Abstract：【Objective】To investigate the effect of miR-133 expression on the growth of esophageal cancer cells.【Methods】The expression of miR-133 in esophageal carcinoma was detected by RT-PCR. miR-133 mimics, anti-mir-133 and their corresponding controls were transfected into esophageal cancer cells by Lipofectamine 2000. RT-PCR was used to detect the expression of miR-133, MTT was used to detect the cell proliferation, and Western blot was used to detect the expression of EGFR protein.【Results】 The relative expression of miR-133 in esophageal carcinoma tissues (0.33±0.08) was significantly lower than that in adjacent normal tissues (1.15±0.18). Overexpression of miR-133 significantly inhibited the growth of esophageal cancer cells, while down-regulation of miR-133 significantly enhanced the growth of tumor cells. Meanwhile, overexpression of miR-133 inhibited the expression of EGFR mRNA and protein, and down-regulation of miR-133 significantly enhanced the expression of EGFR mRNA and protein. Overexpression of EGFR reverses the inhibitory effect of miR-133 on the growth of esophageal cancer cells.【Conclusion】 miR-133 inhibits the growth of esophageal cancer cells by regulating the expression of EGFR.
苏霁清, 陈雯, 刘红兵, 张海明, 汪砥. 食管癌组织miR-133表达对其癌细胞生长的影响[J]. 医学临床研究, 2021, 38(1): 64-67.
SU Ji-qing, CHEN Wen, LIU Hong-bin, et al. Effect of miR-133 Expression on the Growth of Esophageal Cancer Cells. JOURNAL OF CLINICAL RESEARCH, 2021, 38(1): 64-67.
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2021, Vol. 38 
