Expression of PAX8, ASAH1 and Cullin7 in Tumor Tissues and Adjacent Tissues of Patients with Gliomatosis Cerebri and their Correlation with Survival Status
摘要【目的】探究配对盒基因8(PAX8)、酸性神经酰胺酶(ASAH1)、Cullin7在脑胶质瘤(GC)患者癌组织与癌旁组织中的蛋白表达情况及与生存状况的相关性。【方法】选取2014年1月至2017年7月本院收治的75例GC患者,均行手术切除,比较癌组织与癌旁组织中PAX8、ASAH1、Cullin7蛋白表达情况,分析三者表达之间相关性,并分析三者与临床病理特征的相关性。分析PAX8、ASAH1、Cullin7蛋白表达与患者3年生存率的相关性。【结果】癌组织中PAX8、ASAH1、Cullin7蛋白阳性表达率高于癌旁组织,差异有统计学意义( P <0.05);PAX8蛋白与ASAH1蛋白呈正相关,ASAH1蛋白与Cullin7蛋白呈正相关,Cullin7蛋白与PAX8蛋白呈正相关( P <0.05);PAX8、ASAH1、Cullin7蛋白与GC患者病理类型、病理分级、分化程度、浸润深度、瘤周水肿显著相关( P <0.05); PAX8、ASAH1、Cullin7蛋白阳性表达与3年生存率呈负相关( P <0.05)。【结论】GC患者癌组织PAX8蛋白、ASAH1蛋白、Cullin7蛋白均呈阳性表达,并与生存状况存在负相关性。
Abstract：【Objective】To investigate the protein expression of paired box gene 8 (PAX8), acid ceramidase (ASAH1) and Cullin7 in the cancerous tissues and adjacent normal tissues of patients with gliomatosis cerebri (GC) and their correlation with survival. 【Methods】A total of patients with GC in our hospital from January 2014 to July 2017 were selected, all of them underwent surgical resection. The protein expressions of PAX8, ASAH1 and Cullin7 in cancer tissues and adjacent tissues were compared, and the correlation between the expression of PAX8, ASAH1 and Cullin7 was analyzed, as well as the correlation between the expression of PAX8, ASAH1 and Cullin7 and the clinicopathological features. The correlation between PAX8, ASAH1, Cullin7 protein expression and 3-year survival rate was analyzed.【Results】 The positive expression rates of Pax8, ASAH1 and cullin7 in cancer tissues were higher than those in adjacent tissues ( P <0.05); PAX8 protein was positively correlated with ASAH1 protein, ASAH1 protein was positively correlated with cullin7 protein, Cullin7 protein was positively correlated with Pax8 protein ( P <0.05); the expression of PAX8, ASAH1 and Cullin7 protein was significantly correlated with pathological type, pathological grade, differentiation degree, depth of invasion and peritumoral edema ( P <0.05); the positive expression of PAX8, ASAH1 and Cullin7 protein was negatively correlated with 3-year survival rate ( P <0.05).【Conclusion】 PAX8 protein, ASAH1 protein and Cullin7 protein are all positive in GC patients, and there is a negative correlation with survival status.
张莞萍, 李长生. PAX8、ASAH1、Cullin7在脑胶质瘤患者癌组织与癌旁组织中的蛋白表达及与生存状况的相关性分析[J]. 医学临床研究, 2021, 38(3): 413-415.
ZHANG Guan-ping, LI Chang-sheng. Expression of PAX8, ASAH1 and Cullin7 in Tumor Tissues and Adjacent Tissues of Patients with Gliomatosis Cerebri and their Correlation with Survival Status. JOURNAL OF CLINICAL RESEARCH, 2021, 38(3): 413-415.
[1] Georgakis MK,Spinos D,Pourtsidis A, et al .Incidence and survival of gliomatosis cerebri: a population-based cancer registration study[J]. J Neurooncol, 2018,138(2):341-349. [2] 乔晓霞. ASAH1、TGFβ1、Caspase-3在人脑胶质瘤组织中表达及相关性研究[D].石家庄:河北大学,2018. [3] Zhang D,Yang G,Li X, et al .Inhibition of Liver Carcinoma Cell Invasion and Metastasis by Knockdown of Cullin7 In Vitro and In Vivo[J]. Oncol Res, 2016,23(4):171-181. [4] 唐海峰,李贤,刘蓓.敲降HE4和PAX8基因对TC方案治疗上皮性卵巢癌细胞恶性生物学行为的影响[J].中国肿瘤生物治疗杂志,2019,26(7):757-761. [5] 樊春波,王晶,蒋勇,等.ApoE和IL-18基因多态性与脑胶质瘤发生的临床关系[J].基因组学与应用生物学,2018,37(10):4574-4580. [6] Xu J,Zhang Z,Qian M, et al .Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation[J]. J Exp Clin Cancer Res, 2020,39(1):59. [7] 向旭,谢腾,牟宇,等.Cullin7蛋白在人脑胶质瘤中的表达及临床意义[J].中国临床神经外科杂志,2019,24(12):746-748. [8] 林兰,李乾.Cullin7蛋白在原发性肝癌癌组织中的表达及其与临床病理特征的关系[J].新疆医科大学学报,2019,42(2):221-225. [9] Bie LY,Li N,Deng WY, et al . Evaluation of PAX8 expression promotes the proliferation of stomach Cancer cells[J]. BMC Mol Cell Biol, 2019,20(1):61. [10] 宋艳,黄璇,沈贵华,等.配对盒基因8和配对盒基因2蛋白在上皮及肿瘤组织中的表达差异[J].中华肿瘤杂志,2017,39(6):424-428. [11] Leclerc J,Garandeau D,Pandiani C, et al .Lysosomal acid ceramidase ASAH1 controls the transition between invasive and proliferative phenotype in melanoma cells[J]. Oncogene, 2019,38(8):1282-1295. [12] Vethakanraj HS,Babu TA,Sudarsanan GB, et al .Targeting ceramide metabolic pathway induces apoptosis in human breast cancer cell lines[J]. Biochem Biophys Res Commun ,2015,464(3):833-839. [13] 乔晓霞. 脑胶质瘤组织中ASAH1,TGF-β1,Caspase-3蛋白表达变化及其意义[J].山东医药,2018,58(15):75-77.
2021, Vol. 38 
