Abstract：【Objective】To investigate the significance of the expression miR-223 and miR-155-5p in peripheral blood of patients with urinary sepsis after percutaneous nephroscope with holmium laser lithotripsy. 【Methods】Patients who underwent percutaneous nephroscope combined with holmium laser lithotripsy in our hospital from May 2015 to June 2020 were selected as the research objects. Fifty-two patients with urinary sepsis after the operation were taken as the observation group, and 52 patients without urinary sepsis were considered as the control group. The levels of conventional infection markers procalcitonin (PCT), C-reactive protein (CRP) and the expression of miR-223 and miR-155-5p in the two groups were compared. The influencing factors of urinary sepsis after percutaneous nephroscoped with holmium laser lithotripsy were analyzed, and the value of peripheral blood miR-223 and miR-155-5p expression in the diagnosis of postoperative urinary sepsis was evaluated. 【Results】In the observation group, the proportion of male patients, preoperative urine routine positive, operation time ＞ 90 min, and levels of peripheral blood PCT, CRP, miR-223 and miR-155-5p expression were all higher than those in the control group (P<0.05). Logistic regression analysis showed that male, preoperative urine routine positive, operation time ＞ 90 min, peripheral blood PCT, CRP levels, miR-223 and miR-155-5p expressions were all influential factors of urinary sepsis after percutaneous nephroscope combined with holmium laser lithotripsy (P<0.05). The expression of miR-223 and miR-155-5p in peripheral blood of patients with urinary sepsis after percutaneous nephroscope combined with holmium laser lithotripsy was positively correlated with the levels of conventional infection markers PCT and CRP (P<0.05). The ROC curve was drawn and it was found that the combined diagnosis of miR-223 and miR-155-5p in peripheral blood had the highest AUC value for postoperative urinary sepsisafter percutaneous nephrolithotripsy combined with holmium laser lithotripsy, which had a good diagnostic efficacy. 【Conclusion】Percutaneous nephroscope combined with holmium laser lithotripsy has high expression of miR-223 and miR-155-5p in peripheral blood of patients with urinary sepsis. Monitoring their levels after surgery is helpful for the early diagnosis and prognosis prediction of urinary sepsis, and reduces the mortality rate.
王佳, 段晓辉, 郝春林, 于磊, 袁建林, 刘飞, 秦卫军. 经皮肾镜钬激光碎石术后发生尿源性脓毒血症患者外周血miR-223、miR-155-5p表达量及相关性分析[J]. 医学临床研究, 2021, 38(11): 1621-1624.
WANG Jia, DUAN Xiao-hui, HAO Chun-lin, et al. Expression of miR-223 and miR-155-5p in Peripheral Blood of Patients with Urinary Sepsis after percutaneous Nephroscope with Holmium Laser Lithotripsy. JOURNAL OF CLINICAL RESEARCH, 2021, 38(11): 1621-1624.
2021, Vol. 38 
