癌组织中SEPT9基因甲基化水平对Ⅱ~Ⅲ期可切除直肠癌术后无进展生存期的影响

doi:10.3969/j.issn.1671-7171.2024.03.015

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摘要【目的】探讨癌组织中SEPT9基因甲基化水平对Ⅱ~Ⅲ期可切除直肠癌患者术后无进展生存期的影响。【方法】129例均行R0全直肠系膜切除术治疗的Ⅱ~Ⅲ期可切除直肠癌患者,术后随访5年。检测并比较患者癌组织、癌旁组织中SEPT9基因甲基化阳性表达情况,分析患者癌组织中SEPT9基因甲基化水平与临床病理参数的关系,分析影响直肠癌术后复发或转移的因素,探讨癌组织中SEPT9基因甲基化阳性表达与患者术后无进展生存的关系。【结果】癌组织中SEPT9基因甲基化阳性表达率高于癌旁组织(P<0.05)。低分化、N₂期、Ⅲ期、肿瘤浸润脉管的可切除直肠癌患者癌组织中SEPT9基因甲基化阳性表达率分别高于中、高分化及N₀~N₁期、Ⅱ期、肿瘤未浸润脉管者(P<0.05)。Cox回归分析显示:术前癌胚抗原水平、病理分期、分化程度、SEPT9基因甲基化阳性是影响患者术后复发或转移的独立因素(P<0.05)。SEPT9基因甲基化阳性表达患者的无进展生存率低于阴性表达患者(P<0.05)。【结论】Ⅱ~Ⅲ期可切除直肠癌患者癌组织中SEPT9基因甲基化水平与临床病理参数及预后有关,SEPT9基因甲基化阳性表达患者术后复发、转移风险更高。

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	李曰平
	韩博
	张汝梦
	鞠倩

关键词 ：直肠肿瘤/外科学, DNA甲基化, 存活率

Abstract：【Objective】To investigate the impact of SEPT9 gene methylation level in cancer tissues on progression-free survival after surgery in patients with stage Ⅱ-Ⅲ resectable rectal cancer. 【Methods】A total of 129 patients who underwent R0 total mesorectal excision for stage Ⅱ-Ⅲ resectable rectal cancer were followed up for 5 years postoperatively. The positive expression of SEPT9 gene methylation in cancer tissues and adjacent non-cancerous tissues was compared, and the relationship of SEPT9 gene methylation levels in cancer tissues with clinicopathological parameters was analyzed. Factors affecting postoperative recurrence or metastasis of rectal cancer were investigated, along with the relationship between positive expression of SEPT9 gene methylation in cancer tissues and progression-free survival after surgery. 【Results】The positive expression rate of SEPT9 gene methylation in cancer tissues was higher than that in adjacent non-cancerous tissues(P<0.05). Patients with poorly differentiated, N₂ stage, stage Ⅲ, and tumor invasion into the vasculature had higher positive expression rates of SEPT9 gene methylation in cancer tissues than those with moderately to well-differentiated, N₀-N₁ stage, stage Ⅱ, and no tumor invasion into the vasculature(P<0.05). Cox regression analysis showed that preoperative carcinoembryonic antigen levels, pathological staging, differentiation degree, and positive SEPT9 gene methylation were independent factors affecting postoperative recurrence or metastasis(P<0.05). Patients with positive expression of SEPT9 gene methylation had lower progression-free survival rates than those with negative expression(P<0.05). 【Conclusion】The level of SEPT9 gene methylation in cancer tissues of patients with stage Ⅱ-Ⅲ resectable rectal cancer is related to clinicopathological parameters and prognosis. Patients with positive expression of SEPT9 gene methylation have a higher risk of postoperative recurrence and metastasis.

Key words： Rectal Neoplasms/SU DNA Methylation Survival Rate

收稿日期: 2023-10-25

中图分类号:

R735.37

通讯作者: *E-mail:86311370@qq.com

引用本文:

李曰平, 韩博, 张汝梦, 鞠倩. 癌组织中SEPT9基因甲基化水平对Ⅱ~Ⅲ期可切除直肠癌术后无进展生存期的影响[J]. 医学临床研究, 2024, 41(3): 375-378.
LI Yueping, HAN Bo, ZHANG Rumeng, et al. Effect of SEPT9 Gene Methylation Level in Cancer Tissue on postoperative Progression-free Survival in Patients with Stage Ⅱ-Ⅲ Resectable Rectal Cancer. JOURNAL OF CLINICAL RESEARCH, 2024, 41(3): 375-378.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2024.03.015 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2024/V41/I3/375

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