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Quick Search     Adv Search 2021 Vol. 38, No. 4 Published: 2021-04-26   484 Effects of Aldibenzoquinone Combined with Nierergerine on Oxidative Stress in Patients with Chronic Cerebral Ischemia Dizziness LI Jing, ZHANG Ni DOI: 10.3969/j.issn.1671-7171.2021.04.002 【Objective】 To investigate the effect of aldibenzoquinone combined with Nimergoline on oxidative stress in patients with chronic cerebral ischemia dizziness. 【Methods】 A total of 128 patients with chronic cerebral ischemia dizziness were randomly divided into two groups. The control group was treated with aldibenzoquinone, while the observation group was treated with aldibenzoquinone combined with nimergoline. The levels of inflammatory cytokines (TNF-α, IL-6 and IL-23), never cytokine (BDNF and NGF) in serum and oxidative stress factors (NO, SOD and MDA) before and after treatment were compared between the two groups. 【Results】 There were no significant differences in all factors between the two groups before treatment (P>0.05). After treatment, the clinical efficacy of the observation group was significantly better than that of the control group (P<0.05). The levels of TNF-α, IL-6 and IL-23 inflammatory factors in the observation group were lower than those in the control group (P<0.05). The clinical treatment effect of the observation group was significantly better than that of the control group (P<0.05). The levels of nerve cytokines such as BDNF and NGF in the observation group after treatment were significantly higher than those in the control group (P<0.05). The levels of NO and MDA in the observation group were lower than those in the control group, while the level of SOD was higher than that in the control group (P<0.05). 【Conclusion】 The treatment of chronic cerebral ischemia dizziness patients with aldibenzoquinone combined with nimergoline can improve the level of oxidative stress factor, improve the clinical treatment effect, reduce the level of inflammatory factor and improve the content of nerve cytokines. The clinical effect is satisfactory and it is worthy of application. 2021 Vol. 38 (4): 484-487 [Abstract] ( 62 ) HTML (1 KB)  PDF (1162 KB)  ( 131 ) 488 Effects of Atmospheric Fine Particulate Matter PM2.5 on Necroptosis of Human Microvascular Endothelial Cells HUANG Yi-dan, MA Li, ZHOU Yi-qi, et al DOI: 10.3969/j.issn.1671-7171.2021.04.003 【Objective】 To investigate the effect of atmospheric fine particles (particulate matter) PM2.5 on necroptosis of human vascular endothelial cells.【Methods】 After 24 hours of treatment with a series of concentrations of PM2.5, CCK-8 was used to detect cell viability in HMEC-1 cells (human microvascular endothelial cell line); and the culture supernatant was used to detect the level of lactate dehydrogenase (LDH) produced by HMEC-1 cells. Western blot method was applied to detect the expression and phosphorylation level of necroptosis-related proteins RIPK3 and MLKL. The permeability changes of vascular endothelial cells were measured by trans-well method. After treatment with necrostatin-1, an inhibitor of necroptosis, the effects of PM2.5 on necroptosis, cell damage and permeability of vascular endothelial cells were observed and evaulated. 【Results】 PM2.5 inhibited the proliferation of human microvascular endothelial cells in a dose-dependent manner. It increased the LDH activity in the culture supernatant and upregulated protein expression and phosphorylation levels of RIPK3 and MLKL. The result of trans-well assay showed that PM2.5 increased the permeability of monolayer vascular endothelial cells. The pretreatment of necrostatin-1, an inhibitor of necroptosis, significantly inhibited the protein expression and phosphorylation levels of RIPK3 and MLKL in vascular endothelial cells under PM2.5 stimulation, and reduced the activity of LDH in the supernatant and the permeability of monolayer vascular endothelial cells.【Conclusion】 PM2.5 induces necroptosis of vascular endothelial cells and impairs the integrity of vascular endothelium. 2021 Vol. 38 (4): 488-492 [Abstract] ( 37 ) HTML (1 KB)  PDF (1873 KB)  ( 32 ) 493 Expression and Correlation of NF-κB with Glucocorticoid Receptor of Oral Mucosa in Patients with Oral Lichen Planus YANG Xiu-chuan, LI Fang, SUN Yan DOI: 10.3969/j.issn.1671-7171.2021.04.004 【Objective】 To study the expression and correlation of nuclear factor-κ B (NF-κ b) with glucocorticoid receptor (GR) of oral mucosa in patients with oral lichen planus (OLP). 【Methods】 A total of 126 patients with oral lichen planus who were treated in Department of Stomatology, Anting Hospital,Shanghai Jiading District from February 2017 to February 2019 were selected as clinical research objects (the observation group) for retrospective analysis. And 60 healthy oral mucosa extracted from the oral mucosa, which required to be removed for extraction of impacted teeth in outpatient department, were selected as the control group. The expression of the subunits of NF-κ b including P50 and p65, and the subtypes of GR including GR β (glucocorticoid receptor β) and GR α (glucocorticoid receptor α) in the mucosa of the two groups were analyzed by RT-PCR and Western blotting, and the correlation between NF-κ B and GR in OLP was explored. 【Results】 There were lesions in gingiva, tongue, buccal and other areas in the observation group. The main types of lesions were erosive and mottled. The main pathological manifestations were: banded infiltration of lymphocytes in lamina propria, disorder of basal cell arrangement, irregular prolongation of epithelial nail process, acanthosis, epithelial hyperkeratosis. The expressions of NF-κ B P50, p65, GR β and GR α were shown in the oral mucosa of the two groups. Among them, the expression of GR α protein in the observation group decreased, while the expression of P50 and p65 increased. However, there was no significant change in GR β. The expression level of GR α mRNA in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05); the expression level of P50 and p65 in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05); there was no significant difference in GR β mRNA level between the two groups, the difference was not statistically significant (P>0.05). In the mucosa of patients with oral lichen planus, the expression of P50 was negatively correlated with GR α (r=-0.536,P=0.002). There was also a negative correlation between p65 and GR α (r=-0.428,P=0.015). 【Conclusion】 Under the influence of the clinical treatment effect of glucocorticoids and the chronic inflammation of oral lichen planus, the GR α level of the patients with this disease is down-regulated. NF-κ B and GR have antagonistic effect in the inflammatory response of this disease. 2021 Vol. 38 (4): 493-496 [Abstract] ( 33 ) HTML (1 KB)  PDF (1457 KB)  ( 63 ) 497 Application Value of Ultrasound Combined with MRI in Diagnosis of Neonatal Intracranial Hemorrhage MIAO Yu-tian, KUANG Hai-yan, LUO Ying-chun, et al DOI: 10.3969/j.issn.1671-7171.2021.04.005 【Objective】 To explore the application value of ultrasound combined with craniocerebral MRI(Magnetic Resonance Imaging) in the diagnosis of neonatal intracranial hemorrhagic disease. 【Methods】 The clinical data of 100 children with intracranial hemorrhage admitted to our hospital were retrospectively analyzed. All cases underwent color Doppler ultrasound and cranial MRI examination. The diagnostic accuracy of single ultrasound or MRI was compared to that of ultrasound combined with MRI in the diagnosis of neonatal intracranial hemorrhagic disease. 【Results】 Among 100 children with intracranial hemorrhage, 11 cases were clinically diagnosed with cerebral parenchymal hemorrhage, 13 cases with subarachnoid hemorrhage, 15 cases with subdural hemorrhage, 61 cases with periventricular-intraventricular hemorrhage. The ultrasound detected 9 cases of cerebral parenchymal hemorrhage, 61 cases of periventricular-intraventricular hemorrhage, 3 cases of subdural hemorrhage, 2 cases of subarachnoid hemorrhage; so the diagnosis rate was 75.00% (75/100). MRI diagnosed 11 cases of cerebral parenchymal hemorrhage, 47 cases of periventricular-intraventricular hemorrhage, 13 cases of subarachnoid hemorrhage and 12 cases of subarachnoid hemorrhage. The diagnosis rate with MRI was 83% (83/100). There was no statistically significant difference in the accuracy of ultrasound and MRI in diagnosing intracranial hemorrhage (75.00% vs 83.00%,χ2=2.485,P=0.115). However, the accuracy of ultrasound + MRI in diagnosing intracranial hemorrhage was significantly higher (97.00% vs 75.00% vs 83.00%,χ2=19.451,P<0.001). 【Conclusion】 Both color Doppler ultrasound and MRI can detect and diagnose different types of intracranial hemorrhage. When the two methods are used separately, there is no significant difference in the accuracy of neonatal intracranial hemorrhage diagnosis. Combination of ultrasound and MRI detection can improve the diagnostic accuracy of neonatal intracranial hemorrhagic disease. 2021 Vol. 38 (4): 497-500 [Abstract] ( 57 ) HTML (1 KB)  PDF (2086 KB)  ( 165 ) 501 Effect of Tranexamic Acid in Different Time Windows on the Prognosis of Traumatic Brain Injury NI Yan-qing, GU Yun-lai, FENG Jun-feng DOI: 10.3969/j.issn.1671-7171.2021.04.006 【Objective】 To compare the effects of tranexamic acid on the prognosis of patients with traumatic craniocerebral trauma . 【Methods】 A prospective randomized controlled trial was conducted to analyze the clinical data of 126 patients with traumatic brain injury (GCS ≤ 12), which includes 72 males and 54 females . The patients were divided into Group A (time window < 30 minutes, 46 cases), Group B (time window =1 hour, 42 cases), and Group C (time window =3 hours, 38 cases). Patients of Group A received an intravenous injection of tranexamic acid with a dosage of 1 g at the scene of emergency aid (time window < 30 minutes); patients of Group B took an intravenous injection of tranexamic acid with a dosage of 1 g after admission (time window=1 hour), and patients of Group C took an intravenous injection of 1 g tranexamic acid after a complete examination (time window=3 hours). The operations and other medications were performed according to the routine operations. The average time of ambulance arrivals and the average time of transportation are recorded, and the plasma levels of FDP and D-dimer were observed at the time of admission and 1 day after injury, and a comparison of the times of ICU, DIC score and Glasgow Outcome Score (GOS) at 3 months after injury was also performed. 【Results】 There was no significant difference in the arrival times of ambulance among the three groups. The levels of FDP and D-dimer in the three groups were totally similar (P>0.05). However, 1 day after hospital admission, FDP and D-dimer in the Group A are significantly lower than those in the group B and C; DIC score and NICU time in the Group A were significantly lower than those in the other two groups. The GOS score and prognosis of the Group A were higher in 3 months after injury, and the differences were statistically significant (P<0.05). 【Conclusion】 Tranexamic acid can effectively improve the traumatic coagulation dysfunction, it is available to reduce progressive intracranial hemorrhage with a better prognosis. 2021 Vol. 38 (4): 501-503 [Abstract] ( 27 ) HTML (1 KB)  PDF (1136 KB)  ( 23 ) 504 Comparative Study of TAPP and Traditional Inguinal Hernia Tension-free Repair on Stress and Pain in Adult Inguinal Hernia WANG Wei, ZHAO Yu-bin DOI: 10.3969/j.issn.1671-7171.2021.04.007 【Objective】 To compare the effects of laparoscopic transabdominal preperitoneal prosthesis (TAPP) and traditional inguinal hernia tension-free repair on stress and pain in adult inguinal hernia. 【Methods】 Seventy-four adult patients with inguinal hernia admitted to Our hospital from January 2016 to January 2020 were selected and divided into a control group (January 2016 to January 2018, 37 cases) and TAPP group(From February 2018 to January 2020, 37 cases) according to the operation time . The control group received traditional tension-free inguinal hernia repair, and the TAPP group received TAPP. The operation indexes, complications, pain scores (VAS scores), stress indexes and inflammation indexes were compared between the two groups. 【Results】 The operation time, postoperative bed time, postoperative hospital stay, and gastrointestinal function recovery time of the TAPP group were shorter than those of the control group (all P<0.05), and the intraoperative blood loss was lower than that of the control group (P<0.05), but the cost was higher than the control group (P<0.05). The flap infection or necrosis rate (0.00%) and total complication rate (10.81%) in the TAPP group were lower than those in the control group (0.00%, 35.14%, all P<0.05). The VAS scores of the TAPP group at 6h, 24h and 3 days after operation were lower than those of the control group (all P<0.05). One day after treatment, the MDA, CRP, and PCT in both groups were higher than before treatment (all P<0.05), and SOD was lower than before treatment (all P<0.05). The MDA, CRP, and PCT in the TAPP group were lower than those in the control group (all P<0.05), SOD was higher than the control group (P<0.05). 【Conclusion】 Compared with the traditional tension-free inguinal hernia repair, TAPP has higher safety and quicker postoperative recovery, and has less impact on stress and inflammation. 2021 Vol. 38 (4): 504-507 [Abstract] ( 31 ) HTML (1 KB)  PDF (1167 KB)  ( 86 ) 508 Monitoring and Evaluating Value of Tumor Abnormal Protein Detection in Peripheral Venous Blood of Patients with Breast Cancer SHEN Wei-juan, ZHANG Yi DOI: 10.3969/j.issn.1671-7171.2021.04.008 【Objective】 To explore the efficacy monitoring and evaluation of tumor abnormal protein (TAP) in peripheral venous blood in patients with breast cancer. 【Methods】 A total of 80 cases of female breast cancer patients (the breast cancer group) admitted to the hospital and 68 healthy female volunteers (the control group) who underwent physical examination in our hospital at the same time period were selected for the study. The TAP levels between the breast cancer group and the control group were compared. According to the effect of chemotherapy, breast cancer patients were further divided into the effective group and the ineffective group. The clinical data of effective group and ineffective group were compared. Logistic regression analysis was used to analyze the influencing factors on the therapeutic efficacy of breast cancer patients. Receiver operating characteristic curve (ROC) was used to analyzed the evaluation value of tap in peripheral venous blood for the treatment effect of breast cancer patients. 【Results】 The TAP level of breast cancer group before treatment was higher than that of the control group (P<0.05). The total effective rate of chemotherapy for breast cancer patients was 48.75% (39/80). Compared to the effective group, patients in the ineffective group has higher proportion of clinical staging Ⅲ, poor differentiation, lymph node metastasis, estrogen receptor, progesterone receptor composition ratio and higher TAP level after treatment (P<0.05). The TAP decline rate in the ineffective group was lower than that in the effective group, the difference was statistically significant (P<0.05). Logistic regression analysis results showed that clinical stage, degree of differentiation, lymph node metastasis and TAP decline rate were the influencing factors in the treatment effect of breast cancer patients (P<0.05). ROC analysis showed that the optimal cut-off point for TAP to evaluate the treatment effect of breast cancer patients was 15.59%, with a sensitivity of 73.17%, a specificity of 82.05%, and an AUC value of 0.811. 【Conclusion】 TAP level in the peripheral venous blood is abnormally high in breast cancer patients, and the change of TAP levels before and after treatment is closely related to the treatment effect of breast cancer patients, so it can be used to monitor and evaluate the efficacy of breast cancer patients. 2021 Vol. 38 (4): 508-511 [Abstract] ( 29 ) HTML (1 KB)  PDF (1189 KB)  ( 50 ) 512 Application of Westgard Sigma Rule in Homocysteine Indoor Quality Control LU Sha-sha, DUAN Yong-bo, HAO Xiang-li DOI: 10.3969/j.issn.1671-7171.2021.04.009 【Objective】 To explore the application and evaluation of Westgard sigma rule in the quality control of homocysteine (HCY). 【Methods】 The quality control values of HCY detection from January 2018 to June 2018 (before the use of the Westgard Sigma rule) and July 2018 to December 2018 (after the use of the Westgard Sigma rule) were selected to analyze the out-of-control rate, CV, Bias and the σ values of the two quality control methods. 【Results】 The total out-of-control rate of HCY detection was 2.21% before using Westgard Sigma rule and 1.27% after using HCY rule. The average Bias and CV of Westgard Sigma rule using HCY indoor quality assessment were 2.50% and 7.17%, respectively, and the average Bias and CV after using HCY were 1.82% and 5.66%, respectively. The σ values of low, medium and high HCY indoor quality evaluation before using the Westgard Sigma rule were 7.32, 7.89 and 8.17, which were all greater than 6. The σ values of low, medium and high HCY indoor quality evaluation after using were 5.53, 5.85 and 6.04, that is, the σ values after using the Westgard Sigma rule were lower than before using. 【Conclusion】 Westgard Sigma rule can be used for HCY indoor quality testing and quality improvement, and has clinical application value. 2021 Vol. 38 (4): 512-514 [Abstract] ( 33 ) HTML (1 KB)  PDF (1135 KB)  ( 57 ) 515 Effect of Ultrasound-guided Single Erector Spinalis Plane Block on Analgesia, Inflammatory and Immune Indexes of Pulmonary Lobectomy Under Da Vinci Operation ZHANG Qun-qing, XU Xiang-zhao, LI Jing, et al DOI: 10.3969/j.issn.1671-7171.2021.04.010 【Objective】 To investigate the analgesic effect of single erector spinae plane (ESP) block under ultrasound-guided Da Vinci robotic surgery for patients undergoing lower lobectomy and its effect on inflammatory factors and immune function. 【Methods】 A total of 80 patients who underwent Da Vinci robotic surgery for lower lobectomy was divided into an observation group and a control group, with 40 people in each group by the random number table method. The observation group underwent single ultrasound-guided ESP block before anesthesia induction, and the control group received ultrasound-guided paravertebral nerve block (TPVB) before anesthesia induction.The two groups used patient-controlled intravenous analgesia (PCIA) after operation.The visual simulation method (VAS) was used to evaluate pain score in the rest and cough at the time of extubation (T1), 6h after surgery (T2), 18h after surgery (T3), 24h after surgery (T4) and 48h after surgery (T5).The number of patients getting out of bed cases within 24 hours and the number of PCIA compress-ions within 48 hours after operation were counted.The fasting venous blood of the patients was collected in the morning 24 hours after operation, and the inflammatory factors (IL-6, IL-8, CRP, TNF-α) and immune function indicators (IgG,IgA,IgM) were detected by Enzyme-Linked Immunosorbent Assays (ELISA). 【Results】 Patients in the observation group had lower pain scores in the resting and cough during extubation (T1), 6h (T2), 18h (T3), 24h (T4) and 48h (T5) after surgery(P<0.05).The rate of getting out of bed cases in the observation group was 82.5%, which was higher than that in the control group 62.5%. The difference between the two groups was statistically significant (P<0.05).The number of PCIA compressions in the observation group was lower than that in the control group (P<0.05).The levels of inflammatory factors IL-6, IL-8, CRP, and TNF-α in the observation group were lower than those in the control group (P<0.05).The serum IgG, IgA and IgM immune indexes of observation group were higher than those of control group at 24h after operation (P<0.05). 【Conclusion】 Robot-assisted thoracoscopic lobectomy patients with ultrasound-guided single ESPB have better analgesic effects than TPVB, and postoperative inflammation symptoms are mild, indicating that ESPB has stronger immune function compared with TPVB. 2021 Vol. 38 (4): 515-518 [Abstract] ( 37 ) HTML (1 KB)  PDF (1166 KB)  ( 49 ) 519 Changes of Serum Apelin-12 and AngⅡ in Hypertensive Patients During Induction of General Anesthesia WEN Miao-miao, FAN Jun-bai DOI: 10.3969/j.issn.1671-7171.2021.04.011 【Objective】 To explore the changes of serum Apelin-12 and AngⅡ in hypertensive patients during induction of general anesthesia, to evaluate their vascular protection, and to provide guidance for the patient's postoperative outcome. 【Methods】 From September 2018 to November 2019, 80 patients who underwent laparoscopic gallbladder surgery in the Second Affiliated Hospital of Shanxi Medical University were selected. According to whether they had hypertension, they were divided into hypertension group and control group with 40 cases each. The changes of systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and ST-T of ECG before and after induction of general anesthesia in each group were recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of serum Apelin-12 and angiotensin Ⅱ (Ang Ⅱ) before and after induction of general anesthesia and related analysis. 【Results】 After induction of general anesthesia, serum Apelin-12 decreased and AngⅡ increased in the hypertensive group. The difference was statistically significant (P<0.05), while there was no significant change in the control group (P>0.05). 【Conclusion】 After induction of general anesthesia in hypertensive patients, serum Apelin-12 decreases and AngⅡ increases, suggesting an increased risk of cardiovascular events. Intraoperative precautions should be strengthened to ensure surgical safety. 2021 Vol. 38 (4): 519-521 [Abstract] ( 34 ) HTML (1 KB)  PDF (1137 KB)  ( 17 ) 522 Perioperative Dynamics of cFN, Alb/HbA1c and IFN-γ in Patients with Traumatic Cerebral Hemorrhage undergoing Standard Large Trauma Craniotomy and Relationship with Neurological Function WANG Xiao-li, WANG Yan-yan, YU Yan-hong DOI: 10.3969/j.issn.1671-7171.2021.04.012 【Objective】 To investigate the perioperative dynamics of cellular fibronectin (cFN), albumin (Alb)/glycated hemoglobin (HbA1c) and interferon-γ (IFN-γ) in patients with traumatic cerebral hemorrhage during standard large trauma craniotomy (SLTC) and analyze its relationship with neurological function. 【Methods】 A total of 219 patients with traumatic intracerebral hemorrhage who were scheduled to undergo SLTC surgery between May 2017 and February 2020 in our hospital were selected. According to the prognosis 30 days after operation, they were divided into a good prognosis group (107 cases) and a poor prognosis group (112 cases). The cFN, Alb/HbA1c, IFN-γ, the National Institutes of Health Stroke Scale (NIHSS), and Glasgow Coma Scale (GCS) scores were compared between the two groups. Pearson was used to analyze the correlation of cFN, Alb/HbA1c and IFN-γ with GCS score and NIHSS score. Receiver operating characteristic curve (ROC) and area under ROC (AUC) were used to analyze the efficacy of cFN, Alb/HbA1c, and IFN-γ in predicting prognosis. 【Results】 Compared to those before operation, serum levels of cFN, Alb/HbA1c, IFN-γ, GCS score, and NIHSS score between the two groups of patients at different times were differences; and the differences were statistically significant (P<0.05). If serum levels of cFN, Alb/HbA1c and IFN-γ, GCS score and NIHSS score at 3 and 7 days after operation were compared, there were statistically significant differences between the two groups (P<0.05). The cFN, Alb/HbA1c and IFN-γ at 3 and 7 days after operation were significantly correlated with the GCS score and NIHSS score at 3 and 7 days after operation (P<0.05). The serum cFN, Alb/HbA1c, and IFN-γ levels at 7 d after surgery were more effective in predicting prognosis than at 3 d after surgery. Among them, cFN had the largest AUC for predicting prognosis, which is 0.857.【Conclusion】 In SLTC patients with traumatic cerebral hemorrhage, cFN and IFN-γ tend to increase in the early postoperative period, while Alb/HbA1c tends to decrease. Detecting the level of each index on 7 days after operation can provide important reference information for clinical prediction of prognosis. 2021 Vol. 38 (4): 522-525 [Abstract] ( 32 ) HTML (1 KB)  PDF (1170 KB)  ( 52 ) 526 Value of Erythrocyte Distribution Width and Serum Calcium Ratio in Evaluating the Condition and Prognosis of Patients with Acute Pancreatitis YIN Yang, ZHANG Rui DOI: 10.3969/j.issn.1671-7171.2021.04.013 【Objective】 To investigate the value of erythrocyte distribution width (RDW) and ratio of RDW/serum calcium in evaluating the condition and prognosis of patients with acute pancreatitis. 【Methods】 A total of 153 patients with AP in our hospital were selected as the research subjects. The clinical data of all patients were analyzed and the risk factors affecting the severity and poor prognosis of AP patients were analyzed. The value of RDW and ratio of RDW/serum calcium in evaluating the condition and prognosis of AP patients was explored. 【Results】 Univariate and logistic regression analysis showed that the AP patients with RDW>15.56%, BUN>23.52 mg/dL and ratio of RDW / serum calcium>1.79 were more severe. The AUC of AP patients diagnosed by the ratio of RDW / serum calcium was greater than that of AP patients diagnosed by RDW and BUN, but the difference was not statistically significant (P>0.05).Univariate and logistic regression analysis also showed that the prognosis of AP patients with RDW>15.56%, APACHE Ⅱ>14.06, lactate >2.40mg/dL and ratio of RDW / serum calcium>1.79 was worse. The prognosis of AUC in patients with AP diagnosed by RDW/serum calcium was lower than that of APACHE Ⅱ, while it was higher than that of RDW and lactic acid, but the differences were not statistically significant (P>0.05). 【Conclusion】 RDW/ serum calcium is an independent influence factor of AP patients' worsening condition and poor prognosis. RDW/ serum calcium can be used as a reference index for evaluating the condition and prognosis of AP patients. 2021 Vol. 38 (4): 526-529 [Abstract] ( 34 ) HTML (1 KB)  PDF (1435 KB)  ( 26 ) 530 Changes of Cardiopulmonary Function in AECOPD Patients with Right Ventricular Dysfunction Before and After 6-minute Walking Test Hasiyeti·Tuerxun, XIAO Chun-hong, Aierxiding·Wusiman, et al DOI: 10.3969/j.issn.1671-7171.2021.04.014 【Objective】 To investigate the changes of cardiopulmonary function before and after 6 minutes walking test(6MWT) in patients with right heart dysfunction in acute exacerbation stage of chronic obstructive pulmonary disease. 【Methods】 60 patients with AECOPD were selected from January to December 2019 in the emergency department of our hospital, including 30 patients with right heart dysfunction (observation group) and 30 patients without right cardiac insufficiency (control group). The patients in both groups were treated with 6MWT, and the heart rate (HR), blood pressure (SBP), diastolic pressure (DBP), oxygen saturation (SpO2) were compared before and after 6MWT.【Results】 The 6 minutes walking distance(6MWD) of the observation group was (262.57 ± 83.74) m, which was significantly lower than that of the control group (313.60±75.33) m (t=-2.48,P=0.016). After 6MWT test, HR, SBP, DBP were significantly higher than before 6MWT test, SpO2 was significantly lower than before 6MWT test, the differences were statistically significant (P<0.05); after 6MWT test, there was no significant difference in HR, DBP, SpO2 between the two groups (P>0.05), SBP in the observation group was significantly higher than that in the control group, the difference was statistically significant (t=2.142,P=0.036). There was no significant difference in the change values (△HR, △SBP, △SpO2, △DBP) before and after 6MWT test between the two groups (P>0.05). 6MWD was positively correlated with PaO2, △ SpO2, FEV1% pre, FEV1, FEV1 / FVC (r=0.263, 0.751, 0.598, 0.336, 0.966, all P<0.05); negatively correlated with △SBP, △HR, PaCO2 (r=0.701, 0.557, 0.313, 0.665, 0.779, all P<0.05); not significantly correlated with △DBP, FVC(r=0.017, r=0.017, all P<0.05).【Conclusion】 In AECOPD patients with right ventricular dysfunction, the decline of cardiopulmonary function is more significant. 6MWT alone may not be able to better distinguish the changes of cardiopulmonary function indexes before and after exercise. 2021 Vol. 38 (4): 530-532 [Abstract] ( 30 ) HTML (1 KB)  PDF (1143 KB)  ( 12 ) 533 Effect of Penehyclidine Hydrochloride Combined with Continuous Renal Replacement Therapy on Patients with Acute Severe Organophosphorus Pesticide Poisoning DUAN Wang-wang, LEI Jie-yu DOI: 10.3969/j.issn.1671-7171.2021.04.015 【Objective】 To explore the therapeutic effect of penehyclidine hydrochloride combined with continuous renal replacement therapy (CRRT) in patients with acute and severe organophosphorus pesticide poisoning. 【Methods】 A total of 60 patients with acute severe organophosphorus pesticide poisoning in our hospital were randomly divided into observation group and control group, 30 cases in each group. The control group was treated with penehyclidine hydrochloride, while the observation group was treated with CRRT on the basis of penehyclidine hydrochloride. 【Results】 The survival rate of the observation group (93.33%) was higher than that of the control group (80%) (P>0.05); the recovery time of recovery time and whole blood cholinesterase activity of the observation group were significantly shorter than those of the control group (P<0.05); after treatment, the levels of C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) in the two groups were significantly lower than those at admission (P<0.05), and the levels of CRP and TNF-α in the observation group were significantly lower than those in the control group (P<0.05) 【Conclusion】 Penehyclidine hydrochloride combined with CRRT has good curative effect in the treatment of patients with organophosphorus pesticide poisoning, which can effectively remove the inflammatory factors, shorten the recovery time of patients and the recovery time of whole blood cholinesterase activity, and improve the therapeutic effect. 2021 Vol. 38 (4): 533-535 [Abstract] ( 37 ) HTML (1 KB)  PDF (1139 KB)  ( 30 ) 536 Comparative Study of Clinical Efficacy and the Change of Inflammatory Factors Between the Solitaire Mechanical Thrombectomy and Arterial Thrombolysis in Treatment of Acute Cerebral Infarction LI Tao DOI: 10.3969/j.issn.1671-7171.2021.04.016 【Objective】 To investigate the clinical efficacy and the change of inflammatory factors between the Solitaire mechanical thrombectomy and arterial thrombolvsis in treatment of acute cerebral infarction. 【Methods】 The clinical data of 60 patients with acute cerebral infarction treated in our hospital from June 2017 to July 2019 were analyzed retrospectively. The patients were divided into thrombolysis group (30 patients applied with the intra-arterial thrombolys) and thrombectomy group (30 patients received mechanical thrombectomy) according to the different treatment methods. The NIHSS score after the treatment, cure rate, bleeding rate, the TICI rating to assess the interventional recanalization and the change of inflammatory factors were detected. 【Results】 The NIHSS score of thrombectomy group was better than that of the thrombolysis group in a week after the treatment (P<0.05). The total effective rate reached 70.0% in the mechanical thrombectomy group and 50.0% in the arterial thrombolysis group (P<0.05). The bleeding rate was 6.7% in thrombectomy group, lower than thrombolysis group, the difference was significant (P<0.05). The recanalization rate was lower in thrombectomy group (P<0.05). The value of serum IL-6, TNF-α, hs-CRP at first, seventh and fourteenth day of treatment increased first and decreased afterward, MMP-9 showed a decreasing trend. 【Conclusion】 Compared to thrombolysis, thrombectomy can provide higher effective rate, lower bleeding rate and is beneficial to postoperative recovery. 2021 Vol. 38 (4): 536-538 [Abstract] ( 44 ) HTML (1 KB)  PDF (1137 KB)  ( 76 ) 539 Effect of Various Medial Column Supporting Screws on in the Treatment of Proximal Humeral Fracture with Locking Plate HE Shi-ji, DANG Ya-jun DOI: 10.3969/j.issn.1671-7171.2021.04.017 【Objective】 To investigate the effect of different numbers of medial column supporting screws on proximal humeral fracture with locking plate. 【Methods】 A retrospective analysis of the clinical data of 76 patients with proximal humeral fractures treated in the Orthopedics Department of our hospital from August 2017 to September 2018. They were divided into 4 groups based on the number of medial column support screws used in the surgery: There were 25 patients in the non-supporting screw group, 19 patients in the 1 supporting screw group, 17 patients in the 2 supporting screws group, and 15 patients in the 3 supporting screws group. During the follow-up of 12 months, the shear stiffness, compressive stiffness, fracture healing time, humeral head height loss value, neck shaft angle, varus angle, constant shoulder score and postoperative complications were compared between the four groups. 【Results】 The patients with three supporting screws had the highest shear stiffness and compressive stiffness, which were better than those without supporting screws, one supporting screw group and two supporting screw group (P<0.05). The fracture healing time without supporting screw, 1 supporting screw group, 2 supporting screws group, and 3 supporting screws group were (14.25 ± 2.30), (14.33 ± 2.45), (15.18 ± 3.51)and (15.68 ± 4.63),respectively. There were no statistically significant differences in fracture healing time and neck-shaft angle between the four groups (all P>0.05). The values of humeral head loss and varus angle in group A, group B, group C, and group D decreased sequentially, and the difference between the groups was statistically significant (P<0.05). The Constant scores of shoulder joint function increased gradually in groups A, B, C, and D at 1 month, 2 months, 3 months, 6 months, and 12 months after surgery. The Constant score of group D was the highest, and there were differences between the groups (P<0.05). The incidence of postoperative complications in the 3 supporting screws group, 2 supporting screws group, 1 supporting screw group, and no supporting screw group were 40%(10/25)、42.10% (8/19)、17.64% (3/17) and 13.34% (2/15), respectively. The differences of groups were not statistically significant (P> 0.05). 【Conclusion】 Choosing 2 or 3 screws to support the medial column can achieve a satisfactory recovery results the treatment of proximal humeral fractures with locking plate. The use of 3 supporting screws has the best clinical effect. 2021 Vol. 38 (4): 539-542 [Abstract] ( 29 ) HTML (1 KB)  PDF (1163 KB)  ( 45 ) 543 Significance of Complement-related Factors and Anticardiolipin Antibody Detection in Pregnant Women with Preeclampsia ZHAO Min, HAN Xing DOI: 10.3969/j.issn.1671-7171.2021.04.018 【Objective】 To analyze the levels and significance of maternal complement-related factors and anti-cardiolipin antibodies in preeclampsia. 【Methods】 The clinical data of 66 patients with preeclampsia (preeclampsia group) and 54 patients with severe preeclampsia (severe preeclampsia group) who gave birth in our hospital from December 2016 to March 2018 were selected for retrospective analysis. Thirty-six normal women at the same time were selected as the control group. Placental tissue, serum complement factor D (CFD), adiponectin (ADPN) levels, and serum anticardiolipin antibody (ACA) levels were measured in all maternal women. The expression levels of each factor in the two groups were compared. 【Results】 The levels of CFD in severe preeclampsia were significantly higher than those in the control group and preeclampsia. The ADPN level was significantly lower than the other two groups (P<0.05). The levels of ACA-IgA, ACA-IgG and ACA-IgM in severe preeclampsia were significantly higher than those in the control group. The levels of ACA-IgA and ACA-IgG in severe preeclampsia were significantly higher than those in preeclampsia (P<0.05). 【Conclusion】 Compared with normal pregnant women, the pre-eclampsia maternal body CFD is at a high level, serum ACA-IgA, ACA-IgG and ACA-IgM expression are significantly higher than normal, and is proportional to the severity of the disease, while ADPN is at Low level state, and inversely proportional to the severity of the disease. 2021 Vol. 38 (4): 543-545 [Abstract] ( 41 ) HTML (1 KB)  PDF (1140 KB)  ( 41 ) 546 Effect of Different Gestational Interval on Pregnancy Outcome of Elderly Single Pregnancy YANG Ju-mei, ZHANG Xiao-hua DOI: 10.3969/j.issn.1671-7171.2021.04.019 【Objective】 To explore the effect of different gestational interval on the pregnancy outcome of elderly singleton pregnancy.【Methods】 the clinical data of 880 cases of elderly singleton pregnancy in our hospital from January 2013 to December 2018 were retrospectively analyzed. According to the different delivery interval, they were divided into group A (1-4 years), group B(5-9 years) and group C (≥10 years). The incidence of maternal complications, labor process time and neonatal birth of the three groups were compared. 【Results】 The total incidence of maternal complications in group C was 21.25%(34/160), which was significantly higher than that in group A (14.04%,48/342) and group B (11.11%,42/378), and the difference was statistically significant (P<0.05). But there was no significant difference in the total incidence of maternal complications between group A and group B (P>0.05); the time of the first stage of labor in group A, group B and group C increased in turn, and the difference was statistically significant (P<0.05); The proportion of neonatal asphyxia and transfer to ICU in group C was significantly higher than that in group A and group B (P>0.05), but there was no significant difference between group A and group B (P>0.05). Among 880 cases of single pregnancy, 140 cases had adverse pregnancy outcomes, and the incidence rate was 15.91%. Univariate analysis showed that maternal age, delivery interval, abortion history, adverse pregnancy habits, pregnancy complications and pregnancy complications were related to adverse pregnancy outcome (P<0.05); multivariate logistic regression analysis showed that age ≥ 42 years old, delivery interval ≥ 10 years, abortion history, adverse pregnancy habits, pregnancy complications and pregnancy complications were independent risk factors for adverse pregnancy outcomes in elderly pregnant women (P<0.05). 【Conclusion】 The incidence of complications, the time of the first stage of labor, neonatal asphyxia and the probability of transferring to ICU increases with the extension of gestational interval. 2021 Vol. 38 (4): 546-548 [Abstract] ( 34 ) HTML (1 KB)  PDF (1137 KB)  ( 28 ) 549 Serum Levels Of Lbp and Scd14 in Patients with Primary Sclerosing Cholangitis and Their Relationship with Prognosis LI Zhi-han, ZHANG Xi-liang DOI: 10.3969/j.issn.1671-7171.2021.04.020 【Objective】 To investigate the serum levels of lipopolysaccharide binding protein (LBP) and soluble CD14 (sCD14) in patients with primary sclerosing cholangitis (PSC) and their relationship with prognosis. 【Methods】 87 patients with PSC from January 2008 to January 2014 were selected as the study subjects. The serum levels of LBP and sCD14 were measured by ELISA, and the relationship between them and the prognosis of patients with PSC was analyzed. 【Results】 The incidence of poor prognosis was 40.23% (35/87) in 87 patients with PSC, including 11 cases of liver failure (12.64%), 8 cases of hepatobiliary carcinoma (9.20%), 12 cases of secondary biliary cirrhosis (13.79%) and 4 cases of death (4.60%). The serum levels of LBP and sCD14 in patients with poor prognosis were higher than those in patients with good prognosis (P<0.05). Logistic multivariate regression analysis showed that ALB, TBIL, IL-8, LBP and sCD14 were closely related to the prognosis of PSC patients (P<0.05). The A UC of model C in evaluating the prognosis of PSC patients was higher than that of model A and model B (P<0.05). 【Conclusion】 Serum LBP and sCD14 are correlated with the prognosis of patients with PSC. Detection of serum LBP and sCD14 is helpful to evaluate the prognosis of patients with PSC. 2021 Vol. 38 (4): 549-552 [Abstract] ( 31 ) HTML (1 KB)  PDF (1250 KB)  ( 24 ) 553 Ropivacaine Compounded with Dexmedetomidine Continuous Femoral Nerve Block in Elderly Patients undergoing Total Knee Replacement ZHU Ming-xia, HE Hua-li DOI: 10.3969/j.issn.1671-7171.2021.04.021 【Objective】 To investigate the effect of ropivacaine combined with dexmedetomidine continuous femoral nerve block on analgesia, sleep quality and postoperative delirium in elderly patients with total knee arthroplasty. 【Methods】 A total of 106 elderly patients with total knee replacement who will be treated in our hospital from May 2016 to October 2018 were selected.The patients were randomly divided into control and observation groups by random number table method, with 53 patients in each group.All patients underwent continuous femoral nerve block under ultrasound guidance in both groups, while the control group was administered 0.2% ropivacaine 200ml and the observation group was administered 0.2% ropivacaine 200ml+ 4ug / kg dexmedetomidine.The efficacy of analgesia, quality of sleep, postoperative delirium and occurrence of postoperative complications were compared between the two groups. 【Results】 The numerical rating scale (NRS) and the delirium assessment scale in the ICU (CAM-ICU) scores on postoperative days 1, d2, and d3 were significantly lower in the observation group compared with the control group (P<0.05), and the resting visual analog scale (VAS) scores and motor VAS scores on postoperative days 6, 12, and 24 in the observation group were lower than those in the control group (P<0.05).Satisfaction with analgesia in the observation group was 75.5% (40 / 53) higher than that in the control group at 56.6% (30 / 53) (P<0.05).The number of analgesic pump compressions in the observation group was lower than that in the control group, and the difference was significant (P<0.05).【Conclusion】 Ropivacaine combined with dexmedetomidine continuous femoral nerve block enhances postoperative analgesia, effectively improves sleep quality, and reduces the occurrence of postoperative delirium in elderly patients undergoing total knee replacement. 2021 Vol. 38 (4): 553-555 [Abstract] ( 37 ) HTML (1 KB)  PDF (1139 KB)  ( 33 ) 556 Study on the Association Between HLA-G Gene Polymorphism and Patients with Rheumatoid Arthritis WU Fei, LIU Ting, YAN Xiang-hong DOI: 10.3969/j.issn.1671-7171.2021.04.022 【Objective】 To investigate the association between HLA-G allele polymorphism and the incidence of rheumatoid arthritis (RA) and the production of rheumatoid factor (RF). 【Methods】 through confirmed 95 cases of RA patients and 110 health examiner, to extract all the object of study after peripheral blood, with the method of polymerase chain reaction sequence specific primers (PCR-SSP method) on the research object of HLA-G allele specific amplification and sequencing of the HLA-G type don't statistical analysis, compare the HLA -G allele in the distribution of the frequency of the differences between different groups, and chi-square test for statistical analysis. 【Results】 There was no significant difference in the distribution frequency of four HLA-G alleles between RA group and control group (P>0.05).There was no significant difference in the distribution frequency of four HLA-G alleles between RF positive and RF negative patients in RA group (P>0.05). 【Conclusion】 No difference in the distribution frequency of HLA-G allele was found between RA patients and the normal population, and no protective or susceptible HLA-G allele associated with RA was found. HLA-G may not be directly related to the incidence of RA and the generation of RF. 2021 Vol. 38 (4): 556-558 [Abstract] ( 27 ) HTML (1 KB)  PDF (1143 KB)  ( 34 ) 559 Analysis of Correlation between Carotid Ultrasound Parameters and Left Ventricular Diastolic Function in Patients with Coronary Heart Disease LYU Chun-yan, BIAN Jin-hua DOI: 10.3969/j.issn.1671-7171.2021.04.023 【Objective】 To investigate the relationship of carotid artery ultrasound parameters with left ventricular diastolic function in patients with coronary heart disease.【Methods】 From January 2019 to March 2020, 24 patients with myocardial infarction treated in our hospital were included in the group A, and 30 patients with unstable angina and 26 patients with stable angina treated in the hospital in the same period were enrolled in the group B and group C, respectively. All patients in three groups received carotid ultrasound examination and left ventricular diastolic function measurement. The carotid artery ultrasound parameters [Common carotid artery stiffness (β), carotid artery compliance (AC), elastic coefficient (EP), pulse wave velocity (PEVβ)] and left ventricular diastolic function [Mitral valve E peak/Tissue Doppler E peak (E/Em), Mitral valve E peak/Left ventricular blood flow velocity (E/Vp), Mitral valve E peak/A peak (E/A), pulmonary venous reflux velocity (PVAR), E peak linear velocity time (DT)] of patients in the three groups were compared to analyze the relationship between carotid ultrasound parameters and left ventricular diastolic function. 【Results】 The β, AC, EP, PEVβ, IMT, E/Em, E/A, DT, E/Vp and PVAR values of group A were higher than those of group B and C (P<0.05), while the above parameter values of group B were higher than those of group C (P<0.05).The correlation test results showed that there was a positive correlation between the carotid ultrasound parameters and the levels of left ventricular diastolic function in patients with coronary heart disease (P<0.05). 【Conclusion】 The carotid ultrasound parameters of patients with coronary heart disease are correlated with left ventricular diastolic function. The higher the carotid ultrasound parameters, the worse the left ventricular diastolic function. Clinically, the changes of left ventricular diastolic function in patients with coronary heart disease can be predicted based on the changes of various parameters of the carotid artery, which may be of positive significance for guiding early left ventricular diastolic function evaluation and formulation of treatment plan. 2021 Vol. 38 (4): 559-561 [Abstract] ( 34 ) HTML (1 KB)  PDF (1139 KB)  ( 29 ) 562 Expression and Significance of CTRP6 (C1q/ TNF-Related Protein 6) in Serum of Patients with Type 2 Diabetes Mellitus ZHANG Wen-tao, ZHANG Ni DOI: 10.3969/j.issn.1671-7171.2021.04.024 【Objective】 To investigate the correlation of serum CTRP6 level with insulin resistance in patients with type 2 diabetes. 【Methods】 Ninety patients with type 2 diabetes newly diagnosed in our hospital from June 2017 to April 2019 were selected as the observation group, and 90 healthy patients who underwent physical examination in our hospital during the same period were selected as the control group. General data such as age, gender, height, body mass index (BMI), systolic blood pressure (SBP), and diastolic blood pressure (DBP) of the two groups of candidates were collected.CTRP6, glycated hemoglobin (HbA1c), fasting blood glucose (FPG), 2h postprandial blood glucose (2hPG), fasting insulin (FINS), triglycerides (TG), total cholesterol (TC), and low density lipoprotein ( LDL-C), high-density lipoprotein (HDL-C), interleukin 6 (IL-6), and insulin resistance indicator HOMA-RI.Compare the biochemical index levels of the two groups were mearsured. Taken the CTRP6 as the dependent variable and each index as the independent variable, multiple regression analysis was performed to further analyze the relationship between CTRP6 and each index. 【Results】 The CTRP6 and HDL-C in the observation group were significantly lower than those in the control group (P<0.05). The levels of HOMA-IR, HbA1c, FPG, 2hPG, FINS, TG, TC, LDL-C, and IL-6 in the observation group were significantly higher than those in the control group ( P<0.05).Correlation analysis showed that CTRP6 levels were not significantly related to age, gender, height, SBP, DBP, and TC (P>0.05); CTRP6 levels were negatively related to HOMA-IR, HbA1c, FPG, 2hPG, FINS, TG, LDL-C and IL -6 (P<0.05), and CTRP6 level was positively correlated with HDL-C (P<0.05).Multiple regression analysis was performed using CTRP6 level as the dependent variable and HOMA-IR, HbA1c, FPG, 2hPG, FINS, TG, and BMI as independent variables. The results showed that HOMA-IR, HbA1c, and TG were independent risk factors of CTRP6 (P<0.05). 【Conclusion】 CTRP6 level in patients with type 2 diabetes is significantly lower than normal adults. The level of CTRP6 in patients with type 2 diabetes is closely related to insulin resistance. HOMA-IR, TG, and HbA1c are independent influencing factors of CTRP6. 2021 Vol. 38 (4): 562-565 [Abstract] ( 40 ) HTML (1 KB)  PDF (1162 KB)  ( 203 ) 566 Efficacy and Mechanism of Levetiracetam Combined with Diazepam in Treatment of Children with Febrile Convulsion ZHANG Miao, WANG Yue, ZHANG Peng, et al DOI: 10.3969/j.issn.1671-7171.2021.04.025 【Objective】 To explore the curative effect of levetiracetam combined with diazepam in the treatment of children with febrile convulsion and the effect on serum Zeste gene enhancer homolog 2 (EZH2), neuron-specific enolase (NSE), S100β, neuron-specific growth factor (BDNF) level of influence.【Methods】 A total of 100 children with febrile convulsion admitted to our hospital from January 2018 to December 2019 were selected as the observation group, and 100 children in the same period from outpatient health examination were selected as the healthy control group. According to different treatment methods, the observation group was further divided into the group A (treated with diazepam, n=50) and the group B (treated with levetiracetam combined with diazepam, n=50).Serum EZH2, NSE, S100 β and BDNF levels were recorded and compared. 【Results】 The levels of serum EZH2, NSE, S100β and BDNF in the observation group were higher than those in the healthy control group (P<0.05). The drug onset time and seizure stopping time in the group A were (21.60±3.95) min and (24.51±4.06) min, respectively, while in the group B, they were (17.53±3.04) min and (20.48±3.77) min, respectively. The differences between the two groups were statistically significant (all P<0.05).Before treatment, the levels of serum EZH2, NSE, S100β and BDNF in both group A and B were not statistically significant (P>0.05). After treatment, the levels of serum EZH2, NSE and S100β in the group B were lower than those in the group A (P<0.05). The clinical effective rate of children in group B was 96% (48/50), which was higher than 92% (42/50) of children in group A; and the difference was statistically significant (Z=-2.198, P=0.028<0.05). 【Conclusion】 The serum EZH2, NSE, S100 β,and BDNF levels of febrile convulsion children were significantly higher than those of healthy children. The effect of levetiracetam combined with diazepam on febrile convulsion children was better than that of diazepam alone. 2021 Vol. 38 (4): 566-568 [Abstract] ( 71 ) HTML (1 KB)  PDF (1141 KB)  ( 64 ) 569 Comparision of FCM and Different Types of PCR Techniques in the Monitoring of Small Residual Disease in Children with Acute Lymphocytic Leukemia SONG Na, KUANG Wen-yong DOI: 10.3969/j.issn.1671-7171.2021.04.026 【Objective】 To compare the application of flow cytometry (FCM) and polymerase chain reaction (PCR) in monitoring minimal residual disease (MRD) in children with acute lymphoblastic leukemia. 【Methods】 170 patients diagnosed with acute lymphoblastic leukemia in Hunan children's Hospital from January 2016 to December 2018 were collected, and all of them received therapy with SCCLG-ALL-2016 protocol. All patients were detected immunophenotyping by flow cytometry, and detected fusion genes and immunoglobulin (IG) and T cell receptor (TCR) gene rearrangement by PCR at the initial diagnosis. On the 15th day of treatment, MRD was monitored by FCM and different types of PCR (real-time quantitative PCR,RT-qPCR), multiplex PCR and nested RT-PCR). To analyze the differences of MRD monitoring results between FCM and different types of PCR. 【Results】 The positive rate of MRD detected by FCM was higher than that by RT qPCR and nested RT-PCR, but the differences were not statistically significant(P>0.05). The positive rate of MRD detected by FCM was higher than that by multiplex PCR, and the difference was statistically significant (χ2=34.03,P=0.000). OS of MRD positive patients detected by RT-qPCR, nested RT-PCR and FCM was lower than negative patients, but the difference was not statistically significant (P>0.05). OS of MRD positive patients detected by multiplex PCR was lower than negative patients, and the difference was statistically significant (P=0.002). 【Conclusion】 Technique of FCM and RT-qPCR or nested RT-PCR are both useful to monitor MRD level in pediatric ALL,sensitively.The sensitivity of multiplex PCR in MRD is lower than that of FCM.Multiplex PCR detecting IG or TCR gene rearrangement for MRD monitoring has high specificity but low sensitivity. 2021 Vol. 38 (4): 569-571 [Abstract] ( 43 ) HTML (1 KB)  PDF (1249 KB)  ( 87 ) 572 Effect of Endotracheal Intubation on Oxygen Saturation of Up-Internal Jugular Vein in Patients of Different Ages FEI Jian-ping, ZHAO Ying, JIN Wei-lin, et al DOI: 10.3969/j.issn.1671-7171.2021.04.027 【Objective】 To investigate the effect of endotracheal intubation on cerebral blood flow and cerebral oxygen balance in patients of different ages. 【Methods】 Seventy ASAⅠ-Ⅱpatients were divided into young group (Y group,n42) and old group (O group,n28), The up-internal jugular vein puncture was performed under the guidance of ultrasound at the time of entering the operation groom (T0), 3 min (T1)、 10 min (T2)、 20 min (T3) after tracheal intubation, 20 min (T4) after operation and 20 min (T5) after extubation. The up-internal jugular vein oxygen partial pressure (upPvO2)、 up-internal jugular vein oxygen saturation (upSvO2)、 up-internal jugular vein carbon dioxide partial pressure (upPvCO2)、 up-internal jugular vein lactic acid (upvLac) and up-internal jugular vein glucose (Gluv) were compared. 【Results】 From T0 to T5, the changes of upPvO2 and upSvO2 in the two groups were consistent, After intubation, it was increased fast and then decreased and increased again during operation. Compared with group Y, upPvO2 and upSvO2 increased significantly at T1-T3 in group O (P<0.05), and upPvCO2 increased significantly at T2-T4 in group O (P<0.05). From T0 to T5, upvLac decreased continuously in both groups, Compared with group O, upvLac in group Y decreased significantly at T5 (P<0.05);At T0-T5, the Gluv of group O was higher than that of group Y (P<0.01). 【Conclusion】 The peak value of upPvO2 and upSvO2 after tracheal intubation in elderly patients reflects the rapid increase of cerebral blood flow and cerebral hyperperfusion, which is related to the impairment of cerebrovascular autoregulation in elderly patients。It is particularly important to maintain hemodynamic stability in elderly patients during anesthesia induction. 2021 Vol. 38 (4): 572-575 [Abstract] ( 28 ) HTML (1 KB)  PDF (1166 KB)  ( 53 ) 576 Influence of Acetabular Abduction Angle on Hip Joint Life in Total Hip Arthroplasty YIN Yong, WANG Sheng-tao, ZHANG Bo, et al DOI: 10.3969/j.issn.1671-7171.2021.04.028 【Objective】 To investigate the influence of abduction angle of acetabular prosthesis on hip joint life in total hip arthroplasty (THA). 【Methods】 A total of 347 patients with unilateral THA in our hospital from January 2010 to September 2015 were selected as the research objects. The patients were divided into group A (abduction angle ≥55°) and group B (abduction angle ＜55°) with abduction angle of 55 °as cut-off value. The clinical characteristics of the two groups were observed. The confounding factors were removed by multivariate logistic analysis. The independent correlation between abduction angle and hip joint life span was observed. 【Results】 There were no significant differences in gender, type of hip disease, preoperative activity ability, ASA grade, operation time, replacement site, surgical approach, prosthesis type and postoperative complications in patients with abduction angle ≥55° and <55° respectively (P>0.05), and there were significant differences in age, abduction angle, body mass index and prosthesis survival time between the two groups (P<0.05). Multivariate analysis showed that abduction angle ≥55 ° was a risk factor for hip joint survival. The other influencing factors included age ≥70 years old and body mass index ≥28 kg/m2. 【Conclusion】 The acetabular abduction angle is a risk factor that affects the life of hip prosthesis in THA patients. Clinical methods such as 3D printing assistance and guide positioning can be assisted to improve the positioning accuracy of the acetabular prosthesis, thereby prolonging the patient's hip prosthesis service life. 2021 Vol. 38 (4): 576-578 [Abstract] ( 40 ) HTML (1 KB)  PDF (1136 KB)  ( 62 ) 579 Neuroprotective Effect and Mechanism of NP-PFC Treatment in Rats with Subarachnoid Hemorrhage WANG Chuan-Bao, LIU Jing-jing DOI: 10.3969/j.issn.1671-7171.2021.04.029 【Objective】 To explore the neuroprotective effect and mechanism of nasopharynx perfluorochemical (NP-PFC) treatment in rats with subarachnoid hemorrhage (SAH). 【Methods】 After modeling, forty rats were randomly divided into two groups: model group (group B), NP-PFC treatment group (group C), and another 20 rats were randomly selected as sham operation group (group A). After administration, we evaluated neurological function and morphology of prefrontal cortex (HE staining). The number of apoptotic cells was measured by TUNEL staining. Then, we detected the content of MDA and the activity of SOD and CAT. Finally, the expression of caspase-3 mRNA and caspase-3 protein in brain tissue were detected by PCR and Western Blotting. 【Results】 The neurological function scores of rats in groups A, B, and C were (16.9±2.4), (11.6±2.2), and (15.1±2.4) points, respectively. The neurological function scores of rats in groups B and C were lower than those in group A, while the score in the group C was higher than that in the group B; and the difference was statistically significant (F=26.64, P<0.001). The cell structure of each layer in the prefrontal cortex of the group A rats was in normal pattern, and the edges of nerve cells are clear. The cells of each layer in the prefrontal cortex of group B rats were sparsely arranged, and the edges of nerve cells were fuzzy. When NP-PFC was used, the messy arrangement in the group C was significantly improved compared to the group B, and the edges of nerve cells were clearer than that of group B. Comparison of the contents of MDA, SOD and CAT in the brain tissues of the three groups of rats showed statistically significant differences (F=331.94, 473.27, 275.82, P<0.05). The expression of caspase-3 mRNA in the brain tissues of rats in groups A, B and C were (0.63±0.11), (1.42±0.26), (0.77±0.19), respectively, and the difference was statistically significant (F=46.04, P<0.001). The expression of caspase-3 protein in the brain tissues of rats in groups A, B and C were (0.32±0.08), (0.72±0.13), (0.39±0.09), respectively, and the difference was statistically significant (F=43.60, P< 0.001).【Conclusion】 NP-PFC has neuroprotective effect on the morphology of brain tissue in SAH rats. And it can decrease the quantity of apoptotic cells through oxidative stress inhibition. 2021 Vol. 38 (4): 579-582 [Abstract] ( 27 ) HTML (1 KB)  PDF (1490 KB)  ( 45 ) 583 Effect of Levothyroxine Sodium Combined with Clozapine on Nerve-related Factors in Patients with Schizophrenia LIU Miao, LIU Lei, WANG Sheng-hai DOI: 10.3969/j.issn.1671-7171.2021.04.030 【Objective】 To investigate the effects of levothyroxine sodium combined with clozapine on nerve-related factors (PSA-NCAM: polysialic acid nerve cell adhesion molecules, MBP: myelin basic protein and NSE: neuron specific enolase) in patients with schizophrenia. 【Methods】 A total of 118 patients with schizophrenia in our hospital from January 2018 to October 2019 were selected and divided into two groups with a random number table, with 59 cases in each group. The control group was given clozapine alone, and the observation group was given levothyroxine sodium + clozapine for 6 weeks of continuous treatment. The treatment effects and adverse reactions of the two groups were counted, nerve-related factors (PSA-NCAM, MBP, NSE), thyroid hormone [free triiodothyronine (FT3), free tetraiodothyronine (FT4), thyroid-stimulating hormone (TSH)]. 【Results】 The total effective rate of the observation group was 91.53%, which was higher than that of the control group (76.27%, P<0.05). Serum PSA-NCAM, MBP and NSE in the observation group were lower than those in the control group after 6 weeks of treatment (P<0.05). Serum FT3 and FT4 of the observation group were higher than those of the control group after 6 weeks of treatment (P<0.05). There was no significant difference in serum TSH levels between the two groups after 3 and 6 weeks of treatment (P>0.05). The incidence of dry mouth, constipation, weight gain, tachycardia, and menstrual disorders in the observation group was not statistically different from that in the control group (P>0.05). 【Conclusion】 The combination of levothyroxine sodium and clozapine has a significant effect on the treatment of schizophrenia, which helps to regulate the levels of serum PSA-NCAM, MBP, NSE, FT3, FT4, control agitation behavior, improve sleep quality, improve cognitive function, and has high safety. 2021 Vol. 38 (4): 583-585 [Abstract] ( 40 ) HTML (1 KB)  PDF (1144 KB)  ( 41 ) 586 Relationship between MiR-221-3p and Restenosis after Interventional Therapy for Arteriosclerosis Obliterans of Lower Extremities BAI You-yi DOI: 10.3969/j.issn.1671-7171.2021.04.031 【Objective】 To investigate the effect of MiR-221-3p expression on restenosis among patients with lower extremity arteriosclerosis obliterans (LEASO). 【Methods】 The expression of mir-221-3p in serum was detected by quantitative real-time PCR in 206 patients with leaso from March 2016 to September 2018, and their restenosis was followed up. The working characteristic curve (ROC) of subjects was drawn and the predictive value of MiR-221-3p was evaluated. The risk factors of restenosis were analyzed by multivariate regression. 【Results】 In the follow-up of 6 months after interventional therapy, there were 40 restenosis cases (restenosis group) and 166 non restenosis cases (non restenosis group). The expression level of MiR-221-3p in the restenosis group was higher than that in the non restenosis group, and the proportion of age, BMI, LDL-C, diabetes, hypertension and coronary heart disease in the restenosis group was higher than that in the non restenosis group (P<0.05). The best critical point for MiR-221-3p to predict restenosis was 0.6, and the area under AUC curve was 0.62. The sensitivity and specificity of MiR-221-3p were 52.5% and 71.1%, respectively. Cox multivariate regression analysis showed that older age, obesity, hypertension, diabetes, coronary heart disease and high expression of MiR-221-3p (≥ 0.6) increased the risk of restenosis after interventional therapy (P<0.05).【Conclusion】 The expression level of MiR-221-3p is an independent risk factor for restenosis after interventional therapy in patients with leaso. The risk of restenosis is high in patients with increased expression of MiR-221-3p. 2021 Vol. 38 (4): 586-588 [Abstract] ( 37 ) HTML (1 KB)  PDF (1167 KB)  ( 65 )

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