阿托伐他汀对慢性硬膜下血肿大鼠血管新生的作用及VEGF表达的影响

doi:10.3969/j.issn.1671-7171.2023.08.014

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摘要【目的】探讨阿托伐他汀对慢性硬膜下血肿(CSDH)大鼠血管新生的作用及对血管内皮生长因子(VEGF)表达的影响。【方法】30只SPF级SD大鼠随机分为假手术组、模型组、阿托伐他汀组,每组10只。阿托伐他汀组大鼠于CSDH模型建立成功后给予阿托伐他汀灌胃处理(连续7 d),假手术组、模型组于同时间灌胃等量生理盐水。采用免疫组化检测各组大鼠血肿外膜组织中微血管密度(MVD),实时荧光定量聚合酶链式反应(PCR)法检测各组大鼠脑组织中VEGF mRNA表达水平,采用免疫印迹法检测各组大鼠脑组织中VEGF蛋白水平。【结果】随着时间推移,模型组、阿托伐他汀组大鼠颅内血肿体积均小于第3天,且阿托伐他汀组小于模型组,差异有统计学意义(P<0.05)。与假手术组比较,模型组、阿托伐他汀组大鼠脑组织中MVD-CD34水平均升高(P<0.05),阿托伐他汀组低于模型组(P<0.05)。与假手术组比较,模型组、阿托伐他汀组大鼠脑组织中VEGF mRNA水平均升高,阿托伐他汀组低于模型组,差异有统计学意义(P<0.05)。与假手术组比较,模型组、阿托伐他汀组大鼠脑组织中VEGF蛋白水平均升高,阿托伐他汀组低于模型组(P<0.05)。【结论】阿托伐他汀可能通过下调VEGF表达抑制脑组织新生血管生成,进而抑制CSDH发生、发展。

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	李晶
	何晴
	黄波

关键词 ：血肿,硬膜下,慢性, 阿托伐他汀/药理学, 血管内皮生长因子类, 大鼠, 疾病模型,动物

Abstract：【Objective】To investigate the effect of atorvastatin on angiogenesis and the expression of vascular endothelial growth factor (VEGF) in chronic subdural hematoma (CSDH) rats. 【Methods】Thirty SPF grade SD rats were randomly divided into sham surgery group,model group,and atorvastatin group,with 10 rats in each group. After the successful establishment of the CSDH model,rats in the atorvastatin group were treated with atorvastatin by gavage for 7 consecutive days. The sham operated group and model group were gived with the same amount of physiological saline at the same time. Immunohistochemistry was used to detect microvascular density (MVD) in the outer membrane tissue of hematoma in rats of each group. Real time fluorescence quantitative PCR was used to detect the expression level of VEGF mRNA in the brain tissue of rats of each group. Immunoblotting was used to detect the changes in VEGF protein levels in the brain tissue of rats of each group. 【Results】As time passed,the volume of intracranial hematoma in the model group and atorvastatin group of rats was lower than that on day 3,and the atorvastatin group was lower than the model group,with a statistically significant difference (P<0.05). Compared with the sham surgery group,the levels of MVD-CD34 in the brain tissue of the model group and atorvastatin group were both increased (P<0.05),while the atorvastatin group was lower than the model group (P<0.05). Compared with the sham surgery group,the levels of VEGF mRNA in the brain tissue of the model group and atorvastatin group were both increased,while the atorvastatin group was lower than the model group,with a statistically significant difference (P<0.05). Compared with the sham surgery group,the levels of VEGF protein in the brain tissue of the model group and atorvastatin group were both increased,while the atorvastatin group was lower than the model group (P<0.05). 【Conclusion】Atorvastatin may inhibit neovascularization in brain tissue by down regulating VEGF expression,thereby inhibiting the occurrence and development of CSDH.

Key words： Hematoma,Subdural,Chronic Atorvastatin/PD Vascular Endothelial Growth Factors Rats Disease Models,Animal

收稿日期: 2022-05-13

中图分类号:

R651.155

引用本文:

李晶, 何晴, 黄波. 阿托伐他汀对慢性硬膜下血肿大鼠血管新生的作用及VEGF表达的影响[J]. 医学临床研究, 2023, 40(8): 1168-1171.
LI Jing, HE Qing, HUANG Bo. The Effect of Atorvastatin on Angiogenesis and VEGF Expression in Rats with Chronic Subdural Hematoma. JOURNAL OF CLINICAL RESEARCH, 2023, 40(8): 1168-1171.

链接本文:

http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2023.08.014 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2023/V40/I8/1168

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