Abstract：【Objective】 Based on the high-throughput tumor data in TCGA and GEO, the expression and clinical value of CALHMs family genes such as CALHM2 in non-small cell lung cancer were analyzed. 【Methods】 Using online tools like Oncomine, GEPIA and UALCAN to analyze and verify the expression level of CALHMs (CALHM1-6) family genes in non-small cell lung cancer(NSCLC); Using Human protein Atlas (HPA) to analyze the protein level and location of CALHM2 in NSCLC tissues ; Using GEPIA and UALCAN to analyze the correlations between CALHM2 expression level and pathological stage, lymph node metastasis, p53 mutation and smoking history in patients with NSCLC; Using UALCAN to investigate promoter methylation of CALHM2 in NSCLC; Using Kaplan-Meier Plotter to analyze the relationship between CALHM2 expression level and prognosis of NSCLC including overall survival (OS), first-time progression survival (FP) and post-progression survival (PPS); Using LinkedOmics to analyze the genes that were significantly related to CALHM2 expression in NSCLC; Using Metascape to perform GO function enrichment with CALHM2 correlated genes. 【Results】 The expression of CALHM2 was significantly down-regulated in NSCLC tissue; CALHM2 protein was also significantly reduced in NSCLC tissue. The expression of CALHM2 showed no significant correlation with pathological stage, lymph node metastasis, p53 mutation and smoking history in NSCLC patients. Kaplan-Meier Plotter analysis showed that NSCLC patients with high CALHM2 expression had significantly better OS and FP than those with low CALHM2. LinckedOmics screened a total of 265 genes significantly related to CALHM2, and Metascape enrichment analysis showed that CALHM2 related genes were significantly enriched in lymphocyte activation and migration, immune activation-related processes, and cytokine release regulation. 【Conclusion】 CALHM2 is significantly downregulated in NSCLC and can be used as a prognostic marker for patients with NSCLC.
刘洁, 黄伟, 王磊. 利用生物信息学分析CALHM2在非小细胞肺癌中的表达及临床意义[J]. 医学临床研究, 2021, 38(2): 179-184.
LIU Jie, HUANG Wei, WANG Lei. Evaluating Expression and Clinical Value of CALHM2 in Non-small Cell Lung Cancer Through Bioinformatics Data Analysis. JOURNAL OF CLINICAL RESEARCH, 2021, 38(2): 179-184.
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2021, Vol. 38 
