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Quick Search     Adv Search 2019 Vol. 36, No. 10 Published: 2019-10-30   1873 Relationship between Aminoacyl-tRNA Synthetases and Survival Probability of Lung Adenocarcinoma LI Yan, XIONG Yan-lu, SI Yue,et al DOI: 10.3969/j.issn.1671-7171.2019.10.001 【Objective】To explore the association between aminoacyl-tRNA synthetases and survival probability of lung adenocarcinoma via integrated analysis. 【Methods】We first obtained the gene expression data and clinical records of lung adenocarcinoma from the TCGA platform. Then we used GSEA analysis to explore the overall expression status of the aminoacyl-tRNA synthetase family in lung adenocarcinoma. Finally, we analyzed the relationship of the metabolic enzymes with total survival and progression-free survival of lung adenocarcinoma. 【Results】We found that the aminoacyl-tRNA synthetase family was highly expressed in lung adenocarcinoma. Survival analysis revealed that seven out of these enzymes were associated with overall survival of lung adenocarcinoma, and four enzymes were associated with progression-free survival. Patients with high expression of these enzymes showed worse prognosis. 【Conclusion】Aminoacyl -tRNA synthetases are highly expressed in lung adenocarcinoma and are closely related to survival probability. This provides a reference for understanding the metabolic characteristics and prevention methods of lung adenocarcinoma. 2019 Vol. 36 (10): 1873-1875 [Abstract] ( 122 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1876 Meta-analysis of Clinical Efficacy of Methylprednisolone Sodium Succinate for Sudden Deafness by Retroauricular Injection LIU Wen, HUANG Xiao-que DOI: 10.3969/j.issn.1671-7171.2019.10.002 【Objective】To evaluate the efficacy of retroauricular injection of methylprednisolone sodium succinate in the treatment of sudden deafness. 【Methods】Pubmed, Medline, CNKI, CBM, Weipu and Wanfang databases were retrieved electronically. Literatures that met the inclusion criteria were collected and data were extracted. RevMan 5.3 was used for meta-analysis. 【Results】14 literatures were included. Meta analysis showed that on the basis of routine treatment, the curative effect of retroauricular injection group was better than that of systemic hormone group, with RR (95% CI) of 1.29 (1.19, 1.40); the curative effect of retroauricular injection group was better than that of routine treatment except systemic hormone, with RR (95% CI) of 1.34 (1.10, 1.64); and the effect of systemic glucocorticoid therapy was better than that of systemic hormone therapy for sudden deafness. After injection of methylprednisolone sodium succinate, the curative effect was higher than that of the conventional treatment group, its RR (95% CI) was 1.13 (1.02, 1.26). 【Conclusion】Retroauricular injection of methylprednisolone sodium succinate is effective in the treatment of sudden deafness, which is worthy of promotion in clinical practice. 2019 Vol. 36 (10): 1876-1880 [Abstract] ( 135 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1881 Analysis of Cardiovascular Malformations and Pregnancy Outcomes in 112 Fetuses of Single Umbilical Artery LI Qiao-rong, LONG Xiang-dang, YAO Sui,et al DOI: 10.3969/j.issn.1671-7171.2019.10.003 【Objective】To investigate the clinical features of single umbilical artery (SUA) with cardiovascular malformations. 【Methods】A total of 112 fetuses with SUA were retrospectively analyzed. Clinical data and ultrasound images were collected to investigate complication with cardiovascular malformations and pregnancy outcomes. 【Results】Among the 112 SUA patients, simple SUA occurred in 80 cases(71.4%) and SUA complicated with other structural malformations were seen in 32 cases (28.6%),of which 25 cases(25/32,22.3%) were combined with cardiovascular malformations including 18 cases of severe complex malformations. No abnormal chromosome findings were shown in all 25 cases of SUA with cardiovascular malformations. However,20 cases were induced labor and 5 live births (2 cases of ventricular septal defect and 3 cases of ventricular septal defect with left superior vena cava were diagnosed by prenatal and postnatal ultrasound follow-up). The cardiovascular malformations in the top three places were ventricular septal defect (17/25, 68%), persistent left superior vena cava (6/25, 24%) and persistent arterial trunk (5/25, 20%). 【Conclusions】The most common malformations in SUA is cardiovascular malformation, which is so complicated types and poor prognosis. The diagnosis of fetal structural abnormalities during pregnancy, especially in the second trimester, is important for early intervention and reduction of birth defects. 2019 Vol. 36 (10): 1881-1883 [Abstract] ( 161 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1883 Clinical Effect of Traditional Occlusion Therapy Combined with Comprehensive Training on Ametropia Amblyopia Children Aged 4~8 Years Old XIANG Jian-bo, YANG Jun-fang, GUO Yan, et al DOI: 10.3969/j.issn.1671-7171.2019.10.004 【Objective】To investigate the clinical effect of traditional occlusion therapy combined with comprehensive training on ametropia amblyopia children aged 4~8 years old. 【Methods】A total of 84 children (aged 4~8 years old) with ametropia amblyopia in our hospital from January 2016 to February 2018 were selected. According to the random number table method, the patients were divided into the control group and the observation group, with 42 cases in each group. The children in the control group received traditional occlusion therapy; the children in the observation group received traditional occlusion therapy combined comprehensive training. The therapeutic effects of the two groups were compared. 【Results】Before treatment, there was no significant difference in the visual acuity between the two groups (P>0.05). After treatment, the visual acuity of the ametropia amblyopia children in the observation group was better than that in the control group, and the difference was statistically significant (P<0.05). The effective rate of treatments in children with ametropia amblyopia in the observation group was 95.24%, which was higher than that in the control group 80.95%, and the difference was statistically significant (P<0.05). The treatment satisfaction of 4~8 year-old ametropia amblyopia children in the observation group was 100%, which was higher than that in the control group (88.10%); the difference was statistically significant (P<0.05). 【Conclusion】Traditional occlusion therapy combined with comprehensive training in children with ametropia amblyopia aged 4 to 8 years has an ideal overall therapeutic effect, which can improve the vision of children with glasses and is simple to operate and worthy of clinical application. 2019 Vol. 36 (10): 1883-1885 [Abstract] ( 105 ) HTML (1 KB)  PDF  (0 KB)  ( 9 ) 1886 Hyaluronic Acid Mediated Nano Silver Targeted Liposome to Promote Burn Wound Healing WANG Rui, TANG Zhi-shui, PENG Hui-zi,et al DOI: 10.3969/j.issn.1671-7171.2019.10.005 【Objective】To study the effect of hyaluronic acid (HA) mediated nano silver targeted liposomes on burn wound healing, and to explore the ratio of HA to Ag sulfadiazine.【Methods】The formulation includes 100% sulfadiazine silver (control formulation), 95% sulfadiazine silver + 5% hyaluronic acid (formulation a), 90% sulfadiazine silver + 10% hyaluronic acid (formulation b). 54 SD rats with deep second degree burn were randomly divided into control group, experimental group A and experimental group B, with 18 rats in each group. Six rats in each group were killed on d7 and d14 after the application of control formula, formula A and formula B respectively. The changes of hydroxyproline, collagen ratio and DNA proliferation cycle of dermal cells were measured.The time of wound healing and the area of residual wound were observed.【Results】The content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group d7 were higher than that of group d3. The content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group A were higher than that of group A. the content of hydroxyproline and the proportion of type Ⅲ/Ⅰ collagen in the wound tissue of group B were higher than that of group A (P<0.05). The DNA in G0/G1 phase of dermal cells in d5, d7, d9 and d11 group was significantly lower than that in d3 group, and that in S phase was significantly higher than that in d3 group (P<0.05). The proportion of DNA in G0/G1 phase of d5, d7 and d9 dermal cells in the group A was lower than that in the control group, and the proportion of DNA in G0/G1 phase of d11 dermal cells in the group B was higher than that in the group A; the proportion of DNA in G0/G1 phase of d5 and d7 dermal cells in the group B was lower than that in the group A, and the proportion of DNA in G0/G1 phase of d9 and d11 dermal cells in the group B was higher than that in the group A (P<0.05). The area of residual wound in the group A was lower than that in the control group. The area of residual wound in the group B was lower than that in the group A (P<0.05). The average healing time of the control group was (14.46±1.17) days, higher than that of the experimental group A (12.87±0.75) days, and the average healing time of the experimental group A was higher than that of the experimental group B (11.87± 0.55 days), the difference was statistically significant (P<0.05). 【Conclusion】Hyaluronic acid mediated nano silver targeting liposomes can effectively promote wound healing,and the combination of 90% sulfadiazine and 10% hyaluronic acid is ideal for promoting wound healing. 2019 Vol. 36 (10): 1886-1890 [Abstract] ( 132 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1890 Correlation between Sleep Structure Abnormalities and Cognitive Function Scores (MMSE and MoCA) in Patients with Alzheimer's Disease LI Huai-su, ZHOU Wen, ZENG Tian-fang,et al DOI: 10.3969/j.issn.1671-7171.2019.10.006 【Objective】To investigate the relationship between sleep structure abnormalities and cognitive function in patients with Alzheimer's disease. 【Methods】A total of 100 patients with Alzheimer's disease who were treated in our hospital from January 2016 to December 2018 were enrolled, including 50 patients with senile dementia with sleep structural abnormalities (the observation group) and 50 patients with senile dementia without sleep structural abnormalities ( the control group). The changes in sleep parameters were compared between the two groups, and the cognitive function was assessed by using both Montreal Cognitive Assessment Scale (MoCA) and Mini-Mental State Examination (MMSE). The Spearman correlation was used to analyze the correlation between sleep structural abnormalities and cognitive function in patients with Alzheimer's disease. 【Results】There was no significant difference in the proportion of wake-up time, NREM 2 phase, and NREM 1+2 phase between the two groups (P>0.05). Compared to the control group, the proportion of NREM 1 phase and NREM phase in the observation group was significantly increased (P<0.05) and the proportion of the NREM 3+4 phase, REM, MMSE, and MoCA scores were significantly lower (P<0.05) in the observation group. The MMSE score of the observation group was positively correlated with NREM 3+ 4 phase, the nighttime minimum SaO2, and the average SaO2 at night (P<0.05), however, the MMSE score of the observation group was negatively correlated with NREM phase 1 + 2, ODI, AHI (P<0.05). There was no linear correlation of MMSE with REM phase and micro-awakening index (P>0.05). On the other hand, the MoCA score was positively correlated with the nighttime minimum SaO2 and nighttime average SaO2 (P<0.05), and negatively correlated with INREM 1 + 2 phase, oxygen saturation decline index (ODI) and apnea ventilation index (AHI) (P<0.05).The MoCA score was negatively correlated with micro-awakening index and ODI (P<0.05), and positively correlated with the REM phase (P<0.05), and no linearly correlated with the nighttime minimum SaO2 , the average SaO2 at night, the NREM 1 + 2 phase, and the NREM 3 + 4 Phase and AHI (P>0.05). 【Conclusion】Patients with Alzheimer's disease have abnormal sleep structure and decreased cognitive function. There is a correlation between sleep structure changes and cognitive function. 2019 Vol. 36 (10): 1890-1892 [Abstract] ( 147 ) HTML (1 KB)  PDF  (0 KB)  ( 35 ) 1893 Effects of Docetaxel Combined with Ifosfamide on Expression of Cyclin D1 and GADD153 in Ovarian Cancer CAOV3 Cells GE Qian-qian DOI: 10.3969/j.issn.1671-7171.2019.10.007 【Objective】To investigate the effects of docetaxel combined with ifosfamide on cyclin D1, growth inhibition and DNA damage-inducible protein 153 (GADD153) expression in ovarian cancer CAOV3 cells. 【Methods】The ovarian cancer CAOV3 cells were divided into three groups, which were recorded as blank control group, docetaxel group and combination group. Cells in the blank control group was routinely added with regular tissue culture medium. The docetaxel group was given single docetaxel intervention and the combination group was treated with docetaxel and ifosfamide. All treatments in three groups were applied for 72 hours. The cell proliferation rates at different time points were measured by MTT assay. The cell cycle was determined by flow cytometry with PI staining. The expression levels of cyclinD1 and GADD153 were detected by Western Blot. 【Results】 The cell proliferation rate of the combination group was higher than that of the docetaxel group and the blank control group at 24h, 36h and 72h (P<0.05). The G0/G1 phase of ovarian cancer CAOV3 cells in the combination group was lower than that in the docetaxel group and the blank control group; and the S phase in the combination group was higher than that in docetaxel group and blank control group (P<0.05). The level of cyclin D1 was lower but GADD153 was higher than those in the docetaxel group and the blank control group at 24h, 36h and 72h after intervention. P<0.05). 【Conclusion】Docetaxel combined with ifosfamide can induce and promote apoptosis of ovarian cancer CAOV3 cells through expression changes of cyclin D1 and GADD153 proteins. 2019 Vol. 36 (10): 1893-1895 [Abstract] ( 108 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1896 Clinical Study on Cytokine Detection of Peripheral Immune Injury in Patients with Lupus Nephritis LI Min-juan DOI: 10.3969/j.issn.1671-7171.2019.10.008 【Objective】 To investigate cytokines' expression and regulation of peripheral immune injury in patients with lupus nephritis (LN). 【Methods】A total of 56 patients with LN were selected as the observation group. LN patients were divided into the active group (≥10 points) and the stable group (<10 points) based on the results of the measurements using systemic lupus erythematosus (SLE) Disease Activity Scale. At the same time period, 34 healthy volunteers who admitted to our hospital for physical examination were enrolled as the control group. The levels of HMGB1, anti-C1q antibody, anti-AnuA antibody, CD3+, CD4+, CD8+ and ratio of CD4+/CD8+ in each group were determined. The correlations of blood HMGB1, anti-C1q and anti-AnuA level with T lymphocyte level were analyzed by SPSS Pearson analysis software.【Results】The levels of HMGB1, anti-C1q antibody and anti-AnuA antibody in LN active sub-group were higher than those in LN stable sub-group (P<0.05). The levels of CD3+, CD4+ and CD4+/CD8+ in LN active sub-group were lower than those in LN stable sub-group in observation group. The stable group (P<0.05), while the CD8+ level in the active sub-group was higher than that in the stable sub-group (P<0.05). SPSS Pearson correlation analysis showed that HMGB1, anti-C1q and anti-AnuA antibodies were negatively correlated with CD3+, CD4+ and CD4+/ CD8+ in LN patients (P<0.05),while they were positively correlated with CD8+ level (P<0.05). 【Conclusion】Blood HMGB1, anti-C1q antibody and anti-AnuA antibody are highly expressed in LN patients. HMGB1, anti-C1q antibody and anti-AnuA antibody have a correlation with T lymphocyte levels, which can participate in the occurrence and development of lupus nephritis. 2019 Vol. 36 (10): 1896-1898 [Abstract] ( 108 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1898 Effect of Propranolol on the Expression of VEGF and its Receptors VGFR1/VEGFR2 in Serum of Children with Hemangioma LI Lei, LIANG Wei, CHEN Chen,et al DOI: 10.3969/j.issn.1671-7171.2019.10.009 【Objective】To investigate the effect of propranolol on the expression of VEGF and its receptorsVGFR1/VEGFR2 in serum of children with hemangioma. 【Methods】From December 2014 to December 2017, 48 children with hemangioma in our hospital were selected. All the children were given propranolol orally (0.5 mg/kg for less than 3 months, 1 mg/kg for 3~6 months, 2 mg/kg for more than 6 months, twice a day, a total of 3 months). The levels of VEGF and its receptors VGFR1/VEGFR2 , were recorded before and 3 months after treatment. The tumor volume of the observation group before and 3 months after treatment was measured by ultrasound and the curative effect was evaluated. 【Results】 After one course of treatment, the levels of VEGF and its receptor VGFR1/VEGFR2 protein and mRNA expression in serum were significantly lower than those before treatment (P<0.05). The volume of hemangioma after treatment was (0.29 ± 0.09) cm3, which was significantly lower than that before treatment (0.78 ± 0.15) cm3, the difference was statistically significant (P<0.05). After treatment, Achauer method was used to evaluate the therapeutic effect, including 20 excellent cases, 15 good cases, 7 fair cases and 6 poor cases. The excellent and good rate was 72.9%.【Conclusion】The clinical effect of propranolol is better in children with hemangioma. The mechanism may be related to the down-regulation of serum VEGF and its receptor VGFR1/VEGFR2 expression levels. 2019 Vol. 36 (10): 1898-1900 [Abstract] ( 108 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1901 Evaluation of Left Ventricular Systolic Function in Children with Left Ventricular Noncompaction by Two-dimensional Speckle Tracking Imaging HU Yuan,LIU Qian-jun,XIAO Li-miao,et al DOI: 10.3969/j.issn.1671-7171.2019.10.010 【Objective】To investigate the value of two-dimensional speckle tracking imaging in the assessment of left ventricular systolic function in children with left ventricular noncompaction (LVNC). 【Methods】 A total of 27 patients with clinically diagnosed LVNC whose left ventricular systolic function were improved after standard anti-heart failure treatment were collected as the observation group. In addition, 27 healthy children were selected as the control group. The routine echocardiographic function parameters was examined firstly, then the left ventricular global longitudinal strain (GLS), global circumferential strain (GCS) and segmental longitudinal strain (LS) and circumferential strain (CS) were evaluated by two-dimensional speckle tracking imaging. The above parameters between LVNC children (pre-treatment and post-treatment) and the control group were compared. 【Results】After treatment, LVESV (left ventricular end-systolic volume) and LVEDD (Left ventricular end-diastolic diameter) in LVNC children were lower than those before treatment, while LVEF (left ventricular ejection fraction) was higher than that before treatment in LVNC children (all P<0.05). There were no significant differences in left ventricular end-diastolic volume (LVEDV) and LVEDD between the observation group post-treatment and the control group (P>0.05), while LVEF was significantly lower than the control group. After treatment, the left ventricular GLS, LS, GCS and CS of LVNC children were higher than those before treatment (all P<0.05). Compared to the control group, GLS, GCS, CS and 4 apical segments of LS decreased in post-treatment LVNC children (P<0.05),however, there was no significant difference of LS for the remaining segments between the control group and LVNC children post-treatment (P>0.05). In the post-treatment group of LVNC children, 25 patients had LVEF≥50% and the absolute value of LVEF increased by at least ≥10% compared with baseline. There were only 2 patients with LVNC had LVEF<50% and the absolute value of LVEF increased by <10% compared with baseline. During the follow-up period, 25 patients in the observation group kept LVEF ≥ 50% and the absolute value of LVEF increased by at least ≥ 10% compared with baseline; those two LVNC children with LVEF < 50% had no significant changes of the absolute value of LVEF compared with that before treatment. According to the modified ROSS classification, the cardiac function of the 27 HVNC children pre-treatment were grade Ⅳ. After treatment, the cardiac function of 19 children were grade II, 6 children were grade I, and 2 children were grade Ⅳ.【Conclusion】Left ventricular systolic function of children with LVNC get improved after standard anti-heart failure treatment, even the contraction of left ventricle segment does not return to normal level. Two-dimensional speckle tracking imaging can evaluate the left ventricular global and regional myocardial function pre- and post-treatment in children with LVNC, which provides useful reference for diagnosis and treatment of HVNC. 2019 Vol. 36 (10): 1901-1904 [Abstract] ( 118 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1904 Therapeutic Effect of Dendritic Cell-cytokine-induced Cellular Immunity Combined with TC Chemotherapy on Patients with Stage ⅡB-ⅣA Cervical Cancer JIAO Min, GUO Hui, WANG Shu-hong, et al DOI: 10.3969/j.issn.1671-7171.2019.10.011 【Objective】To investigate the efficacy of dendritic cell-cytokine-induced killer (DC-CIK) cell immunization combined with taxol plus carboplatin (TC) chemotherapy in patients with stage IIB-ⅣA cervical cancer. 【Methods】From September 2016 to August 2018, 72 patients with stage ⅡB-ⅣA cervical cancer in our hospital were selected and divided into observation group and control group with 36 cases in each group according to the random number table method. The control group was treated with TC chemotherapy, the observation group was treated with DC-CIK cell immunotherapy on the basis of the control group.Objective remission rate, disease control rate, Th1/Th2 secretion factor (IL-2, IL-10), PI3K / Akt signal transduction pathway index [phosphatidylinositol-3-kinase (PI3K), protein kinase B (Akt), amino terminal kinase 1 (JNK1)], osteopontin (OPN), endothelial glycoprotein (CD105) and the World Health Organization Quality of Life questionnaire summary score (WHOQOL-BREF) were compared between the two groups. 【Results】After 4 cycles of treatment, the objective remission rate and disease control rate of the observation group were 63.89% and 91.67%, respectively, which were significantly higher than 36.11% and 66.67% of the control group; IL-2 was higher than the control group, and IL-10 was lower than the control group; the levels of PI3K, Akt, JNK1, jnk2, JAK2, OPN and CD105 were lower than the control group, and the differences were statistically significant (P<0.05); the WHOQOL-BREF score of the observation group after 4 cycles of treatment was significant. It was higher than that of the control group (P<0.05).【Conclusion】DC-CIK cell immunity combined with TC chemotherapy in the treatment of ⅡB-ⅣA stage cervical cancer can inhibit tumor proliferation, invasion and metastasis, improve the immune function of patients, improve the quality of life of patients, and the effect is significant. The mechanism may be related to the inhibition of PI3K / Akt signal transduction pathway expression. 2019 Vol. 36 (10): 1904-1907 [Abstract] ( 86 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1908 Effect of Rehabilitation Training Before and After Operation on Urinary Control Function and Quality of Life of Patients Undergoing Radical Prostatectomy SU Jing-yi DOI: 10.3969/j.issn.1671-7171.2019.10.012 【Objective】To explore the effect of rehabilitation training before and after operation on urinary control function and quality of life of patients undergoing radical prostatectomy. 【Methods】From January 2016 to December 2017, 92 patients who were to undergo laparoscopic radical prostatectomy in our hospital were randomly divided into observation group and control group with 46 cases in each group. The control group was given routine nursing intervention combined with postoperative rehabilitation training, and the observation group was given routine nursing intervention combined with preoperative and postoperative rehabilitation training. The clinical symptoms, urodynamics, recovery control time, incidence of urinary incontinence and quality of life of the two groups were compared. 【Results】The total leakage times and the score of incontinence questionnaire in the observation group were significantly lower than those in the control group (P<0.05), and the leakage point pressure and the maximum closure pressure of Valsalva were significantly higher than those in the control group (P<0.05).The recovery time of urinary control function was significantly shorter than that of the control group (P<0.05), the incidence of postoperative urinary incontinence was significantly lower than that of the control group (P<0.05), the score of emotional status was significantly lower than that of the control group, and the score of social / family status and functional status was significantly higher than that of the control group (P<0.05).【Conclusion】Rehabilitation training before and after operation is helpful to relieve the clinical symptoms of radical prostatectomy, restore the function of urine control, reduce the incidence of urinary incontinence, and improve the quality of life of patients. 2019 Vol. 36 (10): 1908-1910 [Abstract] ( 87 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1911 Effect of microRNA-132 on Gene Expression Related to Retinal Neovascularization (RNV) in Human Retinal Microvascular Endothelial Cells (HRMECs) under Hypoxia/Reoxygenation (H/R) ZHANG Li-xin DOI: 10.3969/j.issn.1671-7171.2019.10.013 【Objective】To explore the effect of microRNA-132 on gene expression related to retinal neovascularization (RNV) in human retinal microvascular endothelial cells (HRMECs) under hypoxia/reoxygenation (H/R). 【Methods】HRMECs cell lines were cultured under hypoxia for 6 hours and then under normal oxygen for 6 hours as H/R group; anti-mir-132 was added as H/R + anti-mir-132 group under H/R condition; HRMECs were cultured under normal oxygen condition as negative control group. Total cell RNA was extracted and the changes of gene expression patterns were detected on the RNA microarray. Pathway analysis was performed using Rosetta Resolver 7.2 software package. Finally, candidate genes related to RNV were identified with Pubmed database. 【Results】Compared with the negative control group, there were significant changes in cell signals such as cell adhesion, adipocytokine and extracellular matrix receptor interaction in the H/R group (P<0.05). By inhibiting the expression of microRNA-132, we found that microRNA-132 participated in the regulation of various signal molecules in H/R cells. Three RNV related genes were screened out based on the analysis of Pubmed database: THBS1, IL-6 and CXCL8. 【Conclusion】Mir-132 participates in H/R-induced RNV by regulating the expression of multiple genes, which helps to further elucidate the mechanism of H/R-induced RNV. 2019 Vol. 36 (10): 1911-1915 [Abstract] ( 89 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1915 Analysis of Relationship between Treg/Th17 Cell Subsets in Peripheral Blood and Pregnancy Outcomes in Patients with Late Severe Preeclampsia LI Yan-jun, E Luo-ji DOI: 10.3969/j.issn.1671-7171.2019.10.014 【Objective】To investigate the relationship between Treg/Th17 cell subsets and pregnancy outcome in patients with later severe preeclampsia. 【Methods】A total of 120 patients with severe late preeclampsia were collected as the observation group and 40 normal late pregnant women with prenatal examination in the same period were selected as the control group. The contents of Treg and Th17 cells in peripheral blood were measured by flow cytometry. The results of pregnancy outcome, neonatal outcome and complications in the two groups were compared. 【Results】The level of Th17 cells in the peripheral blood of the observation group was significantly higher than that of the control group, and the level of Treg cells was significantly lower than that of the control group (P<0.05). The cesarean section rate in the observation group was significantly higher than that in the control group, and the natural delivery rate was significantly lower than that in the control group (P<0.05). The proportion of low birth weight infants in the observation group was significantly higher than that in the control group (P<0.05). The incidence of eclampsia in the observation group was significantly higher than that in the control group (P<0.05). 【Conclusion】 There are disorders of Treg and Th17 cell subsets in the peripheral blood of severe later preeclampsia patients. The cesarean section rate, the proportion of low birth weight infants and the incidence of eclampsia are significantly higher than those in normal pregnant women. 2019 Vol. 36 (10): 1915-1917 [Abstract] ( 81 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1918 Abnormal Glycosylation of Serum IgA1 in Children with Henoch Schonlein Purpura Correlated with Oxidative Stress QUAN Chun-mei, REN Zong-ming, WANG Neng-yong,et al DOI: 10.3969/j.issn.1671-7171.2019.10.015 【Objective】To investigate the abnormal glycosylation of serum IgA1 in children with Henoch Schonlein purpura(HSP)and reveal the correlation with oxidative stress. 【Methods】A total of 116 children with HSP admitted to our hospital included 79 cases of simple HSP (HSP group) and 37 cases of Henoch Schonlein purpura nephritis (HSPN group). Volunteers of 30 healthy children were used as the control group. The levels of abnormal glycosylation of serum IgA1, total serum IgA1,serum superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and advanced oxidation protein product (AOPPs) were measured and compared in the three groups, and their correlations were analyzed. 【Results】The serum IgA1 and abnormal glycosylation levels of IgA1 in the control group were lower than those in both HSP group and HSPN group, while the HSP group levels were lower than those of the HSPN group; the differences were statistically significant (P<0.05).The levels of SOD and GSH-PX in the control group were higher than those in the HSP group and the HSPN group; and the levels in the HSP group were higher than those in the HSPN group. The levels of MDA and AOPPs in the control group were lower than those in the HSP group and the HSPN group; and levels of the HSP group were lower than those of the HSPN group (P<0.05). Spearman analysis showed that IgA1 and the abnormal glycosylation of IgA1 were negatively correlated with SOD and GSH-PX (P<0.05), but positively correlated with MDA and AOPPs (P<0.05). 【Conclusion】The levels of IgA1 and abnormal glycosylation of serum IgA1 in children with HSP are high, which are significant correlation with oxidative stress. 2019 Vol. 36 (10): 1918-1920 [Abstract] ( 86 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1920 Clinical Efficacy of Minimally Invasive Thoracoscopic Surgery and Risk Factors of Postoperative Recurrence for Bilateral Pneumothorax with Spontaneous Pneumothorax XIA Mei, LI Wei-na, DU Gao-li,et al DOI: 10.3969/j.issn.1671-7171.2019.10.016 【Objective】To analyze the clinical efficacy and postoperative recurrent risk of minimally invasive thoracoscopic surgery in the treatment of bilateral pulmonary bullae with spontaneous pneumothorax. 【Methods】A total of 94 patients with bilateral pulmonary bullae and spontaneous pneumothorax, who were treated in our hospital from June 2016 to May 2017 were retrospectively analyzed. According to different surgical methods, patients were randomly divided into the observation group (n=50 cases) and the control group (n=44 cases). The control group received routine thoracotomy and the observation group received minimally invasive thoracoscopic surgery. The clinical efficacy and operative indicators of the two groups were compared. The postoperative complications and related risk factors of postoperative recurrence were analyzed between the two groups. 【Results】The total effective rate of the observation group was 94.00%, which was significantly higher than that of the control group 75.00% (P<0.05). The intraoperative blood loss of the observation group was less than that of the control group. The operation time, chest tube drainage time and hospitalization time in the observation group were shorter than those in the control group. The differences between the two groups were statistically significant (P<0.05). The incidence of complications in the control group was 18.19%, which was significantly higher than that of the observation group 4.00% (P<0.05). However, there was no significant difference in recurrence rates between the two groups (P>0.05). The incidence of recurrent pneumothorax and chest tube indwelling time more than 8 day in control group was significant higher than that in observation group (P<0.05). 【Conclusion】Minimally invasive thoracoscopic surgery improves the clinical efficacy of bilateral pulmonary bullae combined with spontaneous pneumothorax and reduces the incidence of postoperative complications. Our results suggests that recurrent pneumothorax and chest tube indwelling time >8d may be the main risk factors for postoperative recurrence. 2019 Vol. 36 (10): 1920-1922 [Abstract] ( 88 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1923 Effect of Sox9 Gene Expression on the Proliferation of Glioma Cells GAO Lei, FU Wen-sheng, NIE Ben,et al DOI: 10.3969/j.issn.1671-7171.2019.10.017 【Objective】To investigate the effect of testis determinant HMG box 9 (Sox9) gene expression on the proliferation of glioma cells.s. 【Methods】The brain glioma U87 cells were selected and randomly divided into control group, blank transfection group and interference group. The blank transfection group was transfected with lentivirus, the interference group was transfected with Sox9 lentivirus. MTT method was used to detect cell proliferation, DAPI nuclear staining was used to detect cell apoptosis, real-time fluorescent quantitative PCR was used to detect Sox9 mRNA expression, Western blot was used to detect Sox9, Sox2, nestin protein expression.【Results】The expression of Sox9 mRNA in the interference group was significantly lower than that in the blank transfection group and the control group, the blank transfection group was significantly lower than that in the control group (P<0.05), the apoptosis rate in the interference group was significantly higher than that in the blank transfection group and the control group, and the blank transfection group was significantly higher than that in the control group (P<0.05). The OD value of the interference group at 48 h and 72 h was significantly lower than that of the blank transfection group and the control group, and that of the blank transfection group at 48 h and 72 h was significantly lower than that of the control group (P<0.05). The relative expression of Sox9, Sox2 and nestin protein in the interference group was significantly lower than that in the blank transfection group and the control group, while the relative expression of Sox9 and sox27 protein in the blank transfection group was significantly lower than that in the control group (P<0.05).【Conclusion】Sox9 gene expression can inhibit the proliferation of glioma cells, promote apoptosis and down regulate the expression of Sox2 and nestin protein. 2019 Vol. 36 (10): 1923-1925 [Abstract] ( 111 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1926 Effect of Phloroglucinol Combined with Obucain on Cervical Softening Degree, VRS Score and Adverse Reactions in Patients Undergoing Hysteroscopy LUO Cai-quan, ZUO Guo-hui DOI: 10.3969/j.issn.1671-7171.2019.10.018 【Objective】To investigate the effect of phloroglucinol combined with obucain on cervical softening, VRS and adverse reactions in patients undergoing hysteroscopic surgery.【Methods】From March 2017 to March 2018, 124 patients undergoing hysteroscopic surgery in our hospital were randomly divided into control group and observation group, with 62 cases in each group. The patients in the observation group were treated with phloroglucinol on the basis of the control group. 【Results】The total effective rate of cervical softening in the observation group was 96.77% (60/62), significantly higher than that in the control group (77.42%) (P<0.05); after administration, the heart rate (HR) of the two groups increased, SpO2 decreased, and HR of the observation group was lower than that of the control group (P<0.05); the VRS score of the observation group was 64.52% (40/62), significantly higher than 33.87% (21/62) of the control group (P<0.05); the total adverse reaction rate of the observation group was 3.23% (2/62), significantly lower than 17.74% (11/62) of the control group (P<0.05).【Conclusion】The preoperative application of phloroglucinol combined with obuccain can improve the cervical softening, dilate the cervix, shorten the operation time, reduce the amount of bleeding, and have a positive impact on stabilizing vital signs, reducing pain, reducing adverse reactions and so on. 2019 Vol. 36 (10): 1926-1928 [Abstract] ( 84 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1929 Correlation Analysis of Imaging Features and Serum TSH, VEGF Levels in Patients with Thyroid Micro Carcinoma ZHANG Qing, ZHANG Yu-jun DOI: 10.3969/j.issn.1671-7171.2019.10.019 【Objective】To investigate the correlation between the imaging characteristics of thyroid micro carcinoma (TMC) and the levels of serum TSH and VEGF. 【Methods】From November 2015 to October 2018, 60 patients with TMC in our hospital were selected. Bravfv600e mutation was detected to be positive in all patients by thyroid papillary carcinoma gene test. The bilateral thyroid and cervical lymph nodes were scanned by Siemens S2000 ultrasound imager. The levels of VEGF and TSH in serum were measured by immunohistochemistry, and the bilateral thyroid and cervical lymph nodes in patients with different levels of VEGF and TSH were compared.【Results】In this study, the mean TSH level of 60 patients was (4.23 ± 1.58) mu / L, which was divided into high TSH group (≥4.23 mu /L) and low TSH group (＜4.23 mu/L).The results showed that there were significant differences in the maximum diameter, aspect ratio (A / T), boundary definition, membrane invasion and microcalcification between the two groups (P<0.05). According to the level of VEGF, they were divided into high VEGF group (≥ 27.34 U / ml) and low VEGF group (< 27.34 U / ml). The results showed that there were statistical differences in the maximum diameter of tumor,boundary clarity, membrane invasion and microcalcification between the two groups (P<0.05). The Pearson correlation of serum TSH and VEGF in TMC patients showed that the correlation coefficient of serum TSH and VEGF was 0.336, which was statistically significant (P<0.05).【Conclusion】The correlation between thyroid imaging characteristics of TMC and serum TSH, VEGF levels is mainly due to the common features of tumor benign and malignant judgment, so the combined detection of the two may improve the sensitivity and specificity of diagnosis. 2019 Vol. 36 (10): 1929-1931 [Abstract] ( 91 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1932 Expression of CAP2 Gene in HCC and its Prognostic Value LUO Li-hua, WANG Lan-qing, LUO Liu-fang,et al DOI: 10.3969/j.issn.1671-7171.2019.10.020 【Objective】To investigate the expression of CAP2 gene in HCC and its prognostic value.【Methods】The HCC related gene CAP2 was screened in Oncome database, and then the secondary analysis was carried out in Oncome and CCLE database, and the prognosis analysis was carried out in gepia database. 【Results】Based on Oncome database, the analysis of HCC miRNA microarray showed that CAP2 was up-regulated most significantly in HCC gene expression profile. A total of 412 different CAP2 studies were included in oncoming database, among which 33 were statistically significant, 14 were highly expressed, and 19 were low expressed. A total of 8 studies found that CAP2 was highly expressed in HCC (P<0.05), a total of 646 samples; then CCLE data was used to verify the expression level of CAP2 in HCC cells, and the results showed that CAP2 was highly expressed, consistent with oncomine. There was correlation between CAP2 and the disease-free survival rate of HCC patients (P<0.05), but not with the overall survival rate (P>0.05); the disease-free survival rate of patients with high expression of CAP2 was poor, and the disease-free survival rate of patients with low expression of CAP2 was better. 【Conclusion】CAP2 is highly expressed in HCC tissues and cells, and is related to the prognosis of HCC. It provides biomarkers for the new diagnosis and prognosis of HCC. 2019 Vol. 36 (10): 1932-1935 [Abstract] ( 94 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1935 Study on Correlation of CLIC1 Protein Expression with Invasion and Metastasis of Gastric Cancer WANG Zhi-qiang,SUN Peng, GAO Chun,et al DOI: 10.3969/j.issn.1671-7171.2019.10.021 【Objective】 To investigate the relationship of chloride intracellularchannel 1expression (CLIC1) with invasion, metastasis and prognosis of gastric cancer (GC). 【Methods】 The GC tissues and adjacent tissues were obtained from 94 patients. The expression of CLIC1 mRNA and protein were detected. The correlation of CLIC1 protein expression with gastric cancer clinical characteristics was analyzed. Multivariate Cox proportional model was applied to reveal the influencing factors of poor prognosis of gastric cancer. 【Results】 The positive expression rate of CLIC1 protein in GC adjacent tissues was 25. 0% (23/94), which was significantly lower than that of the GC tissues 86% (81/94) (P<0.01) .The positive expression of CLIC1 was not correlated with age, gender, tumor metastasis, however, it was related with tumor differentiation, lymph node metastasis, depth of invasion and TNM stage (all P<0.05). The expression level of CLIC1 mRNA in gastric cancer tissues was significantly higher than that in adjacent normal tissues (P<0.05). The median survival time of patients with low expression of CLIC1 protein was over 29 months, which was significantly longer than that of patients with positive expression for 25 months ( P<0.05). The 5-year survival rate of patients with low expression of CLIC1 protein was 63.9%, which was significantly higher than 20.7% of patients with positive expression of CLIC1 protein (P<0.05). GC invasion depth, lymph node metastasis, TNM stage and CLIC1 protein expression were independent prognostic factors for gastric cancer survivals (P<0.01). 【Conclusion】 CLIC1 plays an important role in the genesis and development of gastric cancer. High expression of CLIC1 is closely correlated with the invasion and metastasis of GC. 2019 Vol. 36 (10): 1935-1938 [Abstract] ( 86 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1939 Effect of Dexmedetomidine on Serum Inflammatory Factor Levels and Pulmonary Function in Patients with Laparoscopic Radical Resection of Rectal Cancer CAI Hao-liang, WANG Lan, CHEN Xi,et al DOI: 10.3969/j.issn.1671-7171.2019.10.022 【Objective】To investigate the effect of dexmedetomidine (Dex) on serum inflammatory factor levels and pulmonary function in patients with laparoscopic radical resection of rectal cancer. 【Methods】 A total of 100 patients with laparoscopic radical resection of rectal cancer were enrolled in the study. The control group contained 54 patients undergoing conventional anesthesia for the surgery, and the observation group included 46 patients with continuous intravenous infusion of Dex hydrochloride on the basis of conventional anesthesia. The respiratory rate (RR), heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), serum inflammatory factor levels (CRP, IL-6, TNF-α) and lung function parameters (Raw, MVV, FEV1, Cdyn) before the induction of anesthesia (T0), the establishment of artificial pneumoperitoneum (T1), the end of surgery (T2) were measured and compared between the two groups.【Results】There were no significant differences in terms of the above indexes at T0 between the two groups (P>0.05). The RR, HR, MAP and CVP of T1 and T2 in the control group were significantly higher than those the observation group (P<0.05). The CRP, IL-6 and TNF-α levels in the control group were significantly higher than those in the observation group at 24 h after operation(P<0.05). The MVV, FEV1 and Cdyn of the control group were significantly lower, while Raw was significantly higher than that of the observation group at 24 hours after surgery (P<0.01).【Conclusion】Dex can significantly reduce serum inflammatory factor levels in patients undergoing laparoscopic radical resection, stabilize heart rate, respiration and hemodynamics, thereby improving lung function and promoting clinical outcomes. 2019 Vol. 36 (10): 1939-1941 [Abstract] ( 94 ) HTML (1 KB)  PDF  (0 KB)  ( 14 ) 1942 Expression and Clinical Significance of Metastasis-associated in Colon Cancer-1 and KAI1/CD82 in Hepatocellular Carcinoma WANG Chun-rong DOI: 10.3969/j.issn.1671-7171.2019.10.023 【Objective】To explore the expression of MACC1 and KAI1/CD82 in hepatocellular carcinoma (HCC) patients , as well as the correlation with clinicopathological features. 【Methods】The expression levels of both MACC1 and KAI1/CD82 in 61 cases of HCC tissues and corresponding adjacent liver tissues were detected by immunohistochemistry, and the differences of MACC-1 and KAI1/CD82 protein expression between the two groups were compared. According to the clinical and pathological typing, the specimens of HCC radical resection group were grouped in order to reveal the difference of expression between the groups, and further explore the correlation between the expression of MACC-1 and KAI1/CD82 protein and clinical features. 【Results】Compared to adjacent tissues, MACC1 was significantly overexpressed in HCC tissues (P<0.05), while KAI1/CD82 was significantly down-expressed (P<0.05). The levels of MACC1 and KAI1/CD82 protein were correlated with tumor grade, lymph node metastasis, depth of invasion, and TNM stage (P<0.05). The overall survival time of patients with MACC1- or KAI1/CD82-positive HCC tumors was significantly longer than that of patients with MACC1 positive or KAI1 negative HCC (P<0.05). Importantly, multivariate analysis suggested that positive expression of either MACC1 or KAI1/CD82, as well as TNM stage, could be independent prognostic factors for overall survival in patients with HCC. 【Conclusion】 Abnormal expression of MACC1 and KAI1/CD82 plays an important role in the development of HCC. The combined detection of MACC1 and KAI1/CD82 is a biomarker for potential differential diagnosis of tumor metastasis and prognosis in HCC patients. MACC1 and KAI1/CD82 may represent promising metastatic and prognostic biomarkers, as well as potential therapeutic targets, for HCC. 2019 Vol. 36 (10): 1942-1944 [Abstract] ( 81 ) HTML (1 KB)  PDF  (0 KB)  ( 11 ) 1945 Emergency Laparoscopic Splenectomy for Traumatic Rupture of the Spleen GUO Yang, ZHANG Lu-yao, GAO Sheng,et al DOI: 10.3969/j.issn.1671-7171.2019.10.024 【Objective】To explore the clinical effect of emergency laparoscopic splenectomy (LS) in the treatment of traumatic splenic rupture. 【Methods】The clinical data of 82 patients with traumatic splenic rupture in our hospital were analyzed retrospectively. According to the operation method, they were divided into two groups: the observation group (LS operation,n=43) and the control group (exploratory laparotomy and total splenectomy,n=39). The operation time, intraoperative hemorrhage, postoperative exhaust time, hospitalization time and complications were compared between the two groups. The levels of WBC, IL-6, cor and TNF - α were compared before and 3 days after operation. 【Results】The operation time of the observation group was longer than that of the control group, and the postoperative exhaust time, average hospitalization time and intraoperative bleeding volume of the observation group were less than those of the control group (P<0.05). The level of Cor, TNF -α, IL-6 water in the two groups was higher than that before operation on the 3rd day after operation, and the WBC level in the control group was higher than that before operation on the 3rd day after operation, the difference was statistically significant (P<0.05); the level of Cor, TNF -α, IL-6, WBC in the observation group was lower than that in the control group on the 3rd day after operation, the difference was statistically significant (P<0.05). The incidence of complications in the observation group was 6.98% (3/43) lower than that in the control group 23.08% (9/39), the difference was statistically significant (χ2=-6.270, P<0.05).【Conclusion】Patients with traumatic splenic rupture after LS have less trauma, less complications, less stress and inflammatory response, and quick recovery, which is worthy of clinical application. 2019 Vol. 36 (10): 1945-1947 [Abstract] ( 90 ) HTML (1 KB)  PDF  (0 KB)  ( 13 ) 1948 Effect of Transesophageal Echocardiography Capacity Guidance Rehydration on Renal Function during Laparoscopic Radical Cystectomy FEI Hong-liang, LI Rong-fu, YU Hong,et al DOI: 10.3969/j.issn.1671-7171.2019.10.025 【Objective】To investigate the effect of transesophageal echocardiography (TEE) capacity guidance rehydration on the renal function indexes macroglobulin (β2-MG), proteinuria and urinary creatinine (UCr) in patients undergoing laparoscopic radical cystectomy of bladder cancer. 【Methods】A total of 160 patients with bladder cancer were enrolled. They were randomly divided into the TEE capacity guidance rehydration treatment group (observation group) and the conventional rehydration treatment group (control group), with 80 cases in each group. Data of cardiac function, major renal function index and postoperative complications in patients were recorded and compared in the tow groups. 【Results】There were no significant differences in the above indexes between the two groups before operation (P>0.05). Compared to the control group, the levels of renal function indexes β2-MG, BUN, SCr, UCr and 24h urine protein in the observation group were much lower; the differences were statistically significant (P<0.05). The hospitalization time of the observation group was (11.36±1.47)d, which was significantly less than that of the control group (16.23±1.51)d, and the difference was significant (P<0.05). The incidence of early complications and late complications were significantly lower in the observation group than in the control group (P<0.05).【Conclusion】TEE capacity guidance rehydration therapy can effectively improve postoperative renal function and reduce postoperative complications. 2019 Vol. 36 (10): 1948-1950 [Abstract] ( 86 ) HTML (1 KB)  PDF  (0 KB)  ( 12 ) 1951 Application of Preoperative Abdominal Breathing Training in Elderly Patients undergoing Non-anesthetic Colonoscopy GUO Yang, ZOU Jing DOI: 10.3969/j.issn.1671-7171.2019.10.026 【Objective】To explore the application of preoperative abdominal breathing training in elderly patients undergoing non-anesthetic colonoscopy. 【Methods】A total of 200 patients with colorectal cancer who were hospitalized for non-anesthetic colonoscopy from September 2017 to June 2018 were randomly divided into the control group and the intervention group with 100 cases in each group. The control group received routine administration, while the intervention group was guided for abdominal breathing training three days before the examination. The degree of pain (Facial Expression Pain Scale, FPS-R) and anxiety (Self-Assessment Scale, SAS) plus changes of blood pressure and heart rate before and during examination were compared between the two groups. 【Results】The score of FPS-R scale in the intervention group was lower than that in the control group (P<0.05). The systolic blood pressure, diastolic blood pressure and heart rate in the two groups during colonoscopy were lower than those before the examination (P<0.05); the systolic blood pressure, diastolic blood pressure and heart rate in the intervention group during colonoscopy were lower than those in the control group (P<0.05). SAS score of the intervention group before colonoscopy procedure was lower than that of the control group. The difference was statistically significant (P<0.05). 【Conclusion】Preoperative abdominal breathing training has a good effect on elderly patients undergoing non-anesthetic colonoscopy. It reduces pain, stress and anxiety. 2019 Vol. 36 (10): 1951-1953 [Abstract] ( 87 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1954 Retrograde Ureteroscopic Lithotripsy for 122 Cases of Renal Calculi ≤ 2 cm WEI Qiang, YAN Xiao-jing, YU Guang-yang,et al DOI: 10.3969/j.issn.1671-7171.2019.10.027 【Objective】To investigate the efficacy and safety of retrograde ureteroscopic lithotripsy (RIRS) in the treatment of renal calculi with diameter less than or equal to 2 cm.【Methods】From January 2015 to January 2019, 245 cases of renal calculi with diameter less than or equal to 2 cm were analyzed retrospectively. According to the different operation methods, they were divided into the RIRS group (n=122) and the PCNL group (n=123). The operation related indexes, stone finding rate, one week clearance rate, final stone clearance rate and complication rate were compared between the two groups. 【Results】The average operation time and postoperative hospitalization time of the RIRS group were shorter than that of the PCNL group, and the average lithotripsy time was longer than that of the PCNL group (P<0.05). One week stone clearance rate of the RRIS group was lower than that of the PCNL group (P<0.05). There was no significant difference between the two groups in stone finding rate and final stone clearance rate (P>0.05). The incidence of postoperative complications in RIRS group was 5.74% (7 / 122) lower than that in PCNL group (23.58%) (29 / 123), the difference was statistically significant (χ2=5.942,P=0.021<0.05).【Conclusion】RIRS can achieve the same effect as PCNL in the treatment of renal calculi ≤ 2 cm, but its safety is significantly higher than PCNL. It can be used as a surgical method for the treatment of renal calculi ≤ 2 cm in diameter. 2019 Vol. 36 (10): 1954-1956 [Abstract] ( 80 ) HTML (1 KB)  PDF  (0 KB)  ( 10 ) 1957 Relationship between Serum Uric Acid and Homocysteine of Elderly Patients with Heart Failure Preserved by Ejection Fraction ZHANG Hua, WANG Meng, GAO Hua,et al DOI: 10.3969/j.issn.1671-7171.2019.10.028 【Objective】To explore the relationship between the levels of serum uric acid (BUA) and homocysteine (Hcy) and the severity of the disease and prognosis in the elderly patients with heart failure (HFPEF) preserved by ejection fraction.【Methods】A total of 203 elderly HFPEF patients (observation group) admitted to our hospital from March 2016 to September 2017 were divided into two groups according to NYHA cardiac function grade Ⅱ (n=72), cardiac function grade Ⅲ (n=75) and cardiac function grade Ⅳ (n=56). Meanwhile, 50 healthy volunteers in the same period were selected as the control group. BUA, Hcy, NT-proBNP and LVEF were compared in each group. One year follow-up, according to the prognosis, patients were divided into poor prognosis group and good prognosis group. ROC curve was used to analyze the prognostic value of BUA and Hcy. 【Results】The levels of BUA, Hcy and NT-proBNP in the observation group were significantly higher than those in the control group, the difference was statistically significant (P<0.05); compared with levf, the difference was not statistically significant (P>0.05). The levels of BUA, Hcy and NT-proBNP were the highest in grade Ⅳ group, the second in grade Ⅲ group, and the lowest in grade Ⅱ Group (P<0.05). Pearson analysis showed that serum BUA and Hcy levels were positively correlated with NT-proBNP (r=0.784 and 0.732, respectively, P<0.05). 203 patients were hospitalized or died due to adverse mace events (poor prognosis group), including 27 cases of central colic, 19 cases of acute myocardial infarction, 13 cases of serious arrhythmia and 5 cases of death. The other 139 patients were included in the good prognosis group. The proportion of NYHA Ⅲ~Ⅳ, serum BUA, Hcy and NT-proBNP water in patients with good prognosis were lower than those in patients with poor prognosis (P<0.05).【Conclusion】The level of serum BUA and Hcy in HFPEF patients increases significantly, which is positively correlated with left ventricular function, and has a certain reference value for the diagnosis, severity and prognosis of HFPEF. 2019 Vol. 36 (10): 1957-1959 [Abstract] ( 84 ) HTML (1 KB)  PDF  (0 KB)  ( 14 )

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