多重耐药假单胞菌属细菌对碳青霉烯类和氨基糖苷类抗生素耐药的分子机制研究

doi:10.3969/j.issn.1671-7171.2024.02.020

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Abstract 【Objective】 To explore the molecular mechanism of resistance of multidrug-resistant Pseudomonas to carbapenems and aminoglycosides antibiotics. 【Methods】Six strains of multidrug-resistant Pseudomonas aeruginosa and Pseudomonas aeruginosa were received from different wards of our hospital from January 2022 to December in the laboratory department. The bacterial species were identified and verified using the Vitek-2 compact fully automated identification instrument and 16s rDNA sequence primers. The E-test drug sensitivity test was used to determine the antibacterial concentration of the strain against clinical antibiotics. The gene subtypes of aminoglycoside and carbapenemase methylases were identified through PCR amplification and sequence comparison. 【Results】Among the 6 strains of Pseudomonas genus bacteria, 4 strains were Pseudomonas aeruginosa and 2 strains were Pseudomonas putida, both of which were Carba NP positive and produced carbapenemase; The strains showed resistance to penicillin, carbapenems, aminoglycosides, cephalosporins, and tetracycline antibiotics. Among the 6 strains, only SY456 was resistant to aflatoxin with a MIC value of 92 μg/mL, while the other strains were mediators or sensitive to aflatoxin resistance. The Carba NP method screened 6 strains of bacteria, all of which produced drug-resistant B-class carbapenemases. The overlap PCR sequence comparison showed that six strains carried armA gene and blaIMP-45 gene, and three strains of Pseudomonas putida SY47, SY153, and SY434 had significantly different pulse field gel electrophoresis (PFGE) bands. 【Conclusion】 There are both Pseudomonas aeruginosa and Pseudomonas aeruginosa carrying both armA and blaIMP-45 in our hospital. Clinical physicians need to adjust their medication strategies in a timely manner based on the type of drug-resistant strains.

Key words： Pseudomonas aeruginosa Drug Resistance, Bacterial Aminoglycosides Penicillinase

Received: 19 May 2023

PACS:

R378.991

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	WEI Wei
	CHEN Qi

Cite this article:

WEI Wei,CHEN Qi. Molecular Mechanism Study on the Resistance of Multidrug-resistant Pseudomonas to Carbapenems and Aminoglycosides Antibiotics[J]. JOURNAL OF CLINICAL RESEARCH, 2024, 41(2): 230-232.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2024.02.020 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2024/V41/I2/230

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