Abstract：【Objective】To investigate the effects of ezetimibe on cardiac function and the phosphatidylinositol 3-kinase/protein kinase B/glycogen synthase kinase 3β(PI3K/AKT/GSK3β) pathway after acute myocardial infarction in hyperlipidemic rats. 【Methods】Fifty successfully modeled hyperlipidemic rats were randomly divided into a high-fat sham surgery group (n=16), a high-fat AMI group (n=17), and an ezetimibe group (n=17). Eight rats were randomly selected and fed with regular feed (normal control group). The high-fat AMI group and ezetimibe group rats underwent coronary artery ligation surgery to establish AMI models (the high-fat sham surgery group only had suture without ligation). 48 hours after surgery, there were 10, 9, and 9 surviving rats in the high-fat sham surgery group, high-fat AMI group, and ezetimibe group, respectively. We compared the levels of left ventricular end systolic volume (LVESV), left ventricular end diastolic diameter (LVEDV), and left ventricular ejection fraction (LVEF) in each group of rats after gastric lavage, observed the pathological changes in rat myocardial tissue, compared the apoptosis index (AI) of myocardial cells in each group, and compared the changes in PI3K, p-PI3K, AKT, p-AKT, GSK3β, and p-GSK3β protein levels in myocardial tissue. 【Results】Compared with the normal control group and high-fat sham surgery group, the levels of LVEDV and LVESV increased (P<0.05) and LVEF decreased in the high-fat AMI group and ezetimibe group rats(P<0.05); The LVEDV and LVESV levels in the ezetimibe group were lower than those in the high-fat AMI group (P<0.05), and the LVEF level was higher than that in the high-fat AMI group (P<0.05). Compared with the high-fat AMI group rats, the pathological changes in the myocardial tissue of the ezetimibe group rats were improved. Compared with the normal control group and the high-fat sham surgery group, the AI levels of myocardial cells in the high-fat AMI group and the ezetimibe group were increased (P<0.05); The AI level of myocardial cells in the ezetimibe group was lower than that in the high-fat AMI group (P<0.05). Compared with the normal control group and high-fat sham surgery group, the levels of p-PI3K/PI3K, p-AKT/AKT, and p-GSK3β/GSK3β in the myocardial tissue of rats in the high-fat AMI group and ezetimibe group were all reduced (P<0.05); The levels of p-PI3K/PI3K, p-AKT/AKT, and p-GSK3β/GSK3β in the myocardial tissue of rats in the ezetimibe group were higher than those in the high-fat AMI group (P<0.05). 【Conclusion】Ezetimibe may inhibit myocardial cell apoptosis by promoting the activation of the PI3K/AKT/GSK3β pathway, and exert a protective effect on cardiac function in hyperlipidemic rats after AMI.
延云鹏, 王淼. 依折麦布对高脂血症大鼠急性心肌梗死后心功能及PI3K/AKT/GSK3β信号通路的影响[J]. 医学临床研究, 2024, 41(8): 1164-1167.
YAN Yunpeng, WANG Miao. The Effect of Ezetimibe on Cardiac Function and PI3K/AKT/GSK3 β Signaling Pathway after Acute Myocardial Infarction in Hyperlipidemic Rats. JOURNAL OF CLINICAL RESEARCH, 2024, 41(8): 1164-1167.
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2024, Vol. 41 
