining 15 cases were all Del phenotypes,and 36 cases were D negative samples. Neither the D variant nor the Del phenotype produced anti D,and 2 of the 36 D negative specimens produced anti JKb combined with anti CE,while 7 produced anti D (1 case of them produced anti C combined with anti D). The RhCE typing results showed that the D negative blood group was mainly ccee,the Del phenotype was mainly Ccee,and there were no ccEe and ccee. Among the 3 cases of D variant,D-Screen detected 2 cases of partial D Ⅵ 3 type,and the remaining 1 case was identified as weak D15 type by RHD gene sequencing analysis. The RHD gene had a homozygous mutation of c.845G>(p.Gly282Asp). PCR-SSP detected 15 positive cases,consistent with the number of positive cases detected by acid diffusion method. The 15 Del phenotypes all carried RHD * 01EL.01. Among the 15 Del phenotypes,there were D+/D+1 cases with a zygotic genotype of RHD * 01EL.01/01EL.01; There were 14 cases of homozygous type D+/D-14,and their genotype was RHD * 01EL01/01N.01,accounting for 93.33%. 【Conclusion】 There are complex polymorphisms in the structure of D gene in 54 cases of RhD negative pregnant women in the Han nationality of Shaanxi province. The variant allele D negative blood group is mainly ccee,and the Del phenotype is mainly Ccee.
雍乐, 刘新, 王安, 王川. 陕西汉族初筛RhD阴性孕妇中D基因多态性研究[J]. 医学临床研究, 2023, 40(3): 411-413.
YONG Le, LIU Xin, WANG An, et al. Study on D Gene Polymorphism in RhD Negative Pregnant Women of the Han Nationality in Shaanxi Province. JOURNAL OF CLINICAL RESEARCH, 2023, 40(3): 411-413.
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2023, Vol. 40 
