摘要【目的】探讨血清miR-122水平对慢性丙型肝炎(CHC)患者肝纤维化程度的诊断价值。【方法】于本院感染科就诊并行肝穿刺活检的CHC患者120例,采用实时荧光定量聚合酶链反应(qRT-PCR)检测血清miR-122表达水平。采用受试者工作曲线(ROC)评估miR-122诊断CHC患者肝纤维化程度的价值。【结果】120例患者中,男62例,女58例,平均年龄(46.91±17.26)岁,丙氨酸转氨酶(ALT)水平为(72.85±21.34) U/L,天冬氨酸氨基转移酶(AST)水平为(76.31±18.43) U/L, miR-122表达水平为1.63±0.57,AST和血小板(PLT)比率指数(APRI)评分为(1.42±0.52)分。肝穿刺活检发现肝纤维化程度1/2/3/4级分别有37/41/28/14例,依据肝纤维化程度将患者分为:S1-2组和S3-4组两组。S3-4组AST、ALT、miR-122水平及炎症活动度显著高于S1-2组( P <0.05),而两组患者年龄、性别、APRI评分相比较差异无显著性( P >0.05)。miR-122预测CHC患者发生S3-4纤维化严重程度的最佳临界点为1.76。此时,miR-122预测灵敏度为78.91%,特异度为98.83%;ROC曲线下面积(AUC)为0.940。【结论】血清miR-122表达水平对CHC患者肝纤维化严重程度具有一定的诊断价值。
Abstract：【Objective】To explore the diagnostic value of serum microRNA-122 level in the degree of liver fibrosis in patients with chronic hepatitis C (CHC). 【Methods】One hundred and twenty patients with chronic hepatitis C in our hospital were enrolled. qRT-PCR was used to detect the expression of serum microRNA-122. The receiver operating characteristic (ROC) was used to evaluate the predictive value of microRNA-122 in the diagnosis of liver fibrosis in patients with chronic hepatitis C. 【Results】There were 62 males and 58 females with an average age of 46.91 ± 17.26 years.The levels of alanine aminotransferase(ALT)and aspartate aminotransferase (AST) were 72.85±21.34 U/L and 76.3±18.43 U/L, respectively.The expression level of miR-122 was 1.63±0.57, and the score of AST and platelet ratio index (APRI) was 1.42±0.52.Liver biopsy showed that there were 37/41/28/14 cases of liver fibrosis grade 1/2/3/4.According to the degree of liver fibrosis, the patients were divided into two groups: S1-2 group and S3-4 group. The levels of AST, alt, miR-122 and inflammatory activity in S3-4 group were significantly higher than those in S1-2 group ( P <0.05), but there was no significant difference in age, gender and APRI score between the two groups ( P >0.05).MiR-122 predicted that the best critical point of s3-4 fibrosis severity in CHC patients was 1.76.At this time, the predictive sensitivity of miR-122 was 78.91%, the specificity was 98.83%, and the area under the curve AUC was 0.940.【Conclusion】Serum microRNA-122 have diagnostic value for the severity of liver fibrosis in patients with CHC.
贾学友, 藏小晴. 血清miR-122水平对慢性丙型肝炎患者肝纤维化程度的诊断价值[J]. 医学临床研究, 2021, 38(3): 403-405.
JIA Xue-you, ZANG Xiao-qing. Diagnostic Value of Serum miR-122 Level in the Degree of Liver Fibrosis in Patients with Chronic Hepatitis C. JOURNAL OF CLINICAL RESEARCH, 2021, 38(3): 403-405.
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2021, Vol. 38 
