Abstract:【Objective】 To investigate the efficacy of personalized 3D-printed surgical guide plate-assisted localization of hematoma puncture and drainage in patients with cerebral hemorrhage.【Methods】A total of 92 patients with cerebral hemorrhage were randomly divided into two groups, with 46 cases in each. The observation group underwent hematoma puncture and drainage with the assistance of a personalized 3D-printed surgical guide plate, while patients in the control group received CT-guided hematoma puncture and drainage. Surgical parameters, postoperative quality of life (measured by the SF-36 scale), neurological function [National Institutes of Health Stroke Scale (NIHSS) score], and incidence of postoperative rebleeding and secondary cerebral edema were compared between the two groups.【Results】The observation group had a shorter localization time and smaller residual hematoma volume compared with the control group (P<0.05). The rates of first-attempt puncture success, hematoma clearance, and correct drainage tube placement in the observation group were significantly higher than those in the control group (P<0.05). One week after surgery, the SF-36 total score in the observation group was higher than that in the control group (P<0.05), and at six months postoperatively, all SF-36 dimensions and total scores were significantly higher in the observation group (P<0.05). Six months after surgery, the NIHSS score of the observation group was lower than that of the control group (P<0.05). The incidences of postoperative rebleeding and secondary cerebral edema were significantly lower in the observation group (P<0.05).【Conclusion】Personalized 3D-printed surgical guide plate-assisted localization in hematoma puncture and drainage can effectively improve intraoperative localization accuracy, shorten localization time, increase first-attempt success rate, enhance neurological function and quality of life in patients with cerebral hemorrhage, and reduce postoperative complications. It is worthy of clinical application and promotion.