医学临床研究
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JOURNAL OF CLINICAL RESEARCH  2024, Vol. 41 Issue (2): 230-232    DOI: 10.3969/j.issn.1671-7171.2024.02.020
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Molecular Mechanism Study on the Resistance of Multidrug-resistant Pseudomonas to Carbapenems and Aminoglycosides Antibiotics
WEI Wei, CHEN Qi
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200021
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Abstract  【Objective】 To explore the molecular mechanism of resistance of multidrug-resistant Pseudomonas to carbapenems and aminoglycosides antibiotics. 【Methods】Six strains of multidrug-resistant Pseudomonas aeruginosa and Pseudomonas aeruginosa were received from different wards of our hospital from January 2022 to December in the laboratory department. The bacterial species were identified and verified using the Vitek-2 compact fully automated identification instrument and 16s rDNA sequence primers. The E-test drug sensitivity test was used to determine the antibacterial concentration of the strain against clinical antibiotics. The gene subtypes of aminoglycoside and carbapenemase methylases were identified through PCR amplification and sequence comparison. 【Results】Among the 6 strains of Pseudomonas genus bacteria, 4 strains were Pseudomonas aeruginosa and 2 strains were Pseudomonas putida, both of which were Carba NP positive and produced carbapenemase; The strains showed resistance to penicillin, carbapenems, aminoglycosides, cephalosporins, and tetracycline antibiotics. Among the 6 strains, only SY456 was resistant to aflatoxin with a MIC value of 92 μg/mL, while the other strains were mediators or sensitive to aflatoxin resistance. The Carba NP method screened 6 strains of bacteria, all of which produced drug-resistant B-class carbapenemases. The overlap PCR sequence comparison showed that six strains carried armA gene and blaIMP-45 gene, and three strains of Pseudomonas putida SY47, SY153, and SY434 had significantly different pulse field gel electrophoresis (PFGE) bands. 【Conclusion】 There are both Pseudomonas aeruginosa and Pseudomonas aeruginosa carrying both armA and blaIMP-45 in our hospital. Clinical physicians need to adjust their medication strategies in a timely manner based on the type of drug-resistant strains.
Key wordsPseudomonas aeruginosa      Drug Resistance, Bacterial      Aminoglycosides      Penicillinase     
Received: 19 May 2023     
PACS:  R378.991  
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WEI Wei
CHEN Qi
Cite this article:   
WEI Wei,CHEN Qi. Molecular Mechanism Study on the Resistance of Multidrug-resistant Pseudomonas to Carbapenems and Aminoglycosides Antibiotics[J]. JOURNAL OF CLINICAL RESEARCH, 2024, 41(2): 230-232.
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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2024.02.020     OR     http://journal07.magtech.org.cn/yxlcyj/EN/Y2024/V41/I2/230
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