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Quick Search     Adv Search 2022 Vol. 39, No. 1 Published: 2022-01-26   1 Effects of Leptin on Autophagy, VEGF Expression, Cell Proliferation, Migration and Lumen Formation of RF/6A Cells in Vitro LIU Zhe, WANG Yi, LI Rong, et al DOI: 10.3969/j.issn.1671-7171.2022.01.001 【Objective】To investigate the effects of Leptin on autophagy, vascular endothelial growth factor (VEGF) expression, cell proliferation, migration and lumen formation of RF/6A cells in vitro. 【Methods】RF/6A cells were cultured with different concentrations of Leptin(50 ng/mL Leptin, 100 ng/mL Leptin, 200 ng/mL Leptin) for 24 hours. The expression levels of microtubule associated protein 1 light chain 3(LC3),Beclin-1, p62 and VEGF were detected by Western blotting. After the RF/6A cells were treated with Leptin combined with autophagy inhibitor 3-methyladenine (3-MA) and chloroquine (CQ), cell proliferation was detected by CCK8 method, cell migration was detected by Transwell method, and lumen formation was detected by Matrigel method. 【Results】Compared with the control group, different concentrations of Leptin could up regulate the expression of LC3, Beclin-1 and VEGF, and inhibit the expression of p62 (P<0.05); Compared with Leptin group, 3-MA and CQ could inhibit cell proliferation, reduce the number of migrating cells and the number of lumen formation (P<0.05). 【Conclusion】Leptin can activate autophagy in RF/6A cells, promote VEGF expression, promote cell proliferation, migration and lumen formation. 2022 Vol. 39 (1): 1-4 [Abstract] ( 105 ) HTML (1 KB)  PDF (1689 KB)  ( 148 ) 5 Value of KL-6, SP-A and SP-D in Early Diagnosis and Prognosis Assessment of Interstitial Lung Disease in Children DUAN Xiao-jun, ZHANG Xi, AN Zhao-hui, et al DOI: 10.3969/j.issn.1671-7171.2022.01.002 【Objective】To analysis the value of Krebs von den Lungen-6 (KL-6), lung Surfactant Protein A (SP-A) and lung Surfactant Protein D (SP-D) for the early diagnosis and prognosis assessment of interstitial lung disease (ILD)in children. 【Methods】A total of 35 children with interstitial lung disease (ILD) hospitalized in Department of Respiratory Medicine of Hunan Children's Hospital from March 2016 to July 2020 were selected as the ILD group, while 40 children hospitalized with infectious pneumonia during the same period were selected as the pneumonia group and 40 healthy children volunteers were selected as the control group. Clinical data of children in the three groups were collected to detect pulmonary function indicators of children in the three groups. Kl-6 was detected by chemiluminescent enzyme immunoassay, and SP-A and SP-D were detected by enzyme-linked immunoassay. The value of all three factors in early diagnosis and prognosis evaluation of interstitial pulmonary disease was analyzed. 【Results】The levels of KL-6, SP-A and SP-D in ILD group were higher than those in pneumonia group and control group (P<0.01). The level of serum KL-6 was positively correlated with maximum vital capacity (VC max) and one second rate (FEV1/FVC) (rs=0.339, P<0.05), while the level of SP-A was negatively correlated with 25% maximum expiratory flow (MEF25) (rs=-0.252, P<0.05) and the level of SP-D was negatively correlated with VC max (rs=-0.214, P<0.05). Pulmonary interstitial lesion score was positively correlated with KL-6, SP-A and SP-D (rs=0.542、0.618、0.515,P<0.01). The AUC,sensitivity, specificity and Youden index of KL-6, SP-A and SP-D combined detection were 0.89, 83.6%, 97.2% and 0.81, respectively, indicating that the combined detection of three parameteers was better than the single detection in the diagnosis of ILD. KL-6, SP-A and SP-D were the influencing factors for the prognosis of ILD children(P<0.05). 【Conclusion】KL-6, SP-A and SP-D can be used as indicators of early diagnosis, disease severity and prognosis of interstitial lung disease in children, and their combined detection application can improve the diagnostic accuracy. 2022 Vol. 39 (1): 5-8 [Abstract] ( 99 ) HTML (1 KB)  PDF (1172 KB)  ( 83 ) 9 Short-term Efficacy Observation of Intravenous Administration of Cinobufalin Combined with Lenvastinib in the Treatment of Primary Liver Cancer Patients After TACE WANG Ying-dong, DING Hai-bin, HUANG Zi-chao, et al DOI: 10.3969/j.issn.1671-7171.2022.01.003 【Objective】To investigate the short-term efficacy of intravenous administration of bufalin combined with lenvastinib in the treatment of primary liver cancer (PLC) after transarterial chemoembolization (TACE). 【Methods】A total of 104 patients with PLC after TACE were randomly divided into the observation group and the control group, with 52 cases in each group. The control group was treated with lumphatinib, while the observation group was given intravenous administration of bufalin in addition to the control group. The therapeutic effect was evaluated after 4 months of continuous treatment and followed up for 12 months. The short-term anti-tumor efficacy, the changes of immune function and tumor markers before and after treatment, the safety and survival status of the two groups at 12 months were compared between the two groups. 【Results】The clinical control rate of the observation group was higher than that of the control group (P<0.05). After treatment, the levels of Th1/Th2 in the observation group were higher than those before treatment(P<0.05), while the levels of Th17/Treg in the observation group were lower than those before treatment(P<0.05). However, the levels of Th1/Th2 in the control group were lower than those before treatment (P<0.05) and levels of Th17/Treg in the control group were higher than those before treatment(P<0.05). The Th1/Th2 level of the observation group was higher than that in the control group (P<0.05), and Th17/Treg level in the observation group was lower than that in the control group (P<0.05). After treatment, levels of CEA and AFP in both groups were lower than those before treatment (P<0.05), and CEA and AFP levels in the observation group were lower than those in the control group (P<0.05). There was no significant difference in CEA and AFP levels between the two groups before treatment (P>0.05). The safety in the two groups were not significantly different (P>0.05). Also, there was no significant difference in the incidence of various adverse reactions between the two groups (P>0.05). The survival rate of the control group at 12 months was 70.59%, and survival rate of the observation group was 80.77%. There was no statistically significant difference in the survival curve between the two groups (P>0.05). 【Conclusion】The intravenous injection of cinnobufacin combined with lenvatinib in the treatment of PLC after TACE can effectively improve the short-term treatment efficacy, improve immune function, reduce the level of tumor markers and is well tolerated. 2022 Vol. 39 (1): 9-12 [Abstract] ( 98 ) HTML (1 KB)  PDF (1250 KB)  ( 72 ) 13 Correlation between Total Burden of Cerebrovascular Disease and Extracranial and Extracranial Arteriosclerosis in Stroke-free Patients WANG Hui, LI Yun-xia, LIU Meng, et al DOI: 10.3969/j.issn.1671-7171.2022.01.004 【Objective】To investigate the correlation and clinical characteristics between imaging markers and total load of cSVD and the degree of extracranial and extracranial arteriosclerosis in patients without stroke. 【Methods】The clinical features, carotid ultrasound, magnetic resonance imaging (MRI) and computed tomography angiography (CTA) of 200 stroke-free patients were collected. The total load score of cSVD imaging markers was performed. The degree of extracranial and intracranial arteriosclerosis was evaluated according to the scores of extracranial and intracranial arteriosclerosis; The baseline data and extracranial and extracranial arteriosclerosis scores of patients with different global cSVD total load scores were compared; The correlation between various cSVD imaging marker scores, cSVD total load score and extracranial and extracranial arteriosclerosis score was analyzed. 【Results】There were significant differences in age, high homocysteine, intracranial artery stenosis, carotid plaque, intracranial arteriosclerosis score, extracranial arteriosclerosis score and total extracranial and extracranial arteriosclerosis score among patients with different total cerebral cSVD load score (P<0.01). The whole brain cSVD total load score was positively correlated with the total scores of extracranial, intracranial and extracranial arteriosclerosis (rs=0.594, 0.401 and 0.535, respectively, P<0.01); The correlation between cSVD total load score in one hemisphere and ipsilateral extracranial, intracranial and extracranial arteriosclerosis scores was significantly greater than that with contralateral extracranial, intracranial and extracranial arteriosclerosis (ipsilateral rs=0.616, 0.465 and 0.612, and contralateral rs=0.495, 0.355 and 0.478, respectively, P<0.01). Except for the PVSs score in the semioval center, the scores of other cSVD imaging markers were positively correlated with the total scores of extracranial, intracranial and extracranial arteriosclerosis.【Conclusion】The more severe the intracranial and extracranial arteriosclerosis, the heavier the ipsilateral and whole brain cSVD load; Extracranial and extracranial arteriosclerosis was positively correlated with the degree of lesions of various cSVD except PVSs in the center of semioval. 2022 Vol. 39 (1): 13-17 [Abstract] ( 70 ) HTML (1 KB)  PDF (1192 KB)  ( 77 ) 18 Effect of Fasudil on Autophagy in Hippocampal CA1 Region and mTOR Pathway in Rats with Subarachnoid Hemorrhage CAO Hong-bin, Feng Le-xiao DOI: 10.3969/j.issn.1671-7171.2022.01.005 【Objective】To investigate the effect of fasudil on autophagy in hippocampal CA1 region and mTOR pathway in rats with subarachnoid hemorrhage (SAH). 【Methods】Eighteen SPF male SD rats were randomly divided into sham operation group (SHAM group,n=6), SAH group (n=6) and fasudil group (FAS group,n=6). In FAS group, fasudil hydrochloride 10 mg/(kg?d) was injected intraperitoneally for 3 days; the SHAM group and the SAH group were intraperitoneally injected with the same amount of normal saline at the same time, and the rats in the sham group did not puncture the blood vessels. Shuttle box test was used to detect the learning and memory ability of rats in each group; Hematoxylin eosin (HE) staining was used to observe the pathological changes of neurons in hippocampal CA1 area of rats in each group, and the number of normal neurons was calculated; The changes of autophagy protein [tube associated protein light chain 3 (LC3), silkworm isolate protein 1 (p62)] and phosphoinositide 3-kinase/mammalian rapamycin target protein (PI3K/mTOR) pathway proteins [PI3K, p-PI3K, mTOR, p-mTOR] in hippocampal CA1 region of rats in each group were detected by Western blotting. 【Results】The escape times of the sham group, the SAH group and the FAS group were (26.33±3.05), (18.52±2.03), (22.06±2.58) times, the escape time was (3.69±1.01) s, (8.77± 3.96) s and (6.05±2.51) s, and the number of normal nerve cells was (125.69±18.85), (63.03± 9.46) and (95.77±14.33) respectively, The ratio of LC3-Ⅱ/LC3-Ⅰ was (0.97±0.15)%, (0.38±0.06)%, (0.56±0.09)%, the expression of p62 protein was (0.45±0.07), (1.13±0.17), (0.81±0.13), p-PI3K/PI3K protein was 0.65±0.09,1.58±0.24, 1.03±0.15, p-mTOR/mTOR was 0.73±0.10,1.36±0.20,1.11±0.17, respectively. Compared with the sham group, the number of escape reaction, the number of normal nerve cells in CA1 area, the ratio of LC3-Ⅱ/LC3-Ⅰand the level of p-PI3K/PI3K protein in the SAH group and the Fas group decreased or reduced (P<0.05), the escape reaction time and the levels of p62 protein and p-mTOR/mTOR protein in CA1 area increased or promoted (P<0.05), However, the number of escape reaction, the number of normal nerve cells in CA1 area, the ratio of LC3-Ⅱ/LC3-Ⅰand the level of p-PI3K/PI3K protein in the fasudil group were higher or more than those in the SAH group (P<0.05), and the escape reaction time and the levels of p62 protein and p-mTOR/mTOR protein in CA1 area were lower or lower than those in the SAH group (P<0.05). 【Conclusion】Fasudil can enhance the autophagy of neurons in hippocampal CA1 region of SAH rats, protect cells, and then enhance the learning and memory function of rats. The mechanism may be realized by inhibiting the activation of PI3K/mTOR pathway. 2022 Vol. 39 (1): 18-22 [Abstract] ( 65 ) HTML (1 KB)  PDF (1669 KB)  ( 66 ) 23 Effect of Postoperative Fluid Balance on Prognosis of Patients with Severe Preeclampsia FENG Li-yuan, WANG Zhen-hui DOI: 10.3969/j.issn.1671-7171.2022.01.006 【Objective】To retrospectively analyze the effects of different fluid balance on the prognosis and hospital stay of patients with severe preeclampsia. 【Methods】A total of 683 patients with severe preeclampsia who underwent cesarean section in our hospital was selected. The water inflow and outflow for 24 hours were recorded in the first 3 days after operation. They were divided into three groups according to different fluid balance after operation. Group A was positive fluid balance (n=134, fluid inflow and outflow within 3 days after operation >200 mL), and group B was fluid balance (n=189, fluid inflow and outflow within 3 days after operation -200~200 mL), Group C was the negative fluid balance group (n=360, the amount of fluid in and out within 3 days after operation was ≤-1000 mL). The heart rate, blood oxygen saturation, maximum systolic blood pressure, maximum diastolic blood pressure, brain natriuretic peptide (BNP), urinary specific gravity and the length of hospital stay in each group were compared 3 days after operation, and the negative rate of urinary protein, blood pressure and BNP return to normal 42 days after delivery were compared. 【Results】The blood oxygen saturation of the group A and the group B was lower than that of the group C (P<0.05), but there was no significant difference between the group A and the group B (P>0.05); The heart rate, maximum systolic blood pressure, maximum diastolic blood pressure and hospital stay in the group A and the group B were significantly higher than those in the group C (P<0.05), but there was no significant difference between the group A and the group B (P>0.05); There was no significant difference in BNP level and urinary specific gravity among the three groups (all P>0.05). At 42 days postpartum, the negative rate of urinary protein in group A was lower than that in the group C (P<0.05), and the return to normal rate of blood pressure in the group A was lower than that in the group B and the group C (P<0.05), but there was no significant difference between the group B and the group C (P>0.05); BNP returned to normal in the three groups, and there was no significant difference (P>0.05). 【Conclusion】The negative balance of fluid inflow and outflow after severe preeclampsia can improve the patient's blood pressure, increase the blood oxygen saturation, reduce the length of hospital stay, and effectively improve the patient's prognosis. 2022 Vol. 39 (1): 23-25 [Abstract] ( 63 ) HTML (1 KB)  PDF (1145 KB)  ( 34 ) 26 Comparison of Therapeutic Effects on Endoscopic Discectomy Through Intervertebral Foramen via Interbody Approach and Open Surgery in the Treatment of Lumbar Disc Herniation ZHANG Lin-lin, QIN Guo-liang DOI: 10.3969/j.issn.1671-7171.2022.01.007 【Objective】To compare endoscopic discectomy through intervertebral foramen via interbody approach (PTED) and open surgery in the treatment of lumbar disc herniation. 【Methods】A total of 80 patients with lumbar disc herniation treated in our hospital from January 2014 to December 2018 were divided into two groups according to different surgical methods, with 40 cases in each group. The control group was given traditional open laminectomy and the observation group was given vertebral pted surgery. The clinical effects of the two groups were compared.【Results】The intraoperative blood loss in the observation group was (38.63±8.12) mL, less than (102.52±18.66) mL in the control group, the incision length was (0.90±0.23) cm, shorter than (4.12±0.85) cm in the control group, the difference was statistically significant (P<0.05). There was no significant difference in the levels of interleukin-17 (IL-17) and 5-hydroxytryptamine (5-HT) (P>0.05). The levels of IL-17 and 5-HT in the control group were lower than those in the control group, The difference was statistically significant (P<0.05). There was no significant difference in preoperative visual analogue (VAS) scores between the two groups (P>0.05). The VAS scores of the observation group were significantly lower than those of the control group at 3 days, discharge and 6 months after operation (P<0.05). All patients were followed up. The excellent and good rate in the observation group was 90.00% at 6 months after operation, which was significantly higher than 72.50% in the control group (P<0.05). 【Conclusion】Compared with open surgery, pted has the advantages of less trauma, rapid recovery and less pain, and is worthy of clinical application. 2022 Vol. 39 (1): 26-29 [Abstract] ( 61 ) HTML (1 KB)  PDF (1170 KB)  ( 83 ) 30 Effect of One Side Vidian Neurectomy Combined with Another Side Selective Vidian Neurectomy in Treatment of Allergic Rhinitis LI Xiang-sheng, LIANG Dan, LIU Tian, et al DOI: 10.3969/j.issn.1671-7171.2022.01.008 【Objective】To investigate the effect of one side vidian neurectomy combined with selective vidian neurectomy on the side in the treatment of allergic rhinitis and its influence on tear secretion.【Methods】A total of forty-four patients with allergic rhinitis were treated with vidian neurectomy on one side and selective vidian neurectomy on the other side. Nasal symptom visual analogue scale and test Schirmer I were compared before and after surgery to observe the treatment effect and complications. 【Results】Among the 44 patients, 27 cases (61.4%) were markedly effective, 16 cases (36.3%) were effective, and 1 case (2.3%) was ineffective. The overall effective rate was 97.7% (43/44). Postoperative scores of VAS at the time of 6 and 12 months were lower than preoperative scores and the difference was statistically significant (P<0.05). On the 5th day, 1 month, and 3 months after surgery, the average level of tear secretion in the left eye was lower than that before surgery (P<0.05). On the 5th day and 1 month after surgery, the average level of tear secretion in the right eye was lower than that before surgery, the difference was statistically significant (P<0.05). Three months after operation, there was no significant difference in the average level of right tear secretion compared with that before operation (P>0.05). On the 5th day after operation, 1 month and 3 months after operation, the average level of left tear secretion was significantly lower than that of the right eye, and the differences were all significant (P<0.05).【Conclusion】Endoscopic vidian neurectomy combined with Elective vidian neurectomy has a good short-term effect in the treatment of allergic rhinitis, and has little effect on postoperative tear secretion. 2022 Vol. 39 (1): 30-32 [Abstract] ( 61 ) HTML (1 KB)  PDF (1142 KB)  ( 88 ) 33 Effects of Super Long Effect Amlodipine Subcutaneous Implant on Liver and Kidney Function in Spontaneously Hypertensive Rats WANG Wen-feng, LUO Yu-mei, ZENG Gui-rong, et al DOI: 10.3969/j.issn.1671-7171.2022.01.009 【Objective】To investigate the effect of subcutaneous implantation of super long-acting amlodipine on liver and renal function in spontaneously hypertensive rats (SHR). 【Methods】Organic polymer polycaprolactone was synthesized into sustained-release carrier, and amlodipine sustained-release preparation which can be implanted subcutaneously was prepared by adding amlodipine raw material. SHR was randomly divided into model control group (MC group), blank carrier implantation group (BCE group), amlodipine solution gavage group (ASG group) and amlodipine sustained-release preparation implantation group (ARI group), with 8 rats in each group; The normal control group (NC group) was 8 WKY rats. SHR in the NC group and the MC group were not intervened. Blank carrier and amlodipine sustained-release agent were implanted into the cervical dorsal subcutaneous layer of SHR in the BCE group and the Ari group respectively. SHR in the ASG group was given amlodipine solution (0.45 mg/kg) by gavage for 12 weeks. During the experiment, the body weight and tail artery blood pressure of rats were measured regularly, and venous blood was collected to detect liver and kidney functions. 【Results】After 12 weeks of intervention, the systolic blood pressure, diastolic blood pressure and mean arterial pressure of SHR in the ASG group and the ARI group were lower than those in the MC group (P<0.01), and there was no significant difference between the ASG group and the NC group (P>0.05). Compared with the MC group and the ASG group, the serum aspartate aminotransferase (AST) of SHR in the ARI group decreased, but the difference was not statistically significant (P=0.054); Serum creatinine (CR) in SHR increased in all groups, but there was no significant difference compared with the NC group (P>0.05). 【Conclusion】Subcutaneous implantation of super long-acting amlodipine sustained-release preparation may have a protective effect on liver function while steadily reducing blood pressure in SHR, and its effect on renal function is not significantly different from that of oral drugs. 2022 Vol. 39 (1): 33-36 [Abstract] ( 79 ) HTML (1 KB)  PDF (1727 KB)  ( 115 ) 37 Application of Extrathecal Dissection of Liver Pedicle Guided by Venous Ligament in Laparoscopic Left Hemihepatectomy GAO Qing-dong, WANG Xiao-lin, BAI Wei-bing DOI: 10.3969/j.issn.1671-7171.2022.01.010 【Objective】To explore the application value of hepatic pedicle extrathecal dissection guided by venous ligament in laparoscopic left hemihepatectomy. 【Methods】A total of 70 patients requiring liver resection in our hospital from January 2018 to January 2020 were divided into the control group and the observation group according to the surgical plan, with 35 cases in each group. The control group underwent conventional intrathecal anatomy of the liver pedicle, and the observation group underwent extrathecal anatomy of the liver pedicle under the guidance of venous ligament. The clinical efficacy and clinical indicators of the two groups were compared and the changes of liver function before and after treatment were analyzed. and Incidence of complications in the two groups were compared as well. 【Results】The total effective rate of the observation group was 94.30%, which was not statistically different from the 88.60% of the control group (P>0.05). There was no statistically significant difference between the two groups of postoperative drainage tube removal, gastrointestinal function recovery and hospitalization time (P>0.05).The operation time, left liver pedicle dissection time and intraoperative blood loss in the observation group were lower than those in the control group (P<0.05).After treatment, the levels of ALT, AST and TBIL in the two groups were higher than before treatment (P<0.05), while the level of ALB was lower than before treatment (P<0.05). ALT and AST levels in the observation group after treatment were lower than those in the control group (P<0.05). There was no significant difference in the levels of ALB and TBIL compared with the control group (P>0.05). The incidence of complications in the observation group was 8.55%, which was not statistically different from 11.40% in the control group (P>0.05). 【Conclusion】In the laparoscopic-assisted left hemihepatectomy, the transvenous ligament-guided hepatic pedicle extrathecal anatomy has a significant effect, which reduces intraoperative injuries. It is worthy of promotion. 2022 Vol. 39 (1): 37-40 [Abstract] ( 55 ) HTML (1 KB)  PDF (1164 KB)  ( 55 ) 41 Correlation between Soluble Growth Stimulating Factor 2 and Left Ventricular Remodeling after Acute Myocardial Infarction RUAN Wen-ting, WEI Xi-jin, GUAN Lin DOI: 10.3969/j.issn.1671-7171.2022.01.011 【Objective】To investigate the correlation between soluble growth stimulating expression gene 2 (sST2) and left ventricular remodeling after acute myocardial infarction (AMI). 【Methods】A total of 126 patients with AMI treated in our hospital from March 2019 to March 2020 was selected. According to the echocardiographic results of the sixth month after discharge, they were divided into observation group (left ventricular remodeling,n= 31) and control group (no left ventricular remodeling,n= 95). Blood lipid, myocardial injury markers and serum ST2 levels were compared between the two groups. The clinical value of sST2 in the diagnosis of left ventricular remodeling in patients with AMI after 6 months was evaluated by receiver operating characteristic (ROC) curve. 【Results】There was no significant difference in the levels of blood lipid and creatine kinase isoenzyme (CK-MB) between the two groups (P>0.05); The levels of N-terminal pro brain natriuretic peptide (NT-proBNP), troponin I (cTnI) and sST2 in the observation group were significantly higher than those in the control group (P<0.05). Six months after discharge, the level of left ventricular end diastolic volume (LVEDV) in the observation group was higher than that in the control group for one week, and the levels of LVEDV and △LVEDV in the observation group were higher than those in the control group (P<0.05). Pearson correlation analysis showed that sST2 was positively correlated with cTnI, LVEDV and △LVEDV (r=0.419, 0.517, 0.642, mean P<0.05), and negatively correlated with left ventricular ejection fraction (LVEF) (r=-0.365, P<0.05). ROC curve analysis showed that the area under the value curve (AUC) (95%CI) of sST2 in the diagnosis of left ventricular remodeling in patients with AMI was 0.79 (0.71~0.87), and the best diagnostic limit was 9.86 ng/mL. 【Conclusion】The level of sST2 in patients with left ventricular remodeling after AMI is significantly increased. sST2 is a potential index to evaluate left ventricular remodeling. 2022 Vol. 39 (1): 41-43 [Abstract] ( 60 ) HTML (1 KB)  PDF (1234 KB)  ( 60 ) 44 Clinical Significance of HER-2 Expression Detection by PCR in Axillary Lymph Nodes of StageⅠBreast Cancer with HER-2-postive FAN Zhou, FAN Pei-zhi DOI: 10.3969/j.issn.1671-7171.2022.01.012 【Objective】Polymerase chain reaction (PCR) can improve the detection rate of micrometastasis. To detect HER-2 expression by PCR in axillary lymph nodes of stage Ⅰ HER-2-postive breast cancer and study its clinical significance. 【Methods】The clinical and pathological data of 128 cases of stage Ⅰ breast cancer with HER-2-postive expression were collected. HER-2 gene expression levels in axillary lymph nodes were detected by PCR. The clinicopathological characteristics, treatment and prognosis were retrospectively analyzed by using the statistical methods such as Kaplan-Meier. 【Results】The pathological type of 128 cases was infiltrative non-specific carcinoma with HER-2 positive expression and negative expression of ER and PR. During follow-up, 3 patients had local recurrence, 13 had distant metastases, and 10 patients had distant metastases who died during follow-up. DFS (disease-free survival) rate was 89.1% (114/128). PCR detection of HER-2 showed that 42 cases were positive expression and 86 cases were negative expression in axillary lymph nodes. The clinicopathological characteristics were not statistically different between the two groups (P>0.05). DFS rate was 78.6% (33/42) in HER-2 positive expression group and 94.2% (81/86) in HER-2 negative expression group, respectively; The difference of DFS rate was statistically significant (P<0.05). In HER-2 positive expression group, 27 patients received trastuzumab treatment and the DFS rate was 92.6% (25/27), while the DFS of 15 patients without receiving trastuzumab was 53.3% (8/15). The difference in DFS between the two groups was statistically significant (P<0.05). In HER-2 negative expression groups, DFS rate was 96.6% (56/58) in 58 cases who received trastuzumab and 89.3% (25/28) in 28 cases without receiving trastuzumab. The DFS rate of two groups had no significant difference (P>0.05). 【Conclusion】Overexpression of HER-2 implies the indication of axillary lymph node micrometastasis. Breast cancer patients with HER-2 positive expression in axillary lymph node have poor prognosis. Trastuzumab can improve the prognosis of patients with micrometastasis of axillary lymph nodes. 2022 Vol. 39 (1): 44-47 [Abstract] ( 52 ) HTML (1 KB)  PDF (1361 KB)  ( 40 ) 48 Clinical Observation of Internal and External Head Approach through Popliteal Gastrocnemius in the Treatment of Tibial Plateau Fractures Involving Posterior Column LUO Xin-gang, GAO Chang-cheng DOI: 10.3969/j.issn.1671-7171.2022.01.013 【Objective】To investigate the clinical effect of popliteal gastrocnemius internal and external head approach in the treatment of elderly patients with tibial plateau fractures involving the posterior column. 【Methods】A total of 98 elderly patients with tibial plateau fractures involving the posterior column from January 2012 to January 2020 was randomly divided into observation group and control group, with 49 cases in each group. The observation group was treated by internal and external head approach through popliteal gastrocnemius muscle, and the control group was treated by standard anterolateral approach. The tibial plateau varus angle and caster angle were measured by imaging examination. 12 months after operation, the curative effect was evaluated by Rasmussen radiology score, and the knee function and stability were evaluated by New York Special Surgery Hospital scoring system (HSS). The therapeutic effects of the two groups were compared. 【Results】There was no significant difference in average operation time and average intraoperative bleeding between the two groups (P>0.05). The postoperative hospital stay and fracture healing time in the observation group were shorter than those in the control group (P<0.05); There was no significant difference in tibial plateau varus angle and retroversion angle between the two groups immediately after operation, 1 month, 3 months, 6 months and 12 months after operation (P>0.05). The Rasmussen score of the observation group was (15.72±2.26), and the excellent and good rate was 100%. The Rasmussen score of the control group was (14.05±1.98), and the excellent and good rate was 91.84% (45/49). The Rasmussen score and excellent and good rate of the observation group were higher than those of the control group (P<0.05). 12 months after operation, the HSS score and knee range of motion in the observation group were higher than those in the control group (P<0.05); In the observation group, only one patient developed infection one month after operation and returned to normal after treatment. In the control group, one patient had joint stiffness and relieved after targeted rehabilitation training.【Conclusion】The internal and external head approach through popliteal gastrocnemius muscle is a safe and reliable method for the treatment of elderly patients with tibial plateau fractures involving the posterior column. The postoperative fracture healing is good and the knee function returns to normal. 2022 Vol. 39 (1): 48-51 [Abstract] ( 60 ) HTML (1 KB)  PDF (1168 KB)  ( 75 ) 52 Effect of Serum Uric Acid Level During Pregnancy on Congenital Heart Disease in Children CHEN Li, SONG Yan-qin DOI: 10.3969/j.issn.1671-7171.2022.01.014 【Objective】To investigate the effect of serum uric acid level during pregnancy on children with congenital heart disease (CHD). 【Methods】A total of 20 pregnant women who gave birth to CHD children in the obstetrics of our hospital were selected as the observation group, and 100 pregnant women whose offspring did not suffer from CHD in the same period were selected as the control group. The levels of serum uric acid and related indexes were compared between the two groups. The relationship between maternal indexes and offspring CHD was analyzed by binary logistic regression. 【Results】There was no significant difference in average age, gestational weeks, times of delivery and body mass index (BMI) between the two groups (P>0.05). The serum uric acid level, second-hand smoke exposure rate, abortion history and prevalence of hyperuricemia in the observation group were significantly higher than those in the control group, and there were significant differences between the two groups (P<0.05). Univariate logistic regression analysis showed that maternal BMI, second-hand smoke exposure, abortion history and hyperuricemia were correlated with the risk of CHD in offspring (P<0.05), but not with age, gestational week, gestational times and delivery times (P>0.05). The significant indexes of univariate analysis were included in multivariate logistic regression analysis. The results showed that maternal BMI, second-hand smoke exposure, abortion history and hyperuricemia were independent risk factors for CHD in offspring (P<0.05). 【Conclusion】Elevated serum uric acid level is a risk factor for CHD in children. Active treatment of hyperuricemia in pregnant women may reduce the incidence rate of CHD in children. 2022 Vol. 39 (1): 52-54 [Abstract] ( 64 ) HTML (1 KB)  PDF (1145 KB)  ( 109 ) 55 Effects of Dodder Extract-containing Serum on Apoptosis and Secretory Function of Isolated Mouse Ovarian Granulosa Cells ZHANG Jing-jing, XUE Bao-yao, ZHANG Ling-yan DOI: 10.3969/j.issn.1671-7171.2022.01.015 【Objective】To investigate the effects of serum containing dodder extract (semen cuscutae)-on apoptosis and secretion of isolated mouse ovarian granulosa cells in vitro. 【Methods】A total of 18 SPF-grade SD rats were divided into three groups (n=6). Rats in the low and high dose of dodder extract groups were intragastric administration of 893.77 mg/kg and 4468.55 mg/kg of Cuscuta Chinensis (semen cuscutae) for 3 consecutive days, respectively. Then serum with dodder extract and blank serum were obtained from abdominal aorta. Ten 3-week-old SPF SD female mice were intraperitoneally injected with pregnant equine serum gonadotropin 5 IU. Thereafter ovaries were isolated, granulosa cells were identified and cultured in vitro. The granulosa cells were further served as control group (cultured medium containing blank serum alone), ZEA group(medium containing 60 μmol/L ZEA and blank serum), low-dose and high-dose drug-containing serum group (medium containing 60 μmol/L ZEA plus low-dose and high-dose dodder extract-containing serum, respectively). The proliferation and apoptosis of granulosa cells were detected by CCK-8 and Annexinv/PITC staining and levels of Estradiol (E2) and progesterone (P) were detected by radioimmunoassay. Levels of PCNA, Bax-2 and Bcl-2 related x protein (Bax) were detected by immunoblot.【Results】Compared with the control group, the ovarian granulosa cell proliferation inhibition rate, apoptosis rate, Bax protein level increased (P<0.05), E2、P PCNA and Bcl-2 levels decreased(P<0.05) in ZEA group. Compared to ZEA group, the ovarian granulosa cell proliferation inhibition rate, apoptosis rate, Bax protein level in low and high dose of dodder extract-containing serum group decreased (P<0.05),while levels of E2,P,PCNA and Bcl-2 increased (P<0.05). The ovarian granulosa cell proliferation inhibition rate, apoptosis rate, Bax protein level in the high-dose dodder extract-containing serum group decreased more than those in the low-dose group(P<0.05), and levels of E2,P,PCNA andBcl-2 increased more than those in the low-dose group (P<0.05). 【Conclusion】The serum of dodder extract (semen cuscutae) can inhibit the apoptosis of ovarian granulosa cells induced by ZEA and improve the secretory function of ovarian granulosa cells. 2022 Vol. 39 (1): 55-58 [Abstract] ( 60 ) HTML (1 KB)  PDF (1328 KB)  ( 120 ) 59 Effects of Reteplase Combined with Bivalirudin on TIMI Blood Flow Grade and MACE Incidence after PCI for Acute Myocardial Infarction MA Xiao-lei, SHI Yue-fei DOI: 10.3969/j.issn.1671-7171.2022.01.016 【Objective】To investigate the effects of reteplase combined with bivalirudin on the blood flow grade of myocardial infarction thrombolysis test (TIMI) and the occurrence of major adverse cardiovascular events (MACE) after percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI). 【Methods】A total of 114 AMI patients who underwent PCI in our hospital was prospectively selected and randomly divided into observation group (Reteplase combined with bivalirudin) and control group (Reteplase treatment), 57 cases in each group. TIMI blood flow grade, cardiac function indexes [left ventricular end diastolic volume (LVEDV), left ventricular end systolic volume (LVESV), vascular resistance (SVR), left ventricular ejection fraction (LVEF), serum vascular endothelial function indexes nitric oxide (NO), nitric oxide synthase (NOS), endothelin (ET-1)],serum myocardial injury indexes [lactate dehydrogenase (LDH), troponin (cTNI) and creatine kinase isoenzyme (CK-MB)] were compared between the two groups. The incidence of MACE at 1 month after operation was recorded. 【Results】One week after operation, the proportion of TIMI blood flow grade 3 and LVEF level in the observation group were higher than those in the control group, and the levels of LVEDV, LVESV and SVR in the observation group were lower than those in the control group (P<0.05); One week after operation, the levels of serum LDH, cTNI, CK-MB and ET-1 in the observation group were lower than those in the control group, the levels of serum NO and NOS in the observation group were higher than those in the control group (P<0.05). The incidence of mace in the observation group one month after operation was lower than that in the control group, the difference was statistically significant (P<0.05). 【Conclusion】Reteplase combined with bivalirudin can significantly improve myocardial perfusion, cardiac function and vascular endothelial function, reduce myocardial injury and effectively reduce the incidence of MACE in patients with AMI after PCI. 2022 Vol. 39 (1): 59-62 [Abstract] ( 56 ) HTML (1 KB)  PDF (1167 KB)  ( 54 ) 63 Analysis of the Value of Age, LH/FSH, and AMH in Predicting the Clinical Outcome of in Vitro Fertilization-Embryo Transfer CAO Yang-yang, CHANG Wang-yan DOI: 10.3969/j.issn.1671-7171.2022.01.017 【Objective】To analyze the value of age, luteinizing hormone/basal follicle stimulating hormone (LH/FSH), and anti-Müllerian hormone (AMH) in predicting the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET). 【Methods】A total of 186 patients (214 cycles) who underwent IVF-ET and had pregnancy outcomes in our hospital from February 2018 to December 2020 were selected as the research objects. Statistics of pregnancy outcome were divided into the pregnancy success group and pregnancy failure group according to whether the pregnancy was successful or not. the clinical data were compared between the successful pregnancy group and the pregnancy failure group. Logistic regression analysis was explored the relevant factors that affected the clinical outcome of IVF-ET assisted pregnancy. A receiver operating characteristic curve (ROC) was made, and the area under the curve (AUC) was used to analyze the predictive value of age, LH/FSH, AMH and the combination of the three on the clinical outcome of IVF-ET assisted pregnancy. 【Results】In this study, there were 76 successful pregnancy cases, the cycle clinical pregnancy rate was 40.86% and the clinical pregnancy rate was 35.51% (76/186). The pregnancy failure group's age, human gene recombinant follicle stimulating hormone (Gn) dosage, and FSH were all higher than the pregnancy success group (P<0.05), and the pregnancy failure group had lower embryo number, antral follicle number (AFC), LH/FSH, and AMH compared to the successful pregnancy group (P<0.05). Logistic multivariate analysis showed that age, number of embryos transferred, LH/FSH and AMH were the related factors affecting the clinical pregnancy outcome of IVF-ET assisted women (OR=3.522, 2.852, 3.770, 4.108, P<0.05). ROC analysis showed that the best cut-off points of age, LH/FSH, and AMH for predicting the clinical outcome of IVF-ET assisted fertility were 31.96 years, 1.79 μg/L and 3.28 μg/L, respectively. The specificity of the combination of the three was 98.39%, which was higher than the specificity evaluated by age, LH/FSH and AMH alone. The AUC predicted by the combination of age, LH/FSH, and AMH for clinical outcome of IVF-ET assisted fertility was 0.896, which was significantly higher than the AUC predicted by age, LH/FSH and AMH alone (P<0.05). 【Conclusion】The combination of age, LH/FSH and AMH has a high value in predicting the clinical outcome of IVF-ET, and it is suitable as an important reference index for clinical prediction of the clinical outcome of IVF-ET assisted pregnancy. 2022 Vol. 39 (1): 63-66 [Abstract] ( 68 ) HTML (1 KB)  PDF (1260 KB)  ( 88 ) 67 Clinical Analysis of Transurethral Plasma Enucleation of Prostate in the Treatment of Benign Prostatic Hyperplasia with Prostate Volume >80 mL TANG Yao, CHEN Guang-yu, FU Chong-de, et al DOI: 10.3969/j.issn.1671-7171.2022.01.018 【Objective】To investigate the effect of transurethral plasma enucleation of prostate in the treatment of benign prostatic hyperplasia (BPH) with prostate volume >80 mL. 【Methods】A total of 128 BPH patients with prostate volume >80 mL was analyzed retrospectively. They were divided into observation group (n=66) and control group (n=62). The control group was treated with transurethral bipolar resection of the prostate and the observation group was treated with transurethral plasma enucleation of the prostate. The operation conditions and surgical stress [serum interleukin (IL)-6 and C-reactive protein (CRP) before and 24 hours after operation], urodynamics [maximum urinary flow rate (Qmax), residual urine volume (RUV), maximum detrusor pressure (Pdet)] and complications before and 3 months after operation were compared between the two groups.【Results】The intraoperative blood loss, bladder flushing time and hospital stay in the observation group were less than those in the control group, and the quality of lesion resection was greater than that in the control group (P<0.05). 24 hours after operation, the levels of serum IL-6 and CRP in the two groups were higher than those before operation, and the levels in the observation group were lower than those in the control group (P<0.05). Three months after operation, the Qmax of the two groups was higher than that before operation, RUV and Pdet were lower than that before operation, and the difference between the observation group and the control group was statistically significant (P<0.05). The incidence of complications in the observation group was 3.03% (2/66), lower than 6.45% (4/62) in the control group, but the difference was not statistically significant(χ2=0.247,P=0.619). 【Conclusion】Transurethral plasma enucleation of the prostate is effective in the treatment of BPH with prostate volume >80 mL, it can reduce body stress, improve urodynamics, have less complications and high safety. 2022 Vol. 39 (1): 67-69 [Abstract] ( 45 ) HTML (1 KB)  PDF (1145 KB)  ( 56 ) 70 Risk Factors of Urosepsis after Urinary Stone Surgery and Efficacy of Early Continuous Renal Replacement Therapy GAO Peng, WANG Peng, WU Zhe DOI: 10.3969/j.issn.1671-7171.2022.01.019 【Objective】To investigate the risk factors of urosepsis after urinary stone surgery and discuss the efficacy of early continuous renal replacement therapy for postoperative urosepsis. 【Methods】Of 2752 patients with urinary stone surgery in the Department of Urology Surgery of our hospital,167 patients with urosepsis were in the urosepsis group, and the remaining 2585 patients without urosepsis were in the non-urosepsisl group. Multivariate Logistic regression analysis was used to screen the risk factors affecting urosepsis. Thereafter, patients in the urosepsis group were further divided into two subgroups according to different treatment methods:67 patients with early continuous renal replacement therapy were classified into the observation group and 100 patients without early continuous renal replacement therapy were set as control group. The changes of relevant serological indexes after treatment were compared between two groups. 【Results】The incidence of postoperative urosepsis in patients was correlated with the age, preoperative antibiotic use, blood routine test, urine routine test, urine culture, stone size, operation time and perfusion volume (P<0.05). Age ≥60 years, positive blood routine test, positive urine routine test, positive urine culture, stone size >2.0 cm, operation time >100 min and intraoperative perfusion volume >30 L were all risk factors for urosepsis after urinary stone surgery. At 48h after operation, levels in procalcitonin, C-reactive protein, white blood cell count and neutrophils ratio in the observation group was significantly lower than those in the control group (P<0.05). While the levels of hemoglobin, platelet count, fibrinogen and standards bicarbonate in observation group was also significantly higher than that in control group (P<0.05). 【Conclusion】Age ≥ 60 years old, blood routine test (+), urine routine test (+), urine culture (+), stone size >2.0 cm, operation time >100 min and perfusion volume > 30 L are independent risk factors for patients with urosepsis after urinary stone surgery. In this circumstance, early continuous renal replacement therapy can effectively improve the prognosis of patients with urosepsis after urinary stone surgery. 2022 Vol. 39 (1): 70-73 [Abstract] ( 58 ) HTML (1 KB)  PDF (1165 KB)  ( 52 ) 74 Effect of Simiao Decoction on Serum Inflammatory Factors and NALP3 Expression in Patients with Acute Gout Attack WANG Miao, PEI Yi-ming DOI: 10.3969/j.issn.1671-7171.2022.01.020 【Objective】To investigate the effect of Simiao Decoction on the expression level of inflammatory cytokines and nucleotide binding oligomerization domain-like receptor 3 (NALP3) in patients with acute attack of gout. 【Methods】A total of 102 patients with acute onset of gouty arthritis, who were diagnosed in the inpatient department of our hospital from February 2019 to January 2020, were selected and divided into the observation group and the control group according to the random number table method, with 51 patients in each group. Patients in the control group were treated with colchicine, while patients in the observation group were additionally treated with supplemented Simiao decoction (1 dose per day). Both groups were treated for 14 days continuously. The levels of interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1 (IL-1) and NALP3 before and after treatment were compared between the two groups. 【Results】The total effective rate in the observation group was 92.16% (47/51), which was significantly higher than that of 74.51% (38/51) in the control group (P<0.05).After treatment, the levels of IL-6, IL-8, IL-1, serum uric acid and NALP3 in the observation group were lower than those in the control group and before treatment (P<0.05).The scores of TCM syndromes and local joint symptoms in the observation group were lower than those in the control group and before treatment (P<0.05).There was no statistically significant difference in the incidence of adverse reactions after treatment between the two groups (P>0.05). 【Conclusion】The treatment of acute gout patients with flavored Simiao decoction can effectively relieve joint symptoms, reduce inflammatory response and uric acid level, and inhibit the activation of serum NALP3 inflammatory body, which is of certain clinical significance. 2022 Vol. 39 (1): 74-76 [Abstract] ( 55 ) HTML (1 KB)  PDF (1142 KB)  ( 51 ) 77 Effect of B-ultrasound Assisted Mammotome Minimally Invasive Breast Resection on Serum Inflammatory and Pain Factors in Elderly Patients with Benign Breast Tumors LIU Chun, LIU Yun-chong, YU Zhen-bin, et al DOI: 10.3969/j.issn.1671-7171.2022.01.021 【Objective】To explore the effect of B-ultrasound assisted Mammotome minimally invasive breast resection on postoperative recovery and serum pain factors,expression in elderly patients with benign breast tumors. 【Methods】The clinical data of 92 elderly patients with benign breast tumors in Jinan Maternal and Child Care Hospital were selected for retrospective analysis. They were divided into the observation group (n=47) and the control group (n=45) according to different treatment methods. The control group underwent open surgery, while the observation group underwent B-ultrasound assisted Mammotome minimally invasive breast resection. The surgical status, complications, postoperative tumor residual status, treatment satisfaction, and incision aesthetic score, serum pain factors and immune function indexes before operation and after operation were compared between the two groups. 【Results】The operation time, incision healing time, hospitalization time and scar length in the observation group were shorter than those in the control group, and the intraoperative blood loss was lower than that of the control group (P<0.05). Serum levels of NPY, 5-HT, PGE2, IL-10, and CRP in the two groups before surgery were higher than those on the 3rd day after surgery, but the observation group was lower than the control group and the difference was statistically significant (P<0.05). The levels of serum IL-10 and CRP in the two groups were higher than those before operation, and the observation group was lower than the control group. The levels of INF-γ in the two groups were lower than those before operation, and the observation group was higher than the control group (P<0.05). There were 2 cases (4.26%) of residual tumor in the observation group, which was lower than 3 cases (6.67%) in the control group, and the difference was not statistically significant (P>0.05). The incidence of complications in the observation group was 6.38% (3/47), which was lower than 22.22% (10/45) in the control group, and the difference was statistically significant (P＜0.05). 【Conclusion】The use of B-ultrasound assisted Mammotome minimally invasive breast resection for the treatment of the elderly patients with benign breast tumors has the advantages of short operation time, small scars, light pain, quick postoperative recovery, less trauma, fewer complications, high incision aesthetics and better patient satisfaction. Also it can reduce the body's inflammatory response and immunosuppressive state, which provides significant curative effect and fewer complications. 2022 Vol. 39 (1): 77-80 [Abstract] ( 67 ) HTML (1 KB)  PDF (1171 KB)  ( 141 ) 81 Effect of Estradiol Tablets/Didydrogesterone Tablets on Endometrial Thickness and Volume in Patients with Endometrial Infertility WANG Xin-xiang, ZHOU Mi DOI: 10.3969/j.issn.1671-7171.2022.01.022 【Objective】To investigate the effect of estradiol tablets/didrogesterone tablets on endometrial thickness and volume in patients with thin endometrial infertility. 【Methods】A total of 98 cases of endometrial infertility patients admitted to our hospital from November 2019 to November 2020 were selected as the research objects.They were divided into the observation group and the control group by random number table method. The control group was given estradiol valerate tablets, while the observation group was treated with estradiol tablets/didrogesterone tablets. The endometrial thickness, endometrial volume, estradiol (E2), luteinizing hormone (LH), progesterone levels, adverse reactions during treatment, pregnancy rate and pregnancy outcome within one year were compared between the two groups before and after treatment. 【Results】The endometrial thickness in both groups was significantly increased after treatment (P<0.05). More important evidence is that the endometrial thickness in the observation group was significantly higher than that in the control group after treatment. The time for endometrial thickness to reach 8mm was significantly shorter than that in the control group with statistical significance (P<0.05). The proportion of endometrial volume ≥2 mL after treatment in the observation group was significantly higher than that in the control group, and the difference was statistically significant (P<0.05). However, there was no statistically significant difference in endometrial type between the two groups before and after treatment (P>0.05). After treatment, levels of E2 and progesterone in both groups increased significantly, while LH level decreased significantly (P<0.05). After treatment, E2 and progesterone levels in the observation group were significantly higher than those in the control group, while LH levels were significantly lower than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). The pregnancy rate of 1-year after treatment in the observation group was significantly higher than that in the control group, and the difference was statistically significant (P<0.05), while there was no statistically significant difference in pregnancy outcomes (early abortion rate and term delivery rate) between the two groups (P>0.05). 【Conclusion】Treatment with estradiol tablets/estradiol dydrogesterone tablets in patients with thin endometrial infertility can significantly improve endometrial thickness and volume to enhance the pregnant rate. 2022 Vol. 39 (1): 81-84 [Abstract] ( 72 ) HTML (1 KB)  PDF (1162 KB)  ( 99 ) 85 Effects of Low-Molecular-Weight Dextran Combined with Large Amounts of Drinking Water on Serum Inflammatory Factors and Uterine Environment in Pregnant Women with Oligohydramnios SHI Fan, BAI Rui DOI: 10.3969/j.issn.1671-7171.2022.01.023 【Objective】To investigate the effect of low-molecular-weight dextran combined with large amounts of drinking water on serum inflammatory factors and uterine environment in pregnant women with oligohydramnios. 【Methods】A total of 112 pregnant women with oligohydramnios in our hospital from January 2015 to December 2020 were selected. Patients were randomly divided into 4 groups: 28 cases of low molecular dextran combined with large amounts of drinking water group, 28 cases of low molecular dextran group, 28 cases in the large amounts of drinking water group and 28 cases in the control group. The serum inflammatory factors [serum tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), and interleukin-6 (Interleukin-6, IL-6), serum high sensitivity reactive C protein (hs-CRP)], uterine cavity environment [amniotic fluid index (AFI), fetal pulse index (pulse index, PI) and the ratio of the maximum systolic blood flow rate of fetal umbilical artery blood flow to the end-diastolic blood flow rate (systolic/diastolic, S/D)] before treatment and 7 days after treatment in the four groups of pregnant women were detected. 【Results】There were no significant differences in age, gestational age, parity, TNF-α, IL-2, hs-CRP, AFI, PI and S/D among the four groups(P>0.05). Compared with the control group, TNF-α, IL-6, hs-CRP, PI, S/D in the low molecular dextran combined with large amounts of drinking water group were significantly decreased, while AFI was significantly increased (P<0.05). The IL-6, Hs-CRP, PI, S/D in low molecular dextran group decreased significantly and AFI increased significantly if they were compared to the control group (P<0.05). Also, compared with the control group, IL-6, hs-CRP, PI, S/D in the large amounts of drinking water group were decreased significantly and AFI was increased significantly (P<0.05). 【Conclusion】Combining low-molecular-weight dextran with large amounts of drinking water in the treatment of oligohydramnios can improve the uterine cavity environment and serum inflammatory factor levels. 2022 Vol. 39 (1): 85-87 [Abstract] ( 63 ) HTML (1 KB)  PDF (1146 KB)  ( 92 ) 88 Correlation and Influencing Factors between Hp Infection and hBD-2, PG Ⅰ/PG Ⅱ and Th1/Th2 Levels in Children with Peptic Ulcer JIANG Qin, HUI Lei DOI: 10.3969/j.issn.1671-7171.2022.01.024 【Objective】To investigate the relationship between Helicobacter pylori (HP) infection with hBD-2,PG Ⅰ/PG Ⅱ and Th 1/Th2 levels in children with peptic ulcer (PU),and its influencing factors. 【Methods】A total of 84 children with PU treated in our hospital was divided into HP positive group (n=31) and HP negative group (n=53) according to the situation of HP infection. The baseline data, inflammatory score, hBD-2, PG Ⅰ/PG Ⅱ and Th1/Th2 levels of the two groups were compared. The correlation between hBD-2, PG Ⅰ/PG Ⅱ and Th1/Th2 and inflammatory score and HP infection was analyzed to explore the influencing factors of HP infection in children with PU. 【Results】The scores of hBD-2, PG Ⅰ/PG Ⅱ and inflammation in the HP positive group were higher than those in the HP negative group, and Th1/Th2 were lower than those in the HP negative group (P<0.05). HBD-2, PG Ⅰ/PG Ⅱ were positively correlated with the inflammatory score of HP positive children, and Th1/Th2 was negatively correlated with the inflammatory score of HP positive children (P<0.05); HBD-2, PG Ⅰ/PG Ⅱ were positively correlated with HP infection, and Th1/Th2 was negatively correlated with HP infection (P<0.05). The history of gastrointestinal diseases, good personal hygiene, meal sharing, finger sucking, hBD-2, PG Ⅰ/PG Ⅱ and Th1/Th2 were the influencing factors of HP infection in children with PU (P<0.05). 【Conclusion】HP infection in children with PU is closely related to the imbalance of hBD-2, PG Ⅰ/PG Ⅱ and Th1/Th2 levels, as well as personal hygiene and the history of gastrointestinal diseases of caregivers. 2022 Vol. 39 (1): 88-90 [Abstract] ( 59 ) HTML (1 KB)  PDF (1145 KB)  ( 58 ) 91 Value of Combined Detection of Tissue MicroRNA in Predicting 1-year Survival Rate of Cervical Cancer WANG Qiao-ge, TIAN Lu DOI: 10.3969/j.issn.1671-7171.2022.01.025 【Objective】To explore the value of combined detection of MicroRNA in cervical cancer tissues in predicting the 1-year survival rate of cervical cancer. 【Methods】A total of 70 patients with cervical cancer admitted to Northwest Women's and Children's hospital from April 2017 to April 2019 were selected for the study. All selected patients were followed up for one year. The 1-year survival rate of the patients was counted and divided into the death group (n=12) and the survival group (n=58). The age, FIGO stage, pathological grade, lymph node metastasis and tumor size of the two groups were compared. MicroRNA-21 (miR-21), microRNA-143 (miR-143) and microrna-373 (miR-373) were measured immediately after admission. Multivariate Logistic regression analysis was used to investigate the risk factors of cervical cancer death in 1-year survival. 【Results】The proportion of age>50 years old, FIGO Ⅲ and Ⅳ stage, moderately differentiated and poorly differentiated in pathological grade, lymph node metastasis and tumor size larger than 4 cm in the death group was significantly higher than that in survival group. The difference was statistically significant(P<0.05).The Ct value of miR-21 in the dead group was significantly lower than that in the survival group. The difference was statistically significant (P<0.05).The Ct values of miR-143 and miR-373 in the death group were significantly higher than those in the surviving group, and the difference were statistically significant (P<0.05).Age > 50 years, FIGO stage Ⅲ/Ⅳ, low differentiation, lymph node metastasis, tumor size >4 cm, miR-21＜20.10,miR-143＞26.23 and miR-373＞34.55 were risk factors for 1-year death in cervical cancer patients (P<0.05). 【Conclusion】MicroRNA is associated with the prognosis of cervical cancer, which can provide valuable reference,if combined with other clinical information of patients, for the evaluation of 1-year survival rate of cervical cancer. 2022 Vol. 39 (1): 91-94 [Abstract] ( 57 ) HTML (1 KB)  PDF (1184 KB)  ( 34 ) 95 Effects of General Anesthesia with Tracheal Intubation Alone or Combined with Peripheral Nerve Block on Acute and Chronic Pain and Plasma Anti-x Factor Activity in Patients with Knee Joint Replacement XIE Ya-ning, GUO Xiao-wei, CEHN Hai-ni, et al DOI: 10.3969/j.issn.1671-7171.2022.01.026 【Objective】To explore the effects of two different anesthesias (simple tracheal intubation general anesthesia and general anesthesia combined with peripheral nerve block) on postoperative acute and chronic pain and plasma clotting factor X activity in patients undergoing knee arthroplasty. 【Methods】A total of 150 patients undergoing knee arthroplasty in our hospital were randomly divided into two group, with 75 cases in each group. The control group only used tracheal intubation general anesthesia, while the observation group used tracheal intubation general anesthesia combined with peripheral nerve block anesthesia. The anesthesia onset time, recovery time, and hemodynamic index levels of the two groups, postoperative pain and changes in the activity of anticoagulant factor X activity in the body after surgery were compared between the two groups. 【Results】Intraoperative recovery time of the observation group was significantly lower than that of the control group (P<0.05). The systolic blood pressure, diastolic blood pressure, and heart rate levels of the control group were significantly lower than those before the operation (P<0.05), while the hemodynamics of the observation group did not show difference before and after surgery; the difference was not statistically significant (P>0.05). The visual analog pain (VAS) score of the observation group was significantly lower than that of the control group at 6 hours after the operation (P<0.05). The observation group was The activities of anticoagulant factor X activity at 10 minutes, 20 minutes, and 60 minutes after the operation was higher than those of the control group (P<0.05).【Conclusion】General anesthesia combined with peripheral nerve block anesthesia for tracheal intubation can significantly reduce the postoperative pain response of patients, with stable hemodynamics and higher activity of anticoagulant factor Xa.Thus the anesthesia effect is superior. 2022 Vol. 39 (1): 95-97 [Abstract] ( 78 ) HTML (1 KB)  PDF (1142 KB)  ( 99 ) 98 Analysis of Sexual Dysfunction Status and Risk Factors in Perimenopausal Women ZHANG Fu-e, QIN Qiu-yan DOI: 10.3969/j.issn.1671-7171.2022.01.027 【Objective】To investigate the current status of perimenopausal female sexual dysfunction (FSD) and analyze its risk factors. 【Methods】A total of 246 perimenopausal women who visited Baoji City People's hospital from May 2019 to December 2020 were selected as the survey subjects, and a questionnaire survey was conducted. According to the occurrence of FSD in perimenopausal women, they were divided into the FSD occurrence group and the FSD non-occurring group according. Compare the clinical data of patients in the occurrence group and the non-occurrence group. Logistic multivariate regression analysis of factors affecting the occurrence of FSD during perimenopause. 【Results】The incidence of perimenopausal FSD was 30.49% (75/246). The age, body mass index (BMI), the proportion of diabetes, the proportion of relationship disharmony, and the triglyceride (TG) level of the FSD occurrence group were significantly higher than those of the FSD non-occurring group (P<0.05). The estradiol (E2) level in the FSD occurrence group was significantly lower than that in the FSD non-occurring group (P<0.05). Logistic regression analysis showed that high age, high BMI, and low E2 level were the influencing factors of FSD in menopausal women (OR=3.343, 2.863, 3.861, all P<0.05). 【Conclusion】The incidence of FSD in perimenopausal period is relatively high. Old age, high BMI and reduced E2 level are the influencing factors of FSD in menopausal women. 2022 Vol. 39 (1): 98-100 [Abstract] ( 56 ) HTML (1 KB)  PDF (1146 KB)  ( 33 ) 101 Expression and Clinical Significance of MMP-2, MMP-9 and CEACAM-1 in Colon Cancer WANG Gong-li, HUANG Yong-nian DOI: 10.3969/j.issn.1671-7171.2022.01.028 【Objective】To investigate the expression levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and carcinoembryonic antigen-associated cell adhesion molecule-1 (CEACAM-1) in colon cancer tissues and the correlation with clinicopathological factors and prognosis. 【Methods】Sixty colon cancer patients who underwent radical resection of colon cancer in our hospital were enrolled. The samples of cancer tissues, paracancerous tissues and normal colon tissues were obtained and the expression levels of MMP-9, MMP-2 and CEACAM-1 were detected. The correlation of clinicopathological factors and disease-free survival (DFS) with expression of MMP-9, MMP-2 and CEACAM-1 were analyzed. 【Results】The positive rates of MMP-9, MMP-2 and CEACAM-1 expression in cancer tissues were higher than those in adjacent tissues and normal colon tissues(P<0.05), and the positive rates of MMP-9, MMP-2 and CEACAM-1 expression in adjacent tissues were higher than normal colon tissue (P<0.05). The expressions of MMP-9, MMP-2 and CEACAM-1 were correlated with serosal invasion, TNM staging, lymph node metastasis and vascular invasion (P<0.05), and their expressions were not correlated with tumor diameter and tumor differentiation (P>0.05). The patients were followed up for 12-38 months, and 28 patients with recurrence and metastasis were showed up. COX multivariate analysis showed that MMP-9, MMP-2, CEACAM-1, TNM, lymph node metastasis were independent prognostic factors for DFS. 【Conclusion】MMP-9, MMP-2 and CEACAM-1 are highly expressed in colon cancer tissues, and high expression is associated with poor prognosis. 2022 Vol. 39 (1): 101-103 [Abstract] ( 62 ) HTML (1 KB)  PDF (1142 KB)  ( 53 )

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