PHOX2B在外周神经母细胞性肿瘤4种分型组织中的表达差异<sup>*</sup>

doi:10.3969/j.issn.1671-7171.2024.02.010

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摘要【目的】探讨同源异型盒蛋白2B(PHOX2B)在外周神经母细胞性肿瘤(pNTs)4种分型组织中的表达差异。【方法】回顾性分析2019年1月至2022年12月本院收治的65例pNTs患儿的临床资料,根据免疫组化综合评分方法对pNTs 4种分型中 PHOX2B的表达情况进行分析,采用受试者工作特征(ROC)曲线评估PHOX2B免疫组化综合评分预测神经母细胞瘤(NB)、节细胞性神经母细胞瘤(GNB)混杂型(GNBi)、节细胞性神经母细胞瘤结节型(GNBn)和节细胞性神经瘤(GN)的价值。【结果】男、女患儿病例数分别为33例、32例,无明显的性别差异;年龄分布主要为6岁以下患儿,其所占比例为75.38%(49/65);腹膜后为常见原发部位,其次为纵隔和肾上腺。PHOX2B在NB中免疫组化综合评分最高,其次为GNBn,GNBi和GN表达最弱,NB免疫组化综合评分显著高于GNBn、GNBi、GN,GNBn免疫组化综合评分显著高于GNBi、GN,差异均有统计学意义(P<0.05)。ROC曲线结果显示,PHOX2B免疫组化综合评分为5分时可作为诊断NB的参考阈值,PHOX2B免疫组化综合评分为3分可作为诊断GNBn的参考阈值(P<0.05)。【结论】PHOX2B在pNTs不同类型的表达有显著差异,其表达强度可作为pNTs病理分型的依据。

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	谭智文
	陈卫坚
	秦蕾
	郑台青

关键词 ：基因,同源盒, 神经母细胞瘤/病理学, 免疫组织化学

Abstract：【Objective】To investigate the differential expression of homologous heterojunction box protein 2B (PHOX2B) in four subtypes of peripheral neuroblastoma (pNTs) tissues. 【Methods】A retrospective analysis was conducted on 65 children with pNTs admitted to our hospital from January 2019 to December 2022. The expression of PHOX2B in four subtypes of pNTs was analyzed using the immunohistochemical comprehensive scoring method,The receiver operating characteristic (ROC) curve was used to evaluate the value of PHOX2B immunohistochemistry comprehensive score in predicting neuroblastoma (NB), ganglionic neuroblastoma (GNB) mixed type (GNBi), ganglionic neuroblastoma nodular type (GNBn), and ganglionic neuroma (GN). 【Results】The number of cases of male and female patients was 33 and 32 respectively, with no significant gender difference; The age distribution was mainly among children under 6 years old, accounting for 75.38%(49/65); The retroperitoneum was a common primary site, followed by the mediastinum and adrenal gland. PHOX2B had the highest comprehensive immunohistochemical score in NB, followed by GNBn, with the weakest expression of GNBi and GN. The comprehensive immunohistochemical score in NB was significantly higher than that of GNBn, GNBi, and GN. The comprehensive immunohistochemical score of GNBn was significantly higher than that of GNBi and GN, and the differences were statistically significant (P<0.05). The ROC curve results showed that a PHOX2B immunohistochemical comprehensive score of 5 could be used as a reference threshold for diagnosing NB, and a PHOX2B immunohistochemical comprehensive score of 3 could be used as a reference threshold for diagnosing GNBn (P<0.05).【Conclusion】There are significant differences in the expression of PHOX2B among different types of pNTs, and its expression intensity can serve as a basis for pathological classification of pNTs.

Key words： Genes, Homeobox Neuroblastoma/PA Immunohistochemistry

收稿日期: 2023-08-24

中图分类号:

R730.264.4

基金资助:*湖南省儿童医院课题基金项目(HNSET2021C05)

通讯作者: **E-mail: taiqing_zheng@163.com

引用本文:

谭智文, 陈卫坚, 秦蕾, 郑台青. PHOX2B在外周神经母细胞性肿瘤4种分型组织中的表达差异^*[J]. 医学临床研究, 2024, 41(2): 192-194.
TAN Zhiwen, CHEN Weijian, QIN Lei, et al. Differential Expression of PHOX2B in Four Subtypes of Peripheral Neuroblastoma Tissues. JOURNAL OF CLINICAL RESEARCH, 2024, 41(2): 192-194.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2024.02.010 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2024/V41/I2/192

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