Abstract：【Objective】To establish a mouse histiocytosarcoma model and screen a high invasive tumor strain. The expression of IGF-1 and MMP-10 protein in the tumor strain and clinical specimens were detected and the clinical significance was explored. 【Methods】 Primary L-II tumor cells were inoculated subcutaneously into the axilla of the mice, and the lung metastases were taken to make a suspension to inoculate another mouse, and then the lung metastases were taken for further passage. A mouse xenograft model was established using primary cells and ninth generation cells, respectively. The survival time and tumor size of primary tumor and metastatic tumor were compared. Immunohistochemistry and Western blot were used to detect the expression of IGF-1 and MMP-10. 【Results】 Mouse histiocytic sarcoma L-II to passage 9 were screened by serial transfer in vivo. The results showed that the selected tumor strains grew faster than the pre-screened tumor strains. The survival time of mice before and after screening was 29.23±2.90 days and 23.66±2.97 days, respectively. The difference was significant (P<0.05). The expression of IGF-1 and MMP-10 protein in the 9th generation tumor strain of mice was up-regulated compared with the expression of the tumor strain before screening. 【Conclusion】 The expressions of IGF-1 and MMP-10 were both enhanced in the mouse model of the 9th generation tumor cells, which suggests that IGF-1 and MMP-10 may play a role in the process of histiocytosarcoma metastasis.
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2019, Vol. 36 
