Abstract：【Objective】To detect the expression of circRNA_0006633 in liver cancer tissues and explore its biological regulation on cell proliferation, apoptosis and cell cycle.【Methods】Real-time quantitative polymerase chain reaction (RT-qPCR) was used to analyzed the expression level of circRNA_0006633 in liver cancer tissues and adjacent normal tissues, while the relationship between circRNA_0006633's expression and clinicopathological parameters was further determined. Then biological effects of circRNA_0006633 overexpression by lentivirus-mediated-plasmids (circ_0006633-pcDNA 3.1 plasmids)on cell proliferation, apoptosis, and cycle were investigated by RT-qPCR, western blot, CCK8 and flow cytometry.【Results】Compared to the paired adjacent normal tissues, the expression level of circRNA_0006633 in liver cancer tissues was lower. Furthermore, the low expression circRNA_0006633 was negatively correlated with survival outcome. Upregulation of circRNA_0006633 effectively reduced cell proliferation, increased apoptosis and arrested cells at G2/M phages, while the epithelial-mesenchymal transition (EMT)-related proteins were regulated as well.【Conclusion】Expression of circRNA_0006633 is lower in liver cancer tissues. circRNA_0006633 can inhibit the process of epithelial-mesenchymal transition pathway activation and reduce the proliferation of liver cancer cells, which provides new ideas for the treatment of liver cancer.
罗泽斌, 陈志安, 陈龙林, 杨贤杰, 郭国湖. 环状RNA circRNA_0006633 在肝癌组织中的表达及对肝癌细胞增殖能力的影响[J]. 医学临床研究, 2020, 37(1): 45-49.
LUO Ze-bin, CHEN Zhi-an,CHEN Long-lin,et al. Expression of CircRNA_0006633 in Liver Cancer Tissues and Its Effect on Cell Proliferation. JOURNAL OF CLINICAL RESEARCH, 2020, 37(1): 45-49.
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