Abstract：【Objective】To investigate the effect of andrographolide (AG) combined with pirfenidone (PFD) on hepatic stellate cell activation induced by transforming growth factor-β1(TGF-β1) and its mechanism . 【Methods】 Hepatic stellate cells (LX-2) were used as the research object. After routine in vitro culture, they were divided into control group, TGF-β1 Group , AG group, PFD group and AG + PFD group. MTT assay was used to determine the cell viability of five groups, RT-PCR was used to detect the mRNA expression levels of fiber markers and inflammatory markers, and Western blot was used to detect the related protein expression level of TGF -β/ Smad signaling pathway cells in each group.【Results】 Compared with TGF- β1 group, fiber markers(α- SMA mRNA, Collagen-ⅠmRNA and CTGF mRNA) inflammatory marker (IL-1βmRNA, IL-6 mRNA and TNF- αmRNA) and TGF-β/ Smad signaling pathway related proteins (p-Smad2, p-smad3) of the AG group, the PFD group and the AG + PFD group were significantly down regulated (mean P<0.05); Compared with the PFD group, the expression levels of α- SMA mRNA and IL-6 mRNA in the AG + PFD group were significantly down regulated (mean P<0.05).【Conclusion】 Compared with monotherapy, AG combined with PFD therapy has significant effect on the activation of hepatic stellate cells induced by TGF-β1, and plays a better role in anti hepatic fibrosis, and its mechanism may be related to the regulation of TGF-β/ Smad signaling pathway.
许光, 马体栋, 赵凡, 李碧香, 周崇高. 穿心莲内酯和吡非尼酮联合给药对TGF-β1诱导的肝星状细胞活化的影响及作用机制[J]. 医学临床研究, 2022, 39(2): 168-172.
XU Guang, MA Ti-dong, ZHAO Fan, et al. Study on the Effect and Mechanism of Andrographolide Combined with Pirfenidone on the Activation of Hepatic Stellate Cells Induced by TGF-β1. JOURNAL OF CLINICAL RESEARCH, 2022, 39(2): 168-172.
2022, Vol. 39 
