免疫抑制模型大鼠脑脊液中两种新型隐球菌感染的检测方法比较

doi:10.3969/j.issn.1671-7171.2020.05.005

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Abstract 【Objective】 Real-time fluorescent quantitative PCR (FQ-PCR) and ink staining were used to detect rat cerebrospinal fluid (CSF) in a rat model of Cryptococcus neoformans infection. The sensitivity and specificity of the two methods were compared to provide laboratory basis for clinical application. 【Methods】 Sixty male rats were randomly divided into four groups: group A and group C were injected with cyclophosphamide (150 mg/kg) once on the first day of the experiment, while group B and group D were injected with the same amount of 1 mL saline intraperitoneally. To establish infection model, rats in group A and group B were injected with 1 mL (containing 1×10⁶)of Cryptococcus neoformans suspension in the tail vein on the fourth day of the experiment, while rats in group C and group D received 1 ml saline as negative control. On the 6th day of the experiment, the cerebrospinal fluid of all rats was collected. Then Cryptococcus in cerebrospinal fluid of the four groups was detected by FQ-PCR and stained with ink at the same time. The results from the two methods were compared. 【Results】 The positive detection rate of Cryptococcus neoformans by FQ-PCR was higher than that of ink staining, and the difference was statistically significant (P<0.05). 【Conclusion】 FQ-PCR system is superior to ink staining in detecting Cryptococcus neoformans in cerebrospinal fluid of rats. It can be used for clinical detection of the presence of Cryptococcus neoformans infection in cerebrospinal fluid.

Key words： Cryptococcosis/DI Immunological Function Rats

Received: 10 October 2018

PACS:

R519.4

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2020.05.005 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2020/V37/I5/655

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