医学临床研究
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医学临床研究  2019, Vol. 36 Issue (4): 689-692    DOI: 10.3969/j.issn.1671-7171.2019.04.023
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整合素α5和caspase 3在肝细胞癌中的表达情况及意义
刘文平
绵阳市第三人民医院肝胆胰外科,四川 绵阳 621000
Expression and Significance of Integrin α 5 and caspase 3 in Hepatocellular Carcinoma
LIU Wen-ping
Mianyang Third People's Hospital,Sichuan Province 621000
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摘要 【目的】探讨整合素α5和含半胱氨酸的天冬氨酸蛋白水解酶3(caspase 3)在肝细胞癌(HCC)中的表达及其临床意义。【方法】收集2015年1月至12月40例在本院实施手术切除并经病理确诊为HCC病例的癌组织石蜡标本以及癌旁组织标本,备行免疫组织化学检查整合素α5、caspase 3于HCC中的表达情况;收集2016年1月至12月期间选择实施手术切除的HCC患者30例的新鲜HCC组织和对应的癌旁组织,备行荧光定量RT-PCR和蛋白质印迹法检测整合素α5、easpase3的mRNA表达。【结果】肝癌组织中整合素α5表达率显著高于,caspase 3的阳性表达率低于癌旁组织(P<0.05);40例HCC患者的整合素α5蛋白质表达与TNM分期和肿瘤转移密切相关(P<0.05),caspase 3的蛋白质表达与HBsAg、TNM分期、肿瘤转移密切相关(P<0.05);.肝癌组织中整合素α5和caspase 3的表达具有负相关性(P<0.05);肝癌组织中的整合素α5 mRNA相对表达量明显高于癌旁组织,caspase 3 mRNA低于癌旁组织(P<0.05)。【结论】整合素α5与caspase 3呈负相关表达,其可能在肝癌细胞的凋亡期间进行参与,但它们对于肝癌细胞失巢凋亡的作用机制需要进一步的深入研究。
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刘文平
关键词 肝细胞/诊断整合素α5/诊断应用肿瘤蛋白质类/诊断应用    
Abstract【Objective】 To observe and analyze the expression and clinical significance of integrin α 5 and aspartate protease 3 containing cysteine (caspase 3) in patients with hepatocellular carcinoma(HCC). 【Methods】 Paraffin-embedded specimens of 40 HCC cancer and paracancerous tissues from January to December 2015 underwent surgical resection in our hospital HCC were collected. Immunohistochemical examination of integrin α 5 and caspase 3 expression was performed. Also, fresh HCC tissues and corresponding paracancerous tissues were collected from 30 HCC patients who underwent surgical resection between January to December, 2016. Fluorescence quantitative RT-PCR and Western blotting were used to detect integrin α 5 and caspase 3 expression.【Results】The expression rate of integrin α 5 in HCC was significantly higher than that in adjacent tissues, while the expression of caspase 3 in HCC was lower than that in paracancerous tissue (P<0.05). The expression of integrin α 5 protein in 40 patients with HCC was closely related to TNM stage and tumor metastasis (P<0.05), while caspase 3 protein expression was related to HBsAg,TNM stage and tumor metastasis (P<0.05). There was a negative correlation between integrin α 5 and caspase 3 expression in HCC (P<0.05). The relative expression of integrin α 5 mRNA in HCC was significantly higher than that in adjacent tissues.The caspase 3 mRNA in the liver cancerous tissue was significantly lower than that in the paracancerous tissue (P<0.05). 【Conclusion】The negative correlation between integrin α 5 and caspase 3 may be involved in the apoptosis of hepatoma cells. However, the mechanism of integrin α 5 on apoptosis of hepatoma cells requires further study.
Key wordsCarcinoma    Hepatocellular/DI    Integrin alpha5/TH    Neoplasm Proteins/TH
收稿日期: 2018-11-04     
PACS:  R735.7  
引用本文:   
刘文平. 整合素α5和caspase 3在肝细胞癌中的表达情况及意义[J]. 医学临床研究, 2019, 36(4): 689-692.
LIU Wen-ping. Expression and Significance of Integrin α 5 and caspase 3 in Hepatocellular Carcinoma. JOURNAL OF CLINICAL RESEARCH, 2019, 36(4): 689-692.
链接本文:  
http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2019.04.023     或     http://journal07.magtech.org.cn/yxlcyj/CN/Y2019/V36/I4/689
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