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Quick Search     Adv Search 2020 Vol. 37, No. 5 Published: 2020-05-30   649 Effect of siRNA Targeting UHRF1 on Proliferation and Apoptosis of HeLa Cells GAO Yan-fang, ZHANG Ming, FAN Ju-xiang, et al DOI: 10.3969/j.issn.1671-7171.2020.05.003 【Objective】 To investigate the effects of ubiquitin-like containing PHD and ring finger 1 (UHRF1) gene silencing by specific small interfering RNA (siRNA) on the proliferation and apoptosis in human cervical cancer HeLa cells in vitro.【Methods】 HeLa cells were randomly divided into three groups: the untreated control group without transfection, the negative control group with scramble siRNA transfection and the silencing group which were transfected with the UHRF1 specific gene-targeted siRNA. The mRNA and protein levels of UHRF1 were detected by RT-PCR and western blot, respectively. The effects of UHRF1 gene silencing on the proliferation and apoptosis were analyzed by conducting CCK-8 assay and flow cytometry with Annexin V-FITC/PI staining. 【Results】 The RT-PCR and western blot results showed the mRNA and protein levels of UHRF1 were significantly decreased in HeLa cells after the transfection of UHRF1 gene-targeted siRNA (P<0.01). The proliferation was inhibited and apoptosis was triggered via silencing UHRF1 gene, and the difference between the silencing group and the untreated control group/ negative control group was statistically significant (P<0.01). However,There was no significant difference between the untreated control group and the negative control group (P>0.05). 【Conclusion】 The expression of UHRF1 gene was efficiently interfered by transfecting the UHRF1-targeted siRNA, which resulted in decreased proliferation and enhanced apoptosis in HeLa cells. 2020 Vol. 37 (5): 649-651 [Abstract] ( 172 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 652 Observation on the Therapeutic Effect of Budesonide Aerosol Inhalation on Bronchopulmonary Dysplasia LI Jiang, CHEN Tie-qiang, WU Min, et al DOI: 10.3969/j.issn.1671-7171.2020.05.004 【Objective】 To investigate the effect of Budesonide aerosol inhalation in the treatment of bronchopulmonary dysplasia (BPD) in premature infants. 【Methods】 The clinical data of 92 cases of BPD in our hospital were analyzed retrospectively. According to the treatment methods, they were divided into 29 cases of budesonide group, 34 cases of dexamethasone group and 29 cases of control group (without glucocorticoid).【Results】 After treatment, BPD scores of the budesonide group and the dexamethasone group were lighter than those of the control group, but there was no significant difference between the budesonide group and the dexamethasone group (P>0.05). Compared with the dexamethasone group and the budesonide group, the time of oxygen delivery and the time of noninvasive mechanical ventilation in the control group were significantly longer (P<0.05), but there was no significant difference in the time of hospitalization and oxygen therapy after discharge in the three groups (P>0.05). There was no significant difference in the incidence of sepsis, patent ductus arteriosus (HsPDA), intraventricular hemorrhage (IVH)≥grade Ⅲ, PVL, necrotizing enterocolitis (NEC), gastrointestinal hemorrhage, pulmonary hypertension (PPHN) and hyperglycemia among the three groups (P>0.05).【Conclusion】 For premature infants who need continuous oxygen use or ventilator use, early (≥ 14 days) use of budesonide atomization inhalation has therapeutic effect on their BPD, which can reduce the time of oxygen use and mechanical ventilation, avoid the use of glucocorticoids in the whole body, and no short-term adverse complications are found, but the long-term prognosis still needs further study. 2020 Vol. 37 (5): 652-654 [Abstract] ( 194 ) HTML (1 KB)  PDF  (0 KB)  ( 8 ) 655 Comparison of Two Methods for Detecting Cryptococcus Neoformans Infection in Cerebrospinal Fluid of Immunosuppression Model Rats LIU Hua, KUANG Xiao-ni, HE Xiang-ling, et al DOI: 10.3969/j.issn.1671-7171.2020.05.005 【Objective】 Real-time fluorescent quantitative PCR (FQ-PCR) and ink staining were used to detect rat cerebrospinal fluid (CSF) in a rat model of Cryptococcus neoformans infection. The sensitivity and specificity of the two methods were compared to provide laboratory basis for clinical application. 【Methods】 Sixty male rats were randomly divided into four groups: group A and group C were injected with cyclophosphamide (150 mg/kg) once on the first day of the experiment, while group B and group D were injected with the same amount of 1 mL saline intraperitoneally. To establish infection model, rats in group A and group B were injected with 1 mL (containing 1×106)of Cryptococcus neoformans suspension in the tail vein on the fourth day of the experiment, while rats in group C and group D received 1 ml saline as negative control. On the 6th day of the experiment, the cerebrospinal fluid of all rats was collected. Then Cryptococcus in cerebrospinal fluid of the four groups was detected by FQ-PCR and stained with ink at the same time. The results from the two methods were compared. 【Results】 The positive detection rate of Cryptococcus neoformans by FQ-PCR was higher than that of ink staining, and the difference was statistically significant (P<0.05). 【Conclusion】 FQ-PCR system is superior to ink staining in detecting Cryptococcus neoformans in cerebrospinal fluid of rats. It can be used for clinical detection of the presence of Cryptococcus neoformans infection in cerebrospinal fluid. 2020 Vol. 37 (5): 655-657 [Abstract] ( 132 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 658 Correlation Analysis between 24-hour Ambulatory Blood Pressure Parameters and the Degree of Coronary Artery Disease XIANG Yi, OU Bai-qing, XIAO Zhi-lin DOI: 10.3969/j.issn.1671-7171.2020.05.006 【Objective】 To explore the correlation between 24-hour ambulatory blood pressure and severity of coronary artery disease.【Methods】 From January 2018 to December 2018, 265 patients with coronary angiography in the Department of Geriatric Cardiology of Xiangya Hospital were selected as the research objects. All patients were monitored 24 hours of ambulatory blood pressure before coronary angiography (CAG) operation, and relevant parameters were recorded. The severity of coronary stenosis was expressed by Gensini score. According to the results of CAG, the patients were divided into coronary heart disease group (169 cases) and non coronary heart disease group (96 cases), and the differences of ambulatory blood pressure parameters between the two groups were compared; finally, according to the severity of coronary artery disease (Gensini score), patients in coronary heart disease group were divided into mild disease group, moderate disease group and severe disease group, and the differences of dynamic blood pressure parameters in different Gensini integral groups were compared.【Results】 There was no significant difference in age, weight, blood sugar, blood lipid, liver and kidney function between the two groups (P>0.05). Pearson correlation analysis showed that only the coefficient of variation of blood pressure was significantly correlated with Gensini score (r=0.179,P=0.029). In different Gensini integral groups, only the coefficient of variation of blood pressure had significant difference, and with the increase of Gensini integral, the higher the coefficient of variation of blood pressure was.【Conclusion】 Blood pressure variability is closely related to the severity of coronary lesions. In patients with coronary heart disease, the coefficient of blood pressure variability can be used as a predictor of the severity of coronary lesions. 2020 Vol. 37 (5): 658-660 [Abstract] ( 119 ) HTML (1 KB)  PDF  (0 KB)  ( 7 ) 661 Effect of Skull Repair on Cerebral Blood Flow and Neurocognitive Function in Patients with Flap Collapse Syndrome ZHOU Lu-qiao, XIE Liang, PENG Hu, et al DOI: 10.3969/j.issn.1671-7171.2020.05.007 【Objective】 To explore the effect of cranioplasty on cerebral blood flow, EEG and neurocognitive function in patients with skin flap collapse syndrome (SSFs).【Methods】 The clinical data of 56 patients with SSFs monitored by TCD were analyzed retrospectively. The changes of systolic peak velocity (PSV), diastolic peak velocity (EDV) and mean blood flow velocity (MV) of bilateral anterior cerebral artery (ACA), middle cerebral artery (MCA) and posterior cerebral artery (PCA) were monitored on the day before and one month after operation, and the abnormal rate of EEG and the improvement of neurocognitive function (FIM) before and after operation were compared.【Results】 The PSV、EDV、MV of ACA、MCA and PCA of affected side were lower than that of healthy side before operation, and the PSV、EDV、MV of ACA、MCA and PCA were higher than that before operation, the difference was statistically significant (P<0.05). SSFS preoperative FIM score (98.21±5.54) was lower than postoperative (112.36±3.86), and the difference was statistically significant (P<0.05). The total abnormal rate of postoperative EEG was 41.1%(23/56), which was lower than that of preoperative 46.4% (26/56), but the difference was not statistically significant (P=0.063>0.05).【Conclusion】 Skull repair can improve cerebral blood flow status and improve postoperative neurocognitive function in SSFS patients. 2020 Vol. 37 (5): 661-663 [Abstract] ( 148 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 664 Expression and Significance of Phosphatidylinositol Kinase-3 and Protein Kinase Akt in Mkeletal Muscle of Postmenopausal Hip Fracture Patients Mirarimu Multiza, LIN Hai-Shan, Eniwalj Da Maura, et al DOI: 10.3969/j.issn.1671-7171.2020.05.008 【Objective】 To investigate the expression levels and clinical significance of phosphatidylinositol kinase-3 (PI3K) and protein kinase (Akt) in skeletal muscle of postmenopausal hip fracture patients. 【Methods】 Prospectively selected a total of 80 post-menopausal patients who underwent surgery for hip fracture in our department from January 2017 to June 2019. Patients were divided into 4 different groups according to the dual-energy X-ray bone density (DXA) levels. They were normal bone mass group (-1≤T≤1, group A), bone mass reduction group (-2.5 <T <-1, group B), osteoporosis Group (T ≤-2.5, group C) and severe osteoporosis group(T ≤-2.5 with one or more brittle fractures, group D).A small amount of muscle tissue was taken from the lateral femoral muscle during the operation for RNA extract, quantitative Real-time PCR (RT-PCR) was detected for mRNA expression level of phosphatidyl inositol kinase- 3(PI3K)and protein kinase (Akt), while the protein expression levels of PI3K and Akt were measured by immunohistochemical technique. The correlation of PI3K and Akt with the bone density was analyzed as well. 【Results】 Both mRNA and protein expression levels of Akt and PI3K in the group D were higher than those in other groups (P<0.05).The mRNA and protein expression levels of Akt and PI3K in the group C was higher than that that in the group B (P<0.05). However, the mRNA and protein expression levels of Akt and PI3K were not statistically significant difference between the group A and B (P>0.05). The expression levels of AKT and PI3K were positively correlated with the bone mineral density of the patients. 【Conclusion】 The PI3K/Akt signaling pathway is activated in skeletal muscle of postmenopausal osteoporosis patients, and the mRNA and protein expression levels of PI3K and Akt are increased and also positively correlated with the degree of osteoporosis. It suggests that PI3K/Akt plays an important role in the regulation of bone-muscle formation and reconstruction. 2020 Vol. 37 (5): 664-667 [Abstract] ( 123 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 668 Effect of Endoscopic Retrograde Cholangiopancreatography on Serum Inflammatory Factors and Tumor Markers in Patients with Common Bile Duct Stones GUO Hui-li, ZHANG Yong-chao, JIN Da-qing, et al DOI: 10.3969/j.issn.1671-7171.2020.05.009 【Objective】 To investigate the effect of endoscopic retrograde cholangiopancreatography (ERCP) on serum inflammatory factors and tumor markers expression in patients with common bile duct stones. 【Methods】 A total of 200 patients with common bile duct stones treated in our hospital from January 2017 to December 2018 were divided into two groups, with 100 cases in each group. The observation group underwent endoscopic retrograde cholangiopancreatography, while the control group underwent routine laparotomy procedure. The indexes of surgery and levels of serum inflammatory factors (TNF-α,IL-1 and IL-6), CA19-9 and CEA expression were compared between the two groups. 【Results】 The operation time, intraoperative blood loss, anal exhaust time, abdominal pain relief time, and hospitalization time of the observation group were lower than those of the control group (P<0.05). There was no significant difference in the clearance rate between the two groups (P>0.05). The incidence of complication was 7.00% (7/100) in the observation group and 15.00% (15/100) in the control group. The difference was statistically significant (P<0.05). The serum levels of IL-1, IL-6, and TNF-α in the control group at d5 post-operation were higher than those before surgery and also higher than those in the observation group; the difference was statistically significant (P<0.05).The serum levels of CA19-9 and CEA in both groups at d3 and d5 after surgery were lower than that before surgery, while CA19-9 level in the observation group was lower than that in the control group. The difference was statistically significant (P<0.05). 【Conclusion】 Endoscopic retrograde cholangiopancreatography is effective in the treatment of common bile duct stones with less complications and faster recovery, and can effectively reduce the levels of CA19-9 and CEA. 2020 Vol. 37 (5): 668-670 [Abstract] ( 132 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 671 Effect of Dexmedetomidine and Sufentanil on Postoperative Patient-controlled Intravenous Analgesia in Patients with Colon Cancer YANG Xiao-yan, ZHU Guo-han, TAN Ai-ping DOI: 10.3969/j.issn.1671-7171.2020.05.010 【Objective】 To investigate the effect of dexmedetomidine combined with sufentanil on pain and comfort of Patient-controlled Intravenous Analgesia (PCIA) in patients with colon cancer after laparoscopic surgery. 【Methods】 A total of 104 patients undergoing laparoscopic colon cancer radical mastectomy were selected from our hospital. They were randomly divided into the observation group and the control group with 52 cases in each. Patients in the observation group recieved dexmedetomidine combined with sufentanil for PCIA analgesia, while patients in the control group only received single-agent sufentanil PCIA. Postoperative pain, comfort, serum Cor, β-EP, PGE2, IL-6 and adverse reactions were compared between the two groups. 【Results】 The VAS scores of the observation group patients at the corresponding time points from 2 hours to 48 hours after surgery were lower than those of the control group (P<0.05). The BCS scores of the observation group patients at the corresponding time points were higher than those of the control group (P<0.05); At time immediately after surgery, there was no significant difference in serum Cor, β-EP, PGE2, and IL-6 levels between the two groups (P>0.05), while 24 hours after surgery, the levels of serum Cor, β-EP, PGE2, and IL-6 in the observation group were lower than those in the control group (P<0.05). During the implementation of analgesia, the incidence of adverse reactions in the observation group was 9.62% which was lower than that in the control group 21.15% (P<0.05). 【Conclusion】 The application of PCIA with both dexmedetomidine and sufentanil in patients with colon cancer after laparoscopic surgery has a better effect on improving patients' pain and comfort experience. 2020 Vol. 37 (5): 671-673 [Abstract] ( 118 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 674 Clinical Effect of Vothixetine in the Treatment of First Major Depression YANG Dong, YU Yan, LIU Li-ni DOI: 10.3969/j.issn.1671-7171.2020.05.011 【Objective】 To analyze the safety and efficacy of vortioxetine in first-episode major depressive disorder patients. 【Method】 One hundred and twenty-three patients with major depression were randomly divided into two groups: the observation group (10 mg / D of vothixetine, n=61) and the control group (60 mg / D of duloxetine, n=62). The scores of HAMD-17 and SDS, HAMA and SAS were used as the main therapeutic indexes. Adverse reactions were recorded by side effect scale in safety assessment.【Results】 The effective rate of the observation group was 75.4%, the effective rate was 91.8%; the effective rate of the control group was 66.1%, the effective rate was 88.7%. There was no significant difference between the two groups in demonstrating efficiency and efficiency(P>0.05). After treatment, the HAMD scores of the two groups were lower than before treatment, the difference was statistically significant (P<0.05); the HAMD scores of the observation group were lower than that of the control group after 2 weeks of treatment, the difference was statistically significant (P<0.05). After 8 weeks of treatment, the scores of HAMA, HAMD, SDS and SAS in the two groups were significantly lower than those before treatment (P<0.05). There were 16 cases (26.2%) in the observation group and 18 cases (29.0%) in the control group. There was no significant difference between the two groups (P>0.05).【Conclusion】 The efficacy of vortioxetine in the treatment of depression is similar to that of duloxetine. Vortioxetine can relieve the depressive symptoms quickly and effectively, and can improve the anxiety symptoms of patients with depression, with good safety and tolerance. 2020 Vol. 37 (5): 674-676 [Abstract] ( 210 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 677 Effect of Flurbiprofen Axetil Combined with Sufentanil on Postoperative Analgesia of Cervical Cancer CHEN Jin-qiang, WANG Xian-chun, ZHANG De-xu, et al DOI: 10.3969/j.issn.1671-7171.2020.05.012 【Objective】 To observe the effect of flurbiprofen axetil combined with sufentanil on analgesia after laparotomy of cervical cancer. 【Methods】 One hundred patients with cervical cancer who underwent open surgery in our hospital were selected and randomly divided into the observation group and the control group, with 50 cases in each group. Patients in the observation group received sufentanil and flurbiprofen analgesia, while patients in the control group received sufentanil analgesia alone. The postoperative analgesic effect, serum β-EP (β-endorphin), Cor (cortisol), E(adrenaline), PGE2 (prostaglandin E2), substance P, and neuropeptide Y were compared between the two groups. The number of analgesia pump compressions and adverse reactions were recorded. 【Results】 The VAS scores of the observation group at 6 h, 12 h, 24 h, and 48h were lower than those of the control group (P< 0.05).; Compared to pre-surgery, there were not statistically significant differences in serum β-EP, Cor, E, PGE2, substance P, and neuropeptide Y levels at the time immediately after surgery (P>0.05). However, serum levels of β-EP, Cor, E, PGE2, substance P, and neuropeptide Y at 24 hours after surgery in the observation group were lower than those in the control group (P<0.05). The incidence of postoperative adverse reactions in the two groups was not statistically significant (P>0.05).Within 48 hours, the total number of analgesia pump compressions in the observation group was 5.4 ± 0.7 times, which was lower than that in the control group 7.2 ± 1.0 times; the difference between the two groups was statistically significant (P<0.05). 【Conclusion】 The application of flurbiprofen axetil combined with sufentanil for analgesia after laparotomy of cervical cancer can effectively improve the analgesic effect and reduce the levels of pain-related factors. 2020 Vol. 37 (5): 677-679 [Abstract] ( 129 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 680 Correlation Analysis of Serum SOD, NOX4 and ox-LDL Levels with Coronary Lesions in Patients with Coronary Heart Disease SUN Li, WANG Ying-zhong DOI: 10.3969/j.issn.1671-7171.2020.05.013 【Objective】 To investigate the level and significance of superoxide dismutase (SOD), nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) and oxidized low density lipoprotein (ox-LDL) in patients with coronary heart disease. 【Methods】 From May 2017 to may 2018, 84 patients with coronary heart disease were selected, including 39 patients with acute coronary syndrome (ACS group), 45 patients with stable angina pectoris (SAP group) and 50 healthy volunteers as the control group. The levels of ox-LDL, SOD and NOX4 were compared in each group. The coronary stenosis was scored by Gensini method. The correlation between the levels of ox-LDL, SOD and NOX4 and Gensini score was analyzed. 【Results】 The serum SOD level of the ACS group and the SAP group was significantly lower than that of the control group, and the serum NOX4 and ox LDL levels were significantly higher than that of the control group (P<0.05); the serum SOD level of the ACS group was significantly lower than that of the SAP group, and the serum NOX4 and ox-LDL levels were significantly higher than that of the SAP group (P<0.05). The Gensini score of the ACS group was (38.16±3.70), which was significantly higher than that of the SAP group (30.41±4.64). The difference was statistically significant (t=-8.374, P<0.05). There was a negative correlation between SOD and Gensini score (r= -0.354, P<0.05), and a positive correlation between NOx 4, ox-LDL and Gensini score (r=0.322, 0.315, P<0.05).【Conclusion】 The serum SOD level of patients with coronary heart disease decreased significantly, while the serum NOX4、ox-LDL level increased significantly, and the serum SOD、NOX4、ox-LDL level was closely related to the Gensini score of patients with coronary heart disease. 2020 Vol. 37 (5): 680-682 [Abstract] ( 137 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 683 Clinical Study of Adjusted doses of Apatinib Combined with Chemotherapy for Advanced Gastric Cancer Treatment XIAO Mi, WANG Mei-xing DOI: 10.3969/j.issn.1671-7171.2020.05.014 【Objective】 To study the clinical effect of different doses of Apatinib combined with chemotherapy for advanced gastric cancer treatment. 【Methods】 A total of 60 patients with advanced gastric cancer who failed second-line treatment were divided into the observation group (low-dose triple administration of apatinib combined with systemic chemotherapy, 30 cases) and the control group (continuous high dose of apatinib combined with systemic chemotherapy, 30 cases) by random number table method.The short-term effects of chemotherapy were compared between the two groups, and the adverse reactions were recorded during the treatment. The half-year survival rate in two groups were recorded by follow-up. 【Results】 The disease control rate of the observation group after 2 courses of treatment was higher than that of the control group; the difference was statistically significant (P<0.05).There were no significant difference in the severity of adverse reactions such as hypertension, proteinuria, hemorrhage, anemia and leukopenia between the two groups (P>0.05).The severity of gastrointestinal and hand-foot skin reactions in the observation group was significantly less than that in the control group (P<0.05).The half-year survival rate of the observation group was 43.33% (13/30), which was not significantly different from the control group 30.00% (9/30) (P>0.05). 【Conclusion】 Low-dose triple administration of apatinib combined with systemic chemotherapy in the treatment of advanced gastric cancer improves the efficacy of chemotherapy, reduces adverse reactions and has high clinical application value. 2020 Vol. 37 (5): 683-685 [Abstract] ( 154 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 686 Clinical Efficacy of Donepezil Hydrochloride Combined with Nimodipine in the Treatment of Elderly Patients with Alzheimer's Disease GONG Ping, ZHANG Wei, SUN Yi-nan, et al DOI: 10.3969/j.issn.1671-7171.2020.05.015 【Objective】 To investigate the effect of donepezil hydrochloride combined with nicergoline on the efficacy and stress status of elderly patients with Alzheimer's disease. 【Methods】 A retrospective analysis was made of 115 elderly patients with AD who were treated in our hospital from June 2015 to January 2018. According to the treatment methods, they were divided into observation group (n=65) and control group (n=50). The control group was treated with nimodipine, and the observation group was treated with donepezil on the basis of the control group. After treatment for one month, the therapeutic effect and the levels of serum homocysteine (Hcy), neuron specific enolase (NSE), central neuron specific protein (S100 β), orgotein (SOD), glutathione peroxidase (GSH Px), malondialdehyde (MDA), the end product of lipoxygenation, and the incidence of adverse reactions were compared. 【Results】 The total effective rate of the observation group was 92.31%, which was significantly higher than that of the control group (78.00%) (P<0.05). Before treatment, there was no significant difference in serum Hcy, NSE and S100βlevels between the two groups (P>0.05); after treatment, the serum Hcy, NSE, S100 β and MDA levels of the two groups decreased (P<0.05), SOD and GSH Px levels increased, SOD and GSH PX levels of the observation group were significantly higher than those of the control group (P< 0.05), and the changes of the observation group were more obvious. The total incidence of adverse reactions in the observation group was 6.15%, which was significantly lower than that in the control group (22.00%) (P<0.05).【Conclusion】 Donepezil hydrochloride combined with nimodipine in the treatment of elderly patients with AD has a significant effect, can significantly reduce the level of Hcy, NSE, S100β, regulate the level of SOD, GSH Px and MDA, and has a high safety, which is worthy of clinical application. 2020 Vol. 37 (5): 686-688 [Abstract] ( 127 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 689 Effects of Single-Direction Thoracoscopic Lobectomy on Oxygen Free Radicals and Vascular Endothelial Growth Factor in Lung Cancer Patients DENG Yong, SHI Jin, YANG Wen-gang, et al DOI: 10.3969/j.issn.1671-7171.2020.05.016 【Objective】 To explore the effects of single-direction thoracoscopic lobectomy on oxygen radicals and vascular endothelial growth factor in patients with lung cancer. 【Methods】 The clinical data of 136 patients with lung cancer treated in our hospital were retrospectively analyzed and divided into thoracoscopic group (underwent single-direction thoracoscopic lobectomy) and control group (underwent radical thoracotomy) according to the treatment methods. Operation time, blood loss, thoracic drainage and hospitalization time were compared between the two groups. Serum levels of SOD, MDA, GSH-px, VEGF, MMP-9 and CRP were compared between the two groups before and 24h after surgery. Postoperative complications were compared between the two groups. 【Results】 The operation time, bleeding volume, pleural drainage and hospitalization time of patients in the thoracoscopic group were lower than those in the control group (P<0.05); the serum SOD and GSH-px levels of patients in the thoracoscopic group were significantly higher than those in the control group (P<0.05), and the MDA,VEGF,MMP-9 and CRP levels were lower than those in the control group (P<0.05).【Conclusion】 Single-direction thoracoscopic lobectomy is effective in the treatment of lung cancer. the trauma to patients is small, which can reduce the oxidative stress response of patients and reduce the expression of VEGF, and the incidence of complications is low, which is worthy of clinical application. 2020 Vol. 37 (5): 689-692 [Abstract] ( 107 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 693 Significance of AR mRNA and ERα mRNA Levels in PBMCs of Patients with Chronic Hepatitis B SUN Jian, WANG Su DOI: 10.3969/j.issn.1671-7171.2020.05.017 【Objective】 To investigate mRNA expression and significance of androgen receptor (AR) and estrogen receptor α (ERα) in peripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis B. 【Methods】 A total of 145 patients with chronic hepatitis B treated in our hospital from January 2018 to January 2019 (the observation group) and 145 healthy volunteers were selected as the control group. Levels of mRNA expression of AR and ERα in PBMCs was detected by real-time fluorescence quantitative polymerase chain reaction. 【Results】 The relative expression level of AR mRNA in PBMCs of the observation group was (0.351±0.101), was significantly lower than that of the control group (P<0.05), while the relative expression level of ERα mRNA was (0.643±0.121), was significantly higher than that of the control group (P<0.05). There were no significant differences in AR and ERα mRNA expression in PBMCs of patients with different gender, age, HBeAg expression and HBV viral load in the observation group (P>0.05).The relative expression level of AR mRNA in PBMCs of patients with liver fibrosis stage S3-S4 in observation group was (0.284±0.097), which were significantly lower than that in patients with S 0 and S1-S3 stage (P<0.05), while the relative expression level of ERα mRNA was (0.738±0.110), which were significantly higher than that in patients with S0 and S1-S3 stage (P<0.05). 【Conclusion】 In PBMCs of chronic hepatitis B patients, the AR mRNA expression is down-regulated and the ERα mRNA expression is up-regulated, which are related to the stage of hepatic fibrosis. 2020 Vol. 37 (5): 693-695 [Abstract] ( 102 ) HTML (1 KB)  PDF  (0 KB)  ( 7 ) 696 Relationship between CD64 and Wagner Grade of Diabetic Foot Ulcer SUN Hao-jie, GENG Hou-fa, LIANG Jun DOI: 10.3969/j.issn.1671-7171.2020.05.018 【Objective】 To explore the relationship between peripheral blood CD64 level and Wagner grading level of diabetic foot. 【Methods】 From November 2017 to December 2018, 162 patients with diabetic foot were divided into two groups according to the Wagner classification (severity): Wagner 2-3 (93 cases, group A), Wagner 4-5 (69 cases, group B). The levels of peripheral blood CD64, serum procalcitonin (PCT), C-reactive protein (CRP) and leukocyte (WBC) were compared between the two groups, The correlation between peripheral blood CD64 and other indexes was analyzed, and the relationship between CD64 and Wagner grade of diabetic foot was analyzed by logistic regression. The ROC curve was drawn, the area under the curve was calculated, and the sensitivity and specificity of CD64 in predicting the severity of diabetic foot were analyzed.【Results】 The levels of CD64, CRP, PCT and WBC in the group A were significantly higher than those in the group B (P<0.05). The correlation analysis showed that CD64 was positively correlated with CRP, PCT and WBC (r=0.86, 0.84, 0.62, respectively; P<0.05). Multivariate logistic regression analysis: the course of diabetic foot, CD64 and smoking were independent risk factors for the severity of diabetic foot disease (P<0.05). The area under ROC curve (0.892) showed that CD64 was related to the severity of diabetic foot. According to the maximum Youden index, when the critical value of CD64 was 3.38, its sensitivity was 81.2%, and its specificity was 91.4%.【Conclusion】 CD64 is related to Wagner's grade of diabetic foot, and high expression level of CD64 indicates that the condition of diabetic foot is more serious. 2020 Vol. 37 (5): 696-698 [Abstract] ( 105 ) HTML (1 KB)  PDF  (0 KB)  ( 8 ) 699 Value of Amplitude Integrated Electroencephalogram in Evaluating the Prognosis of Neonatal Asphyxia in Tibet WU Tian-qi, WU Hong, WANG Xiu, et al DOI: 10.3969/j.issn.1671-7171.2020.05.019 【Objective】 To explore the prognostic value of amplitude integrated electroencephalogram (aEEG) in neonatal asphyxia in Tibet.【Methods】 From September 2018 to October 2019, 75 Tibetan term asphyxiated newborns admitted to NICU were selected as the observation group, and 40 non asphyxiated Tibetan term newborns were selected as the control group. It was recorded by Nicolet monitor. The continuous EEG, SWC maturity and aEEG abnormality were compared between the two groups. The growth and development of the children with abnormal aEEG and normal aEEG were observed after 6 months follow-up.【Results】 There were 37 cases (49.33%) of continuous EEG in the observation group and 39 cases (97.50%) in the control group. The proportion of continuous EEG in the observation group was lower than that in the control group, the difference was statistically significant (χ2= 27.004, P<0.05).The proportion of SWC maturity in the observation group was 60.00% (45/75), which was lower than 95.00% (38/40) in the control group. The difference was statistically significant (χ2= 15.912, P<0.05). The abnormal rate of aEEG in the observation group was 57.33% (43/75), which was higher than 12.50% (5/40) in the control group. The difference was statistically significant (χ2= 21.563, P<0.05). There was a significant difference in the rate of abnormal growth and development between aEEG and normal children (χ2= 4.756, P<0.05). 【Conclusion】 aEEG has an important evaluation value for the prognosis of neonatal asphyxia in Tibet. It can be used to evaluate the brain injury of the children in the early stage. According to the evaluation results, the clinicians can give active and effective treatment, which is of great significance for improving the prognosis of the children. 2020 Vol. 37 (5): 699-701 [Abstract] ( 103 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 702 Analgesic Effect and Stress Response of Multimodal Analgesia in Laparoscopic Hysteromyomectomy LUO Hai-ming, JING Shan-liang, KUANG Yan DOI: 10.3969/j.issn.1671-7171.2020.05.020 【Objective】 To explore the analgesic effect and stress response of multimodal analgesia in the application of laparoscopic hysteromyomectomy.【Methods】 A total of 100 patients with uterine leiomyoma admitted to our hospital were divided into the observation group and the control group by random table method, with 50 cases in each group. Laparoscopic hysteromyomectomy was performed in both groups. The control group received routine general anesthesia plus post-operative PCIA, while the observation group received multimodal analgesia. The pain score of visual analogue scale (VAS), the amount of analgesics used at different time and the pressing times of analgesic pumps were recorded and compared between the two groups. Postoperative stress response indicators such as angiotensin Ⅱ(AngⅡ), cortisol (Cor), corticotropin releasing hormone (CRH), norepinephrine (NE) levels were measured in both groups.The adverse reactions were also compared in the two groups.【Results】 The pain scores of the observation group at 2, 4, 12, 24 and 48 hours after treatment were lower than those of the control group (P<0.05).The dosage of analgesics and the number of pressing analgesic pumps in the observation group were lower than those in the control group at 12h, 24h and 36h after treatment (P<0.05). AngⅡ, Cor, CRH and NE levels in the observation group were significantly lower than those in the control group (P<0.05).The total incidence of adverse reactions in the two groups was not significant difference (P>0.05).【Conclusion】 The analgesic effect of multimodal analgesia in laparoscopic hysteromyomectomy is remarkable. It can reduce stress response which has high clinical application value. 2020 Vol. 37 (5): 702-704 [Abstract] ( 109 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 705 Effect of Self-made Yiai decoction Combined with XELOX on Postoperative Outcomes of Gastric Cancer YIN Ting-li DOI: 10.3969/j.issn.1671-7171.2020.05.021 【Objective】 To investigate the effects of self-made Yiai decoction combined with capecitabine and oxaliplatin (XELOX regimen) on tumor markers, susceptibility genes and quality of life of patients with gastric cancer after operation. 【Methods】 A total of 86 cases of patients with gastric cancer treated who underwent radical gastrectomy were randomly divided into two groups, with 43 cases in each group. Patients in the control group received XELOX chemotherapy after operation, while patients in the combined group received self-made Yiai decoction combined with XELOX regimen. The curative effects were compared between the two groups. 【Results】 After chemotherapy the levels of carcinoembryonic antigen, carbohydrate antigen 125, carbohydrate antigen 199, gastrin and vasoactive intestinal peptide in the two groups were significantly decreased (P<0.05), while mRNA levels of breast cancer susceptibility gene 1 and breast cancer susceptibility gene 2 were significantly increased (P<0.05). The physical, functional, social family, emotional, doctor-patient relationship and cancer additional attention scores were significantly improved (P<0.05). Those changes in the combined group were more remarkable than in the control group (P<0.05). The degree of bone marrow suppression in the combined group was significantly lower than that in the control group (P<0.05). 【Conclusion】 Self-made Yiai decoction can significantly improve the curative effect of XELOX chemotherapy after radical gastrectomy, reduce the levels of serum tumor markers, and improve the quality of life of patients. 2020 Vol. 37 (5): 705-707 [Abstract] ( 137 ) HTML (1 KB)  PDF  (0 KB)  ( 9 ) 708 Effect of Sufentanil Target-Controlled Infusion on Postoperative Analgesia, Stress Response and Immune Function after Laparoscopic Surgery LIAO Hai-ming, YU Fan, DENG Qing-shan, et al DOI: 10.3969/j.issn.1671-7171.2020.05.022 【Objective】 To investigate the effect of sufentanil target-controlled infusion on postoperative analgesia, stress response and immune function after laparoscopic surgery. 【Methods】 A total of 82 patients with laparoscopic surgeries in our hospital were collected in the study. The randomized digital table was used to divide these cases into the control group (n=42) and the observation group (n=40). Patients in the control group received intravenous analgesia after operation, while patients in the observation group received the target-controlled analgesic of citrate sufentanil after operation. The postoperative sedation and analgesia scores of the two groups were compared, meanwhile, the concentrations of substance P and β endorphin and levels of CD4+, CD8+, CD4+/CD8+ were measured before and after treatment.【Results】 The analgesia and sedation scores between the two groups had significant difference (P<0.05). After treatment, the level of substance P in the observation group was significantly lower, while the level of β-endorphin in the observation group was higher than that in the control group and before treatment (P<0.05). The level of CD4+ in observation group after treatment was lower than that before treatment, and the CD4+ level in the observation group was also lower than that in the control group (P<0.05). The levels of CD8+ and CD4+/CD8+ had no significant difference between the two groups (P>0.05). 【Conclusion】 The application of the sufentanil target-controlled infusion shows good postoperative analgesia effect, which reduces the postoperative stress response and immune function regulation for patients after laparoscopic surgery. It is worthy of further clinical promotion. 2020 Vol. 37 (5): 708-711 [Abstract] ( 103 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 712 Effect of Repeated Uterine Prolapse on Progression of Cervical and Vaginal lesions GU Wen-wen, DING Yi, CHEN Ji-ming, et al DOI: 10.3969/j.issn.1671-7171.2020.05.023 【Objective】 To investigate the effect of repeated uterine prolapse on the progression of cervical and vaginal lesions. 【Methods】 A total of 100 patients with repeated uterine prolapse admitted to our hospital from January 30, 2015 to June 30, 2016 were selected as the observation group. At the same period,100 healthy women who received physical examination in our hospital were selected as the control group. The length of the cervix (anterior-posterior diameter and transverse) and the length of the cervicovaginal were measured. The cervical size and the length of the cervix were compared between the two groups. The subjects were followed up for 3 years. The incidence of cervical lesions and vaginitis were recorded. The relationship between cervical lesions and cervix length was analyzed. 【Results】 The incidence of cervical enlargement and cervical vaginal prolongation was higher in the observation group than in the control group (P<0.05); and the proportion of new cervical enlargement and cervical vaginal extension in the observation group was higher during the follow-up period than that in the control group (P<0.05). After 3 years of follow-up, the incidence of cervical lesions and vaginitis in the observation group were higher than those in the control group (P<0.05).The incidence of cervical lesions and vaginitis in the observation group was correlated with uterine prolapse grade (P<0.05). The incidence of cervical uterine prolapse and vaginitis in patients with grade Ⅳ was higher than that in patients with grade Ⅰ, Ⅱ and Ⅲ (P<0.05). The incidence of cervical lesions in patients with cervical enlargement before the follow-up was higher than that in patients with non-cervical enlargement and the incidence of vaginitis in patients with cervical vaginal extension before the follow-up was higher than that in patients with non-cervical vaginal extension (P<0.05). 【Conclusion】 Repeated uterine prolapse can increase the incidence of cervical and vaginal lesions, and the occurrence of cervical and vaginal lesions is closely related to the size of the cervix, the length of the cervicovaginal and the uterus prolapse. 2020 Vol. 37 (5): 712-714 [Abstract] ( 113 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 715 Effect of Proximal Subtotal Gastrectomy and Jejunal Interposition Surgery on Gastrointestinal Function, Nutritional Status and Short-term Survival after proximal gastrectomy LUO Liang, WU Chen, CHEN Jin, et al DOI: 10.3969/j.issn.1671-7171.2020.05.024 【Objective】 To investigate the effect of proximal subtotal gastrectomy and jejunal interposition surgery on gastrointestinal function, nutritional status and short-term survival after proximal gastrectomy. 【Methods】 The clinical data of 105 patients with proximal gastric cancer treated surgically in our hospital from August 2013 to May 2017 were retrospectively analyzed. According to the different treatment methods, patients were divided into the control group (n=43) and the observation group (n=62). The patients in the control group were treated with radical total gastrectomy plus esophagojejunal Roux-en-Y anastomosis. While patients in the observation group were treated with proximal subtotal gastrectomy and jejunal interposition surgery. The perioperative indicators and the incidence of complications were compared between the two groups. The gastrointestinal function was compared between the two groups as well, including the levels of gastrointestinal hormones(GAS and MLT), and the time of exhaust after operation. Nutritional status, 1-year and 2-year survival rates were recorded between the two groups. 【Results】 The operation time of the observation group was significantly longer than that of the control group (P<0.05), while the amount of bleeding and lymphatic clearance were significantly lower than those of the control group (P<0.05).There was no statistical difference in the length of incision between the two groups (P>0.05). Three months after operation, the levels of GAS and MTL in both groups were lower than those before operation, however, the level of GAS in the observation group was higher than that in the control group (P<0.05). There were no significant differences in the levels of MLT and the time of exhaust after operation between the two groups (P>0.05). The incidence of dumping syndrome, Roux detention syndrome and reflux esophagitis in the observation group was significantly lower than that in the control group (P<0.05). There was no significant difference in incision infection, pulmonary infection, abdominal infection, anastomotic leakage and intestinal obstruction between the two groups (P>0.05). The levels of hemoglobin, albumin and prognostic nutrition index (PNI) in the observation group were significantly higher than those in the control group (P<0.05). There was no statistically significant difference in 1-year and 2-year survival rates between the two groups (P>0.05). 【Conclusion】 Proximal subtotal gastrectomy and jejunal interposition surgery is more effective in the treatment of proximal gastric cancer. It can reduce the incidence of dumping syndrome, Roux retention syndrome and reflux esophagitis, which can improve nutritional status. It is an ideal method for digestive tract reconstruction after proximal gastric cancer surgery. 2020 Vol. 37 (5): 715-717 [Abstract] ( 107 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 718 Metoprolol Combined with Eplidone in the Treatment of 52 Cases of Hypertension with Heart Failure ZHOU Lin DOI: 10.3969/j.issn.1671-7171.2020.05.025 【Objective】 To investigate the clinical efficacy and safety of metoprolol combined with eplidone in the treatment of hypertensive heart failure. 【Methods】 A total of 104 patients with hypertensive heart failure who were treated in our hospital were enrolled in the study. The patients were divided into two groups,control group (n=52) and observation group (n=52). View Blood pressure, amino terminal brain natriuretic peptide precursor (NT-proBNP), 6min walking test distance (6MWT), cardiac function index, clinical efficacy and adverse reaction rate were observed before and after treatment in two groups. 【Results】 The total effective rate of the observation group was 92.31% (48/52), significantly higher than that of the control group 67.31% (35/52), the difference was statistically significant (P<0.05). After treatment, SBP, DBP and NT-proBNP in plasma of the two groups were lower than before treatment, and 6MWT was higher than before treatment. SBP, DBP and NT-proBNP, 6MWT in plasma of the observation group were significantly improved compared with the control group (P<0.05). After treatment, LVEDd and LVESD of the two groups were lower than before treatment, LVEF was higher than before treatment, NYHA classification was better than before treatment, and the heart function index and NYHA classification of the observation group were better than that of the control group, the difference was statistically significant (P>0.05). During the treatment of the two groups, the incidence of adverse reactions such as nausea and vomiting, slow heart rate, dizziness, rash, hyperkalemia, joint pain were compared, and the difference was not statistically significant (11.54% vs 9.62%,P>0.05).【Conclusion】 Metoprolol combined with eplidone has a good clinical effect in the treatment of hypertension combined with heart failure. It can significantly reduce the blood pressure of patients, improve the heart function of patients, and has a high safety. It is worth clinical application. 2020 Vol. 37 (5): 718-720 [Abstract] ( 117 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 721 Comparison of Analgesic Effects between Oxycodone and Sufentanil after Cesarean Section HE Lei, ZHANG Jing-jing, WANG Shao-lin DOI: 10.3969/j.issn.1671-7171.2020.05.026 【Objective】 To explore the effects of oxycodone and sufentanil on analgesia after cesarean section. 【Methods】 A total of 160 pregnant women who underwent cesarean delivery in our Department of Obstetrics were randomly divided into two groups (the oxycodone group and the sufentanil group),with 80 cases in each. Patients in the two groups received intravenous different drug-controlled analgesia after operation. Pain degree scores (VAS) at different time points and under uterine contraction after surgery, postoperative pump compression intravenous analgesia (PCIA), serum prostaglandin E2 (PGE2), serotonin (5-HT) and the occurrence of adverse reactions were recorded and compared between the two groups. 【Results】 There were no significant differences in VAS scores between the oxycodone group and the sufentanil group at 2 h, 6 h, 12 h, 24 h, and 48 h after surgery (P>0.05). Total compression times of the intravenous analgesia pump at 24 h, 48 h after surgery in the oxycodone group were not significant differences from those in the sufentanil groups (P>0.05). Before surgery, there were no statistical differences in levels of serum PGE2 and 5-HT between the two groups (P>0.05); However, the serum PGE2 and 5-HT levels of the two groups 24 hours after delivery were higher than their corresponding preoperative group (P<0.05). The incidence of maternal adverse reactions in the oxycodone group was 7.50%. It was lower than that of the sufentanil group 18.75% (P<0.05). 【Conclusion】 The effect of oxycodone and sufentanil for analgesia after cesarean section is not much different, but the former has a lower adverse reaction rate. 2020 Vol. 37 (5): 721-723 [Abstract] ( 121 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 724 Level Changes and Clinical Significance of Procalcitonin and N-terminal pro-B-type Natriuretic Peptide in Patients with Acute Exacerbation of Chronic Pulmonary Heart Disease CAI Qiu-yan, CHEN Jing DOI: 10.3969/j.issn.1671-7171.2020.05.027 【Objective】 To explore clinical relationship of procalcitonin (PCT) and N-terminal pro-B-type natriureticpeptide (NT-proBNP) with the severity of acute exacerbation of chronic pulmonary heart disease (AECPHD). 【Methods】 Patients with chronic pulmonary heart disease (n=35, the CPHD group) and with AECPHD (n=32), the AECPDH group) were selected for the study. All were treated routinely according to clinical guidelines. Sputum specimens were collected for bacterial quantitative culture before treatment. Levels of PCT and NT-proBNP in patients with AECPHD and CPHD were measured before and after treatment. 【Results】 After treatment, the plasma PCT and NT-proBNP levels of the two groups were lower than before treatment, while PCT and NT-proBNP levels in patients with AECPHD were significantly higher than those in patients with CPHD. The difference was statistically significant (P<0.05). The positive rate of sputum culture (PPM ≥ 107 CFU/mL) in patients with AECPHD was 28.13% (9/32), which was higher than that in patients with CPHD 8.57% (3/32, P<0.05). 【Conclusion】 PCT and NT-proBNP levels can be used as potential indicators for the severity and treatment in patients with AECPHD. 2020 Vol. 37 (5): 724-726 [Abstract] ( 118 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 727 Effect of GnRH- α Combined with Reverse Addition Therapy after Operation of Endometriosis HUO An-ni, WANG Chun-tong, LIU Yue-he DOI: 10.3969/j.issn.1671-7171.2020.05.028 【Objective】 To study the effect of gonadotropin releasing hormone agonist (GnRH-α) combined with reverse addition therapy on the maintenance of estrogen level after surgery in patients with Endometriosis (EMS). 【Methods】 A total of 104 patients undergoing laparoscopic surgery in our hospital from December 2016 to December 2018 were randomly divided into two groups. Patients in the control group received GnRH-α after operation, while patients in the observation group were treated with GnRH- α combined with reverse addition therapy after surgery. The clinical efficacy of the two groups after treatment was compared and analyzed. 【Results】 The total effective rate was 96.15% (50/52) in the observation group and was 78.85% (41/52) in the control group. The total effective rate in the observation group was higher than that in the control group (P<0.05). Before treatment, there were no significant differences in the levels of estradiol (E2), progesterone (P), testosterone (T), soluble intercellular adhesion factor-1 (sICAM-1), matrix metalloprotease-9 (MMP-9) and IL-8 (IL-8) between the two groups (P>0.05). After treatment, E2, P, T, sICAM-1, MMP-9 and IL-8 in the observation group were significantly lower than those in the control group (P<0.05). There was no statistically significant difference in the adverse reaction rate between the observation group (11.54%) and the control group (13.46%) (P>0.05). 【Conclusion】 GnRH- α combined with reverse addition therapy can improve the hormone level of patients, reduce inflammatory reaction, improve the therapeutic effect and do not increase the adverse reactions after surgery in patients with Endometriosis. 2020 Vol. 37 (5): 727-729 [Abstract] ( 111 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 730 Changes and Clinical Significance of Serum mir-198 and mir-744 Expression in Patients with Gastric Cancer ZHANG Hua DOI: 10.3969/j.issn.1671-7171.2020.05.029 【Objective】 To investigate the changes and clinical significance of serum mir-198 and mir-744 expression in patients with gastric cancer. 【Methods】 From January 2016 to December 2018, 106 gastric cancer patients admitted to our hospital were selected as the study object (observation group), and 35 subjects with normal physical examination in our hospital during the same period were selected as the control group. The qRT PCR was used to detect the expression of mir-198 and mir-744. The expression levels of mir-198 and mir-744 in serum of the two groups were compared, and the relationship between them and the clinicopathology of gastric cancer patients was analyzed. The diagnostic efficacy of the expression levels of mir-198 and mir-744 in serum for gastric cancer was analyzed by ROC curve.【Results】 The serum mir-198,mir-744 expression level of the observation group was significantly higher than that of the control group, and the serum mir-198,mir-744 expression levels of patients with stage Ⅲ/Ⅳ, invasion of serosa and high/median differentiation of the serum mir-198,mir-744 expression levels were significantly higher in the observation group than those in stage Ⅰ/Ⅱ, non-invasive serosa and low or undifferentiated patients, respectively, and the differences were statistically significant (P<0.05). ROC curve analysis showed that the lower AUC area of serum mir-198 for the diagnosis of gastric cancer was 0.863, the critical value was 3.93, the sensitivity was 76.42% and the specificity was 88.57%. the lower AUC area of serum mir-744 for the diagnosis of gastric cancer was 0.806, the critical value was 2.48, the sensitivity was 78.30% and the specificity was 77.14%. There was no significant difference in diagnostic efficacy (Z=1.056, P=0.291).【Conclusion】 Mir-198 and mir-744 are highly expressed in the serum of patients with gastric cancer, which are related to the clinical stage, infiltration depth and differentiation degree of patients. They can be used as potential molecular markers for diagnosis and treatment of gastric cancer. 2020 Vol. 37 (5): 730-732 [Abstract] ( 108 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 733 Relationship of Platelet-to-Lymphocyte Ratio and Lipoprotein-associated phospholipase A2 with Post Stroke Depression ZHANG Bei, TU Qiang DOI: 10.3969/j.issn.1671-7171.2020.05.030 【Objective】 To investigate the correlation of platelet-to-lymphocyte ratio(PLR) and lipoprotein-associated phospholipase A2(Lp-PLA2) with post stroke depression (PSD) in patients with acute ischemic stroke (AIS). 【Methods】 According to the Hamilton Depression Scale 17 Versions (HAMD-17) score,100 patients with AIS were assigned to the PSD group (32 cases with scores ≥8) and the non-PSD group (68 cases with scores ≤7).Statistical methods were used to compare the general clinical data, laboratory results and the score of the National Institutes of Health Stroke Scale(NIHSS) between the two groups. Pearson correlation analysis was made between the statistically significant indexes and HAMD-17 score, and logistic regression analysis was used to predict the influencing factors of PSD. 【Results】 The incidence rate of PSD was 32% after AIS follow-up for three months. PLR, Lp-PLA2 level and NIHSS score in the PSD group were higher than those in the non-PSD group, with statistically significant difference (P<0.05). Pearson correlation analysis showed that PLR, Lp-PLA2 and NIHSS score were positively with the HAMD-17 score (P<0.05). Logistic regression analysis showed that PLR, Lp-PLA2 and NIHSS scores were independent predictors of PSD occurrence. 【Conclusion】 PLR, Lp-PLA2 and NIHSS score were significantly higher in PSD patients. The severity of PSD was positively correlated with PLR, Lp-PLA2 and NIHSS score. PLR, Lp-PLA2 and NIHSS score were independent predictors of PSD. 2020 Vol. 37 (5): 733-736 [Abstract] ( 112 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 737 Application of Dexmedetomidine Combined with Remifentanil in Anesthesia of Gynecological Daytime Operation GAO Jian-xin, YU Ke, YANG Wei-tao DOI: 10.3969/j.issn.1671-7171.2020.05.031 【Objective】 To observe the anesthesia effect of dexmedetomidine combined with remifentanil in gynecological daytime operation.【Methods】 One hundred cases of gynecological day surgery were selected,and were divided into dexmedetomidine combined with remifentanil group (observation group) and propofol group (control group) according to the random number table method. Both groups were treated with plasma concentration target controlled infusion of intravenous anesthesia (TCI), the observation group with dual-channel target controlled infusion of dexmedetomidine (plasma target concentration 0.3 ng/mL) and remifentanil (plasma target concentration 1.5 ng/mL), and the control group with target controlled infusion of propofol (plasma target concentration 3.0μg/mL). When the patient showed body movement during operation, the observation group was injected with remifentanil and the control group with propofol until body movement disappeared.The mean arterial pressure (map), blood oxygen saturation (SpO2), heart rate (HR) of patients before anesthesia induction (T1), before operation (T2), during operation (T3) and during recovery (T4) were recorded; the physical activity of patients during operation was recorded; the respiratory inhibition (SpO2 < 90% or apnea > 15s) was recorded ;and the recovery time (from the end of operation to wake up and open eyes, handshake according to instructions) and the occurrence of postoperative uterine contraction pain (VAS score) were recorded, and the time from drug withdrawal to anesthesia induction in the two groups were recorded.【Results】 There was no significant difference in map, SpO2 and HR between the two groups at T1 and T3 (P>0.05); map, SpO2 and HR in the observation group at T2 were significantly higher than those in the control group (P<0.05); The MAP and HR in the control group at T4 were significantly higher than those in the observation group (P<0.05), but there was no significant difference in SpO2 between the two groups (P>0.05). The number of body movement of patients in the observation group was significantly less than that in the control group (P<0.05), and the recovery time was significantly shorter than that in the control group (P<0.05), but there was no significant difference in the incidence of respiratory inhibition between the two groups (P>0.05). The time of anesthesia induction in the observation group was longer than that in the control group (P<0.05). 【Conclusion】 In the gynecological daytime operation, dexmedetomidine combined with remifentanil has a definite anesthesia effect, can obviously reduce the postoperative uterine contraction pain, shorten the recovery time and quality of patients, and improve the comfort of patients and operation.However, it is more expensive and longer than propofol.For the patients with propofol contraindications in gynecological daytime surgery, the combination of dextromethoridine and remifentanil anesthesia is an appropriate choice. 2020 Vol. 37 (5): 737-739 [Abstract] ( 128 ) HTML (1 KB)  PDF  (0 KB)  ( 15 ) 740 Effect of Ultrasound-guided Horizontal Abdominal Muscle Block on Preventive Analgesia and Inflammatory Factors in Patients Undergoing Gynecological Laparoscopic Surgery ZHAO Wang-cheng, YAN Jun DOI: 10.3969/j.issn.1671-7171.2020.05.032 【Objective】 To observe the effect of ultrasound-guided transverse abdominal muscle block on preventive analgesia and inflammatory factors in patients undergoing gynecological laparoscopic surgery. 【Methods】 From February 2016 to January 2018, 120 patients undergoing gynecological laparoscopic surgery were randomly divided into observation group (n=60) and control group (n=60).The observation group underwent ultrasound-guided abdominal transverse muscle plane block anesthesia before anesthesia, while the control group underwent ultrasound-guided abdominal transverse muscle plane block anesthesia after operation. The pain status (pain VAS score),analgesic recovery rate, comfort scale (BCS) score and inflammatory factors (tumor necrosis factor-α, IL-6, IL-2, IL-8 and postoperative infection) levels of the two groups at different time points were compared.【Results】 The rest and exercise VAS scores of the patients in the observation group were significantly lower than 1 h after operation (P<0.05); the VAS scores of the patients in the control group at different time points after operation were significantly lower than those in the control group (P<0.01). The postoperative remediation rate (1.67%) in the observation group was significantly lower than that in the control group (11.67%)(P<0.05); the BCS score in the observation group was significantly lower than that in the control group at different time points after operation (P<0.05); and the TNF-α、IL-6、IL-2、IL-8 level in the observation group was significantly lower than that in the control group at 24 h after operation (P<0.05). 【Conclusion】 The use of ultrasound-guided horizontal abdominal muscle block before anesthesia in gynecological laparoscopic surgery can effectively play a preventive analgesic role, reduce the level of postoperative inflammatory factors, and promote the postoperative rehabilitation of gynecological laparoscopic surgery patients. 2020 Vol. 37 (5): 740-742 [Abstract] ( 104 ) HTML (1 KB)  PDF  (0 KB)  ( 8 ) 743 Changes and Significance of Serum ACT-A, Cys-C and BNP Levels in Patients with Acute Heart Failure SUN Jing, YANG Kun, HE Yong DOI: 10.3969/j.issn.1671-7171.2020.05.033 【Objective】 To investigate the levels and significance of serum activin A (ACT-A), cystatin C (Cys-C) and B-type brain natriuretic peptide (BNP) in patients with acute heart failure. 【Methods】 From January 2017 to March 2018, 98 AHF patients (observation group) and 100 healthy persons in the same period were selected as the control group. The levels of ACT-A, BNP and Cys-C were compared between the two groups, and the levels of ACT-A, BNP and Cys-C in AHF patients with different NYHA grades were compared.【Results】 The serum levels of act-a, Cys-C and BNP in the observation group were significantly higher than those in the control group (P<0.05). The serum levels of ACT-A, Cys-C and BNP in patients with NYHA grade Ⅲ~Ⅳ in the observation group were significantly higher than those in patients with gradeⅠ~Ⅱ (P<0.05). Pearson correlation analysis showed that ACT-A was positively correlated with BNP (r=0.672, P<0.05), but not with Cys-C (P>0.05); Cys-C was not correlated with BNP (P>0.05).【Conclusion】 The serum levels of ACT-A, Cys-C and BNP are significantly increased in AHF patients, which is related to the severity of the disease. 2020 Vol. 37 (5): 743-745 [Abstract] ( 491 ) HTML (1 KB)  PDF  (0 KB)  ( 8 ) 746 Influence of Wechat Platform Continuous Nursing on the Quality of Life of Cancer Patients LUO Jing, LIU Wen-zhuo, NIE Peng-min, et al DOI: 10.3969/j.issn.1671-7171.2020.05.034 【Objective】 To analyze the influence of wechat platform continuous nursing on the quality of life and psychological status of cancer pain patients.【Methods】 The clinical data of 240 patients with advanced cancer pain admitted to our hospital from July 2016 to July 2019 were analyzed retrospectively. According to the different ways of follow-up nursing after discharge, they were divided into observation group (wechat platform continuous nursing) and control group (telephone continuous follow-up nursing), 120 cases in each group. EORTC QLQ-C30, SAS, SDS and NRS were used to assess the quality of life of cancer patients. The quality of life, anxiety, depression and pain tolerance of the two groups were compared before and after nursing. 【Results】 The scores of body, role, emotion, society and cognitive function in the observation group were higher than those in the control group, and the scores of fatigue, pain, nausea and vomiting were lower than those in the control group (P<0.05). The scores of dyspnea, economic difficulty, insomnia, loss of appetite, constipation and diarrhea in the observation group were lower than those in the control group (P<0.05). The incidence rate of mild anxiety, moderate anxiety, severe anxiety and depression in observation group was lower than that in control group, the difference was statistically significant. The NRS score of the observation group was lower than that of the control group, and the satisfaction rate of return visit and health education was higher than that of the control group (P<0.05).【Conclusion】 Wechat platform continuous nursing can effectively alleviate the pain of cancer pain patients, improve the quality of life and satisfaction of cancer pain patients 2020 Vol. 37 (5): 746-748 [Abstract] ( 130 ) HTML (1 KB)  PDF  (0 KB)  ( 9 )

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