基于TCGA数据库分析<em>CDKN2A</em>基因在结肠癌中的表达及其临床意义

doi:10.3969/j.issn.1671-7171.2024.12.006

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摘要【目的】基于肿瘤基因组图谱(TCGA)数据库分析CDKN2A基因在结肠癌中的表达及其临床意义。【方法】本研究收集肿瘤免疫评估数据库(TIMER)中的TCGA RNA-seq数据,分析CDKN2A在结肠癌中的表达情况及CDKN2A对结肠癌患者预后的影响,应用TIMER数据库检测免疫细胞浸润的情况,运用R软件包分析CDKN2A与临床特征的相关性,应用STRING数据库分析CDKN2A基因中蛋白互作网络信息。【结果】CDKN2A基因在16种肿瘤中被发现存在过表达现象,特别是在结肠癌中。CDKN2A表达水平较高的结肠癌患者预后较差,CDKN2A的表达与肿瘤T分期、N分期和病理分期相关(P<0.05)。受试者工作特征曲线分析显示,CDKN2A诊断结肠癌的效能较高,曲线下面积为0.918。CDKN2A与免疫检查点共表达结果显示,CDKN2A与CD274、CTLA4、HAVCR2、LAG3、PDCD1、PDCD1LG2、SIGLEC15、TIGIT共表达情况呈正相关(P<0.05)。Spearman相关性分析显示,CDKN2A表达情况与CCND1表达情况呈正相关(P<0.05)。【结论】CDKN2A在诊断结肠癌及评估患者预后方面具有较高效能,是治疗结肠癌的潜在靶点,对提高结肠癌的诊断准确性和评估预后具有重要意义。

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	汪欣宇
	王国清
	龚双喜

关键词 ：结肠肿瘤, 基因,p16, 数据库,核酸

Abstract：【Objective】To analyze the expression level and clinical value of CDKN2A gene in colon cancer based on TCGA database. 【Methods】In this study, TCGA RNA-seq data from the Tumor Immune Evaluation Database (TIMER) were collected to analyze the expression of CDKN2A in colon cancer and the impact of CDKN2A on the prognosis of colon cancer patients. The TIMER database was used to detect the infiltration of immune cells. The correlation between CDKN2A and clinical features was analyzed by R packet, and the protein interaction network information in CDKN2A gene was analyzed by STRING database. 【Results】CDKN2A gene was found to be overexpressed in 16 tumors, especially in colon cancer. The prognosis of colon cancer patients with higher CDKN2A expression level was worse, and the expression level of CDKN2A was significantly correlated with the T stage, N stage and pathological stage of the tumor (P<0.05). ROC curve analysis showed that CDKN2A was more effective in the diagnosis of colon cancer, and the area under the curve was 0.918. CDKN2A and immune checkpoint co-expression showed that CDKN2A was positively correlated with CD274, CTLA4, HAVCR2, LAG3, PDCD1, PDCD1LG2, SIGLEC15 and TIGIT (P<0.05). Spearman correlation analysis showed that CDKN2A expression was positively correlated with CCND1 expression (P<0.05). 【Conclusion】CDKN2A has high efficacy in the diagnosis and prognosis assessment of colon cancer patients, which is a potential target for the treatment of colon cancer, and has important significance in improving the diagnostic accuracy and prognosis assessment of colon cancer.

Key words： Colonic Neoplasms Genes,p16 Databases,Nucleic Acid

收稿日期: 2024-05-13

中图分类号:

R735.35

通讯作者: *E-mail:gsxgongsx@163.com

引用本文:

汪欣宇, 王国清, 龚双喜. 基于TCGA数据库分析CDKN2A基因在结肠癌中的表达及其临床意义[J]. 医学临床研究, 2024, 41(12): 1844-1848.
WANG Xinyu, WANG Guoqing, GONG Shuangxi. The Expression Level and Clinical Value of CDKN2A Gene in Colon Cancer Based on TCGA Database. JOURNAL OF CLINICAL RESEARCH, 2024, 41(12): 1844-1848.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2024.12.006 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2024/V41/I12/1844

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