丙酮酸脱氢酶激酶4和转录因子同源盒A1在胃癌组织及癌旁组织中的表达及其意义穆金姬<sup>a</sup> 樊雷<sup>b</sup>

doi:10.3969/j.issn.1671-7171.2022.05.012

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摘要【目的】探讨丙酮酸脱氢酶激酶4(PDK4)和转录因子同源盒A1(HOXA1)在胃癌组织及癌旁组织中的表达情况及其意义。【方法】选取2019年1月至2021年7月在本院确诊并接受手术治疗的123例胃癌患者,标本采集于胃部癌性病变部位组织(胃癌组)及毗邻癌性病变的癌旁组织(癌旁组)。采用实时荧光定量聚合酶链式反应(qRT-PCR)测定胃癌及其癌旁组织中PDK4 mRNA、HOXA1 mRNA的表达水平,采用免疫组织化学法检测PDK4和转录因子HOXA1的表达情况,分析胃癌组织PDK4、HOXA1表达水平与胃癌患者的相关病理参数的关系;分析胃癌组织PDK4与HOXA1的相关性。所有患者随访3年,采用Kaplan-Meier法分析PDK4、HOXA1表达与胃癌患者预后不良的关系,采用COX回归分析影响胃癌患者预后不良的危险因素。【结果】在胃癌组织中PDK4 mRNA、HOXA1 mRNA表达水平以及蛋白阳性率明显高于癌旁组织(P<0.05);PDK4表达与患者浸润深度、TNM分期、分化程度、淋巴结转移有关(P<0.05),HOXA1表达与患者浸润深度、肿瘤大小、TNM分期、分化程度、淋巴结转移有关(P<0.05);胃癌组织中PDK4表达、HOXA1表达呈正相关(P<0.05),Kaplan-Meier结果显示PDK4、HOXA1阳性表达患者的生存率(26.0%、23.6%)低于阴性表达的生存率(63.4%、65.9%)。多因素COX分析显示,PDK4、HOXA1阳性是影响胃癌患者预后不良的危险因素(P<0.05)。【结论】PDK4、HOXA1表达可能与胃癌的发生及预后有关,二者在胃癌中的表达密切相关,对评估胃癌的预后具有重要意义。

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	穆金姬
	樊雷
	杨剑

关键词 ：胃肿瘤/病理学, 基同源盒结构域蛋白质类, 基因表达调控,酶学, 免疫组织化学

Abstract：【Objective】 To investigate the expression of pyruvate dehydrogenase kinase 4 (PDK4) and transcription factor homeobox A1 (HOXA1) in gastric cancer and adjacent tissues. 【Methods】 A total of 123 patients with gastric cancer who were diagnosed and treated surgically in our hospital from January 2019 to July 2021 were selected. The specimens were collected from the tissues of cancerous lesions in the stomach (gastric cancer group) and adjacent cancerous tissues (adjacent cancer group). The expression levels of PDK4 mRNA and HOXA1 mRNA in gastric cancer and its adjacent tissues were measured by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The expressions of PDK4 and transcription factor Hoxa1 were detected by immunohistochemistry. The relationship between the expression levels of PDK4 and HOXA1 in gastric cancer and the related pathological parameters of gastric cancer patients was analyzed; The correlation between PDK4 and HOXA1 in gastric cancer was analyze. All patients were followed up for 3 years. Kaplan Meier method was used to analyze the relationship between the expression of PDK4 and HOXA1 and the poor prognosis of patients with gastric cancer. COX regression was used to analyze the risk factors affecting the poor prognosis of patients with gastric cancer. 【Results】 The expression levels of PDK4 mRNA and HOXA1 mRNA and the positive rate of protein in gastric cancer tissues were significantly higher than those in adjacent tissues (P<0.05); The expression of PDK4 was related to the depth of invasion, TNM stage, degree of differentiation and lymph node metastasis (P<0.05), and the expression of HOXA1 was related to the depth of invasion, tumor size, TNM stage, degree of differentiation and lymph node metastasis (P<0.05); There was a positive correlation between the expression of PDK4 and HOXA1 in gastric cancer (P<0.05). Kaplan Meier results showed that the survival rate of patients with positive expression of PDK4 and HOXA1 (26.0%, 23.6%) was lower than that of patients with negative expression (63.4%, 65.9%). Multivariate COX analysis showed that positive PDK4 and HOXA1 were risk factors for poor prognosis in patients with gastric cancer (P<0.05). 【Conclusion】 The expression of PDK4 and HOXA1 may be related to the occurrence and prognosis of gastric cancer. The expression of PDK4 and HOXA1 in gastric cancer is closely related, which is of great significance to evaluate the prognosis of gastric cancer.

Key words： Stomach Neoplasms/PA Homeodomain Proteins Gene Expression Regulation, Enzymologic Immunohistochemistry

收稿日期: 2021-12-14

中图分类号:

R735.2

通讯作者: *E-mail:270441397@qq.com

引用本文:

穆金姬, 樊雷, 杨剑. 丙酮酸脱氢酶激酶4和转录因子同源盒A1在胃癌组织及癌旁组织中的表达及其意义穆金姬^a 樊雷^b[J]. 医学临床研究, 2022, 39(5): 682-685.
MU Jin-ji, FAN Lei, YANG Jian. Expression and Significance of Pyruvate Dehydrogenase Kinase 4 and Transcription Factor Homeobox A1 in Gastric Cancer and Adjacent Tissues. JOURNAL OF CLINICAL RESEARCH, 2022, 39(5): 682-685.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2022.05.012 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2022/V39/I5/682

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