Abstract:【Objective】To explore the diagnostic efficacy of ultrasound-guided and CT-guided percutaneous lung biopsy for peripheral lung lesions. 【Methods】A retrospective analysis was conducted on the clinical data of 96 patients admitted to our hospital from March 2019 to March 2022 who were diagnosed with peripheral lung lesions through imaging examinations. They were divided into ultrasound-guided group and CT-guided group based on different guided biopsy methods, with 48 patients in each group. We compared the success rate, diagnosis rate, and pathological results of two groups of biopsies, as well as the total time, effective time, and average depth of puncture biopsy between the two groups. The incidence of complications during puncture biopsy were compared between the two groups. 【Results】There was no statistically significant difference in the success rate, diagnosis rate, and pathological results between the two groups (P>0.05). The total time, effective time, and average depth of puncture biopsy in the ultrasound-guided group were lower than those in the CT-guided group, with statistically significant differences (P<0.05). The total incidence of complications in the ultrasound-guided puncture biopsy group was 6.25% (3/48), which was lower than 20.83% (10/48) in the CT-guided group, with a statistically significant difference(χ2=4.360,P=0.037). 【Conclusion】 Both ultrasound-guided and CT-guided percutaneous lung biopsies have higher diagnostic efficacy for peripheral lung lesions, and the incidence of complications in ultrasound-guided percutaneous lung biopsy is relatively low.