Abstract：【Objective】To investigate the effects of docetaxel combined with ifosfamide on cyclin D1, growth inhibition and DNA damage-inducible protein 153 (GADD153) expression in ovarian cancer CAOV3 cells. 【Methods】The ovarian cancer CAOV3 cells were divided into three groups, which were recorded as blank control group, docetaxel group and combination group. Cells in the blank control group was routinely added with regular tissue culture medium. The docetaxel group was given single docetaxel intervention and the combination group was treated with docetaxel and ifosfamide. All treatments in three groups were applied for 72 hours. The cell proliferation rates at different time points were measured by MTT assay. The cell cycle was determined by flow cytometry with PI staining. The expression levels of cyclinD1 and GADD153 were detected by Western Blot. 【Results】 The cell proliferation rate of the combination group was higher than that of the docetaxel group and the blank control group at 24h, 36h and 72h (P<0.05). The G0/G1 phase of ovarian cancer CAOV3 cells in the combination group was lower than that in the docetaxel group and the blank control group; and the S phase in the combination group was higher than that in docetaxel group and blank control group (P<0.05). The level of cyclin D1 was lower but GADD153 was higher than those in the docetaxel group and the blank control group at 24h, 36h and 72h after intervention. P<0.05). 【Conclusion】Docetaxel combined with ifosfamide can induce and promote apoptosis of ovarian cancer CAOV3 cells through expression changes of cyclin D1 and GADD153 proteins.
葛倩倩. 多西他赛联合异环磷酰胺对卵巢癌CAOV3细胞cyclinD1及GADD153表达的影响[J]. 医学临床研究, 2019, 36(10): 1893-1895.
GE Qian-qian. Effects of Docetaxel Combined with Ifosfamide on Expression of Cyclin D1 and GADD153 in Ovarian Cancer CAOV3 Cells. JOURNAL OF CLINICAL RESEARCH, 2019, 36(10): 1893-1895.
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2019, Vol. 36 
