SIK1、SIK2在甲状腺微小癌及癌旁组织中的表达及其意义

doi:10.3969/j.issn.1671-7171.2019.05.010

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摘要 【目的】探讨盐诱导蛋白激酶1(SIK1)、盐诱导蛋白激酶2(SIK2)在甲状腺微小癌(PTMC)及癌旁组织中的表达及其临床意义。【方法】收集2014年1月至2016年12月本院术后病理确诊的PTMC及配对癌旁组织标本80例,检测SIK1、SIK2在PTMC及癌旁组织中的表达水平,分析其与PTMC患者年龄、性别、肿瘤TNM分期、癌灶直径大小、脉管浸润、神经周围浸润等临床资料的相关性。【结果】免疫组化结果显示:与癌旁组织比较,癌组织SIK1、SIK2蛋白表达水平较高(P<0.05);PCR结果显示:SIK1 mRNA在癌组织的表达水平显著高于癌旁组织(P<0.05),SIK2 mRNA在癌组织及癌旁组织中的表达水平比较差异无统计学意义(P>0.05)。logistic回归分析显示:PTMC组织中SIK1表达与癌灶直径大小、是否发生淋巴转移、是否发生远处转移、是否发生脉管浸润呈正相关(P<0.05);PTMC组织中SIK2的表达水平与癌灶直径大小、是否发生淋巴转移、是否发生远处转移呈正相关(P<0.05)。【结论】癌组织中的SIK1 、SIK1蛋白表达水平显著高于癌旁组织,且与癌灶直径大小、淋巴转移、远处转移灶呈正相关,提示SIK1 、SIK1与PTMC的进展和预后有关,可作为PTMC诊疗的新指标。

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	黄波
	郑剑忠
	刘金煌
	许毓敏

关键词 ：甲状腺肿瘤/病理生理学, 蛋白质类

Abstract：【Objective】To investigate the expression and clinical significance of SIK1 (salt-induced protein kinase 1) and SIK2 (salt-induced protein kinase 2) in thyroid microcarcinoma and adjacent tissues. 【Methods】 A total of 80 cases of pathology-confirmed thyroid microcarcinoma and matched paracancerous tissues from January 2014 to December 2016 were collected from the specimens bank of thyroid cancer in Shenzhen Baoan District People's Hospital. The expression levels of SIK1 and SIK2 in thyroid microcarcinoma and adjacent tissues were detected by immunohistochemistry and Q-PCR. The correlations of gene expression with patients' age, gender, tumor diameter, TNM stage and vascular invasion were analyzed.【Results】The results of immunohistochemistry showed that the expression levels of SIK1 and SIK2 in cancer tissues were higher than those in adjacent tissues (P<0.05). The results of PCR showed that the expression level of SIK1 in cancer tissue was significantly higher than that in adjacent tissues (P<0.05). However, there was no significant difference in the expression level of SIK2 between cancer tissues and adjacent tissues (P>0.05). Logistic regression analysis showed that the expression level of SIK1 in thyroid microcarcinoma tissue was positively correlated with tumor diameter, vascular invasion, lymphatic metastasis and distant metastasis (P<0.05). Logistic regression analysis also showed that the expression level of SIK2 was positively correlated with tumor size, lymphatic metastasis and whether distant metastasis (P<0.05). 【Conclusion】The expression levels of SIK1 and SIK2 in thyroid microcarcinoma tissues are significantly higher than those in tumor adjacent tissues. The levels of SIK1 and SIK2 expression are positively correlated with tumor diameter, lymphatic metastasis and distant metastasis, suggesting that SIK1 and SIK2 are related to the progression and prognosis. SIK1 and SIK2 can be used as new indicators of thyroid microcarcinoma diagnosis and treatment.

Key words： Thyroid Neoplasms/PP Proteins

收稿日期: 2019-03-04

PACS:

R736.1

基金资助:深圳市宝安区科技创新局项目(项目编号:2018JD185)

引用本文:

黄波, 郑剑忠, 刘金煌, 许毓敏. SIK1、SIK2在甲状腺微小癌及癌旁组织中的表达及其意义[J]. 医学临床研究, 2019, 36(5): 863-865.
HUANG Bo, ZHENG Jian-zhong, LIU Jin-huang, et al. Expression and Clinical Significance of SIK1 and SIK2 in Thyroid Microcarcinoma and Adjacent Tissues. JOURNAL OF CLINICAL RESEARCH, 2019, 36(5): 863-865.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2019.05.010 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2019/V36/I5/863

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