锦红汤对多重耐药铜绿假单胞菌感染大鼠肠道屏障功能的影响

doi:10.3969/j.issn.1671-7171.2023.04.005

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摘要【目的】探讨锦红汤对多重耐药铜绿假单胞菌(MDRPA)感染大鼠肠道屏障功能的影响。【方法】从65只大鼠中随机抽取10只仅生理盐水0.5 mL灌胃,设为正常组;其余55只建立肠道MDRPA感染大鼠模型,建模成功50只,随机分为模型组、锦红汤低剂量组、锦红汤中剂量组、锦红汤高剂量组、哌拉西林钠他唑巴坦钠组(TZP组),每组10只,其中锦红汤低、中、高剂量组分别以生药量3.2 g/kg、6.3 g/kg、12.6 g/kg锦红汤浓缩液灌胃,TZP组以TZP 70 mg/kg灌胃,各组给药容积均为10 mL/kg,连续干预2周;正常组、模型组给予等容量生理盐水灌胃。比较各组肠道屏障功能、回肠内容物细菌数量及结肠组织中Toll样受体4(TLR4)、髓样分化因子88(MyD88)、核转录因子κB p65(NF-κB p65)、p-NF-κB p65蛋白表达水平。【结果】与正常组比较,模型组血清二胺氧化酶(DAO)、内毒素(ET)水平及回肠内容物细菌计数均升高(P<0.01);与模型组比较,TZP组和锦红汤各剂量组血清DAO、ET水平及回肠内容物细菌计数均降低(P<0.05),且锦红汤各剂量组呈量效关系。与正常组比较,模型组TLR4、MyD88蛋白表达量及p-NF-κB p65/NF-κB p65升高(P<0.01);与模型组比较,TZP组和锦红汤各剂量组TLR4、MyD88蛋白表达量及p-NF-κB p65/NF-κB p65均降低(P<0.05),且锦红汤各剂量组呈量效关系。【结论】锦红汤可改善MDRPA感染大鼠肠道屏障功能,降低回肠细菌计数,其可能通过抑制TLR4/MyD88信号通路来发挥作用。

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	向琳
	王生辉

关键词 ：铜绿假单胞菌, 抗药性,微生物, 疾病模型,动物, 大鼠

Abstract：【objective】 To explore the effect of Jinhong Decoction on intestinal barrier function in rats infected with multidrug-resistant Pseudomonas aeruginosa (MDRPA). 【Method】 A total of 10 rats were randomly selected from 65 rats and given only 0.5 mL of physiological saline by gavage as the normal group; The remaining 55 rats were used to establish a model of intestinal MDRPA infection,and 50 rats were successfully modeled. They were randomly divided into model group,low dose group of Jinhong Decoction,medium dose group of Jinhong Decoction,high dose group of Jinhong Decoction,and piperacillin sodium tazobactam sodium (TZP) group,with 10 rats in each group. The low,medium,and high dose groups of Jinhong Decoction were gavaged with crude drug doses of 3.2 g/kg,6.3 g/kg,and 12.6 g/kg of Jinhong Decoction concentrated solution,while the TZP group was gavaged with TZP 70 mg/kg,with a dose volume of 10 mL/kg for each group,Continuously intervened for 2 weeks; The normal group and model group were given equal volume of physiological saline by gavage. The expression levels of intestinal barrier function,bacterial count of ileal contents,Toll like receptor 4 (TLR4) in colon tissue,myeloid differentiation factor 88 (MyD88),nuclear transcription factor κB p65(NF-κB p65)and p-NF-κB p65 protein of each group were compared. 【Results】 Compared with the normal group,the model group showed elevated levels of serum diamine oxidase (DAO),endotoxin (ET),and bacterial count in ileal contents (P<0.01); Compared with the model group,the levels of serum DAO and ET,as well as the bacterial count of ileal contents in the TZP group and each dose group of Jinhong Decoction decreased (P<0.05),and there was a dose-effect relationship between each dose group of Jinhong Decoction. Compared with the normal group,the expression levels of TLR4 and MyD88 proteins and p-NF-κB p65/NF-κB p65 in the model group increased (P<0.01); Compared with the model group,the expression levels of TLR4 and MyD88 proteins and p-NF-κB p65/NF-κB p65 in the TZP group and Jinhong Decoction at different doses decreased (P<0.05),and there was a dose-effect relationship between each dose group of Jinhong Decoction.【Conclusion】 Jinhong Decoction can improve the intestinal barrier function and reduce the bacterial count in the ileum of MDRPA infected rats,which may play a role by inhibiting the TLR4/MyD88 signaling pathway.

Key words： Pseudomonas aeruginosa Drug Resistance,Microbial Disease Models,Animal Rats

收稿日期: 2022-06-15

中图分类号:

R378.991

通讯作者: * E-mail:wshsnk1385@163.com

引用本文:

向琳, 王生辉. 锦红汤对多重耐药铜绿假单胞菌感染大鼠肠道屏障功能的影响[J]. 医学临床研究, 2023, 40(4): 496-499.
XIANG Lin, WANG Sheng-hui. Effects of Jinhong Decoction on Intestinal Barrier Function in Rats Infected with Multidrug-resistant Pseudomonas Aeruginosa. JOURNAL OF CLINICAL RESEARCH, 2023, 40(4): 496-499.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2023.04.005 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2023/V40/I4/496

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