Abstract：【Objective】 To investigate the effects of miR-181a and miR-138 on the proliferation of chondrosarcoma cells. 【Methods】A total of 20 samples of osteosarcoma and 20 samples of chondrosarcoma tissues were collected. qRT PCR was used to detect the expression levels of miR-181a and miR-138. miRNA mimetics were used to promote the expression of miR-181a and miR-138 in chondrosarcoma cells SW1353. Cell counting was used to detect the effects of miR-181a or miR-138 overexpression alone or in combination on the growth ability of SW1353 cells. The binding sites of miR-181a, miR-138 and RAS family associated domain protein 8 (RASSF8) were predicted using the TargetScan database, and gene experiments were validated using dual luciferase reporter technology.【Results】Compared with chondrosarcoma tissue, the expression of miR-181a and miR-138 was significantly upregulated in chondrosarcoma tissue (P<0.05); Compared with the negative control group, the cell growth ability of the miR-181a and miR-138 overexpression groups was significantly increased (P<0.05), and the combined overexpression group of miR-181a and miR-138 showed the most significant upregulation of cell growth ability; According to predictions, both miR-181a and miR-138 have binding sites with RASSF8; Compared with the cotransfected wild-type RASSF8+scaffold group, the luciferase activity in the cotransfected wild-type RASSF8+miR-181a group and wild-type RASSF8+miR-138 group was significantly reduced (P<0.05); Compared with the cotransfected mutant RASSF8+scaffold group, there was no significant change in luciferase activity in the cotransfected mutant RASSF8+miR-181a group and the mutated RASSF8+miR-138 group (P>0.05). 【Conclusions】miR-181a and miR-138 may promote the growth of chondrosarcoma cells by inhibiting RASSF8.
曾旭凯, 何畔, 汪志军. miR-181a及miR-138对软骨肉瘤细胞增殖的影响*[J]. 医学临床研究, 2023, 40(12): 1825-1828.
ZENG Xukai,HE Pan,WANG Zhijun. The Effects of miR-181a and miR-138 on the Proliferation of Chondrosarcoma Cells. JOURNAL OF CLINICAL RESEARCH, 2023, 40(12): 1825-1828.
[1] WEINSCHENK R C, WANG W L, LEWIS V O. Chondrosarcoma[J]. J Am Acad Orthop Surg,2021, 29(13):553-562. [2] AMICHETTI M, AMELIO D, CIANCHETTI M, et al. A systematic review of proton therapy in the treatment of chondrosarcoma of the skull base[J].Neurosurg Rev,2010, 33(2):155-165. [3] SMOLARZ B, DURCZYNSKI A, ROMANOWICZ H, et al. miRNAs in cancer (Review of Literature)[J].Int J Mol Sci,2022, 23(5):2805. [4] SALIMINEJAD K, KHORRAM K H, SOLEYMANI F S, et al. An overview of microRNAs: Biology, functions, therapeutics, and analysis methods[J].J Cell Physiol,2019, 234(5):5451-5465. [5] 杜凌云,王善龙,王晓.前列腺癌组织中miRNA-17-3P表达及其临床价值[J].医学临床研究,2023,40(9):1296-1298. [6] NAZERI E, SAVADKOOHI M, MAJIDZADEH A K, et al. Chondrosarcoma: An overview of clinical behavior, molecular mechanisms mediated drug resistance and potential therapeutic targets[J].Crit Rev Oncol Hematol,2018, 131:102-109. [7] WHELAN J S, DAVIS L E. Osteosarcoma, Chondrosarcoma, and Chordoma[J].J Clin Oncol, 2018, 36(2):188-193. [8] WANG X, KACZOR K E, WONG D T. Salivary biomarkers in cancer detection[J].Med Oncol,2017, 34(1):7. [9] PANOUTSOPOULOU K, AVGERIS M, MAGKOU P, et al. miR-181a overexpression predicts the poor treatment response and early-progression of serous ovarian cancer patients[J].Int J Cancer,2020, 147(12):3560-3573. [10] YAN X, GAO M J, ZHANG P, et al. MiR-181a functions as an oncogene by regulating CCND1 in multiple myeloma[J].Oncol Lett,2020, 20(1):758-764. [11] LE F, LUO P, YANG Q O, et al. MiR-181a promotes growth of thyroid cancer cells by targeting tumor suppressor RB1[J].Eur Rev Med Pharmacol Sci,2017, 21(24):5638-5647. [12] WANG T, ZHANG H Y, WANG H W, et al. MiR-505-5p inhibits proliferation and promotes apoptosis of osteosarcoma cells via regulating RASSF8 expression[J].J BUON,2021, 26(2):599-605. [13] ZHANG L L, CHEN H X, HE F X, et al. MicroRNA-320a promotes epithelial ovarian cancer cell proliferation and invasion by targeting RASSF8[J].Front Oncol,2021, 11:581932.
2023, Vol. 40 
