Abstract：【Objective】 To investigate the association between HLA-G allele polymorphism and the incidence of rheumatoid arthritis (RA) and the production of rheumatoid factor (RF). 【Methods】 through confirmed 95 cases of RA patients and 110 health examiner, to extract all the object of study after peripheral blood, with the method of polymerase chain reaction sequence specific primers (PCR-SSP method) on the research object of HLA-G allele specific amplification and sequencing of the HLA-G type don't statistical analysis, compare the HLA -G allele in the distribution of the frequency of the differences between different groups, and chi-square test for statistical analysis. 【Results】 There was no significant difference in the distribution frequency of four HLA-G alleles between RA group and control group (P>0.05).There was no significant difference in the distribution frequency of four HLA-G alleles between RF positive and RF negative patients in RA group (P>0.05). 【Conclusion】 No difference in the distribution frequency of HLA-G allele was found between RA patients and the normal population, and no protective or susceptible HLA-G allele associated with RA was found. HLA-G may not be directly related to the incidence of RA and the generation of RF.
吴菲, 刘婷, 严湘红. HLA-G基因多态性与类风湿关节炎的相关性研究[J]. 医学临床研究, 2021, 38(4): 556-558.
WU Fei, LIU Ting, YAN Xiang-hong. Study on the Association Between HLA-G Gene Polymorphism and Patients with Rheumatoid Arthritis. JOURNAL OF CLINICAL RESEARCH, 2021, 38(4): 556-558.
[1] 王东梅, 刘娜, 王丽君,等. 北方汉族MICA基因遗传多态性及与HLA-B连锁不平衡研究[J].中国输血杂志, 2019, 32(05):19-22.
[2] CHO K, KOOK H, KANG S, et al. Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy[J].J Extracell Vesicle,2020,9(1):1795364.
[3] VIATTE S, PLANT D, HAN B, et al. Association of HLA-DRB1 Haplotypes With Rheumatoid Arthritis Severity, Mortality, and Treatment Response[J].JAMA,2015, 313(16):1645-1656.
[4] TILBURGS T, CRESPO N C, ZWAN A V D, et al. Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes[J].Proc Natl Acad Sci USA,2015, 112(23):7219-7224.
[5] 施小明, 叶冬青, 陆伟,等. 安徽地区皖籍汉族人群HLA-G基因多态性[J].中国预防医学杂志, 2002, 3(2):92-94.
[6] RENAN G G,DE BRITO C A,VALÉRIA F M,et al. HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression[J].Hum Immunol,2018,79(6):477-484.
[7] 慕丽娟,袁敏,唐元家 等. HLA-G基因3′UTR区14bp的多态性与上海地区中国人群SLE的关联性[J].现代免疫学, 2009,29(4):270-273.
[8] 朱春燕, 聂咏梅, 周豪杰. PCR-SSP法检测HPA-15基因频率的可靠性分析[J].热带医学杂志, 2015,15(7):897-898.
[9] MAGALHÃES K C,SILVA K R,GOMES N A, et al. HLA-G 14 bp In/Del and +3142 C/G genotypes are differentially expressed between patients with grade Ⅳ gliomas and controls[J].Int J Neurosci,2020, 131(4):327-335.
[10] 刘洪福, 张蕾, 阳书华,等. 汉族人HLA-G基因多态性与胃癌相关性研究[J].实用临床医药杂志, 2017, 21(19): 70-72.
[11] VEIT T D, VIANNA P, ILÓITE S, et al. Association of the HLA-G 14-bp insertion/deletion polymorphism with juvenile idiopathic arthritis and rheumatoid arthritis[J].Tissue Antigens,2008, 71(5):440-446.
[12] KIM S K, CHUNG J H, KIM D H, et al. Lack of association between promoter polymorphisms of HLA-G gene and rheumatoid arthritis in Korean population[J].Rheumatol Int,2012,32(2):509-512.
[13] KRONER A.The genetic influence of the nonclassical MHC molecule HLA-G on multiple sclerosis[J].Human Immunol,2007, 68(5):422-425.
[14] 胡兰英. 抗环瓜氨酸肽抗体联合类风湿因子检测对类风湿关节炎的诊断价值[J].临床医学研究与实践, 2020,5(19):92-94.
2021, Vol. 38 
