黏蛋白3A对肝外胆管癌细胞增殖的影响<sup>*</sup>

doi:10.3969/j.issn.1671-7171.2025.10.005

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摘要【目的】探讨黏蛋白3A(MUC3A)对肝外胆管癌(EHCC)细胞增殖的影响。【方法】选取表达水平较高的QBC939细胞进行细胞转染实验,采用瞬时转染,设计并合成针对MUC3A的shRNA干扰序列(#1和#2),重组慢病毒质粒进行包装,感染人肝外胆管癌QBC939细胞,将其分为四组并进行培养:未转染细胞(control)、空病毒转染细胞(shRNA-Ctrl) 及慢病毒介导shRNA转染细胞(shRNA-MUC3A#1、shRNA-MUC3A#2)。WB法检测shRNA对MUC3A的沉默效率。平板克隆形成实验检测敲低MUC3A对QBC939细胞增殖能力的影响。【结果】WB法检测结果显示,与control比较,shRNA-MUC3A#2、shRNA-MUC3A#1均呈下降趋势,shRNA-MUC3A#2具有较好的沉默效果,差异均有统计学意义(均P<0.05)。Transwell实验结果显示,转染24 h后,沉默MUC3A可显著降低QBC939 细胞的平板克隆形成,shRNA-MUC3A#2、shRNA-MUC3A#1均可抑制QBC939细胞增殖,其中shRNA-MUC3A#2抑制QBC939细胞增殖的能力更强,差异均有统计学意义(均P<0.05),说明敲低MUC3A可影响QBC939细胞增殖能力。【结论】MUC3A对EHCC细胞增殖具有重要的影响作用,敲低EHCC细胞内MUC3A可抑制EHCC细胞增殖。

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	刘鹏

关键词 ：胆管肿瘤, 胆管,肝外, 黏蛋白3, 细胞增殖

Abstract：【Objective】To explore the effect of MUC3A on the proliferation of extrahepatic cholangiocarcinoma (EHCC) cells. 【Methods】QBC939 cells with high MUC3A expression were selected for cell transfection. Transient transfection was used, and shRNA interference sequences (#1 and #2) targeting the MUC3A gene were designed and synthesized. Recombinant lentiviral plasmids were packaged to produce viral solution, which was then used to infect EHCC QBC939 cells. The cells were divided into four groups for culture: untransfected cells (controll), empty virus-transfected cells (shRNA-Ctrl), and lentivirus-mediated shRNA-transfected cells (shRNA-MUC3A#1, shRNA-MUC3A#2). Western Blot (WB) was used to detect the silencing efficiency of shRNA on the MUC3A gene. Colony formation assay was used to detect the effect of MUC3A knockdown on the proliferation ability of QBC939 cells. 【Results】WB results showed that compared with the control group, the MUC3A expression in the shRNA-MUC3A#1 and shRNA-MUC3A#2 groups was decreased, and shRNA-MUC3A#2 had a better silencing effect, with statistically significant differences (all P<0.05). Colony formation assay results showed that 24 hours after transfection, silencing MUC3A significantly reduced the colony formation of QBC939 cells; both shRNA-MUC3A#1 and shRNA-MUC3A#2 could inhibit the proliferation of QBC939 cells, and shRNA-MUC3A#2 had a stronger inhibitory effect, with statistically significant differences (all P<0.05), indicating that MUC3A knockdown affects the proliferation ability of QBC939 cells. 【Conclusion】 MUC3A plays an important role in the proliferation of EHCC cells; knocking down the expression of MUC3A protein in EHCC cells can inhibit the proliferation of extrahepatic cholangiocarcinoma cells.

Key words： Bile Duct Neoplasms Bile Ducts, Extrahepatic Mucin-3 Cell Proliferation

收稿日期: 2025-08-26

中图分类号:

R735.8

基金资助:* 湖南省卫生健康委员会科研课题(D202304019027)

引用本文:

刘鹏. 黏蛋白3A对肝外胆管癌细胞增殖的影响^*[J]. 医学临床研究, 2025, 42(10): 1696-1699.
LIU Peng. The Effect of MUC3A on the Proliferation of Extrahepatic Cholangiocarcinoma Cells. JOURNAL OF CLINICAL RESEARCH, 2025, 42(10): 1696-1699.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2025.10.005 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2025/V42/I10/1696

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