|
|
|
| Expression Characteristics and Clinical Significance of LAG-3 in CD8+T Cells from Patients with Chronic Hepatitis B Virus Infection |
| ZHANG Chuang, SHEN Hong |
| Department of Respiratory,Xi'an Gaoxin Hospital, Xi'an Shaanxi 710077 |
|
|
|
|
Abstract 【Objective】To investigate the expression characteristics and clinical significance of LAG-3 in CD8+T cells from patients with chronic hepatitis B virus (HBV) infection. 【Methods】A total of 60 patients with chronic HBV admitted to our hospital from April 2019 to April 2020 were selected as the HBV group. While 60 health examinees in our hospital during the same period were selected as the control group. Blood samples were collected from both groups. The expression level, intensity and frequency of LAG-3 in CD8+T cells were measured by flow cytometry. The expression levels of interleukin-10 (IL-10), human leukocyte antigen-DR (HLA-DR), interferon-γ (IFN-γ) and transcription factor T-bet (T-bet) were measured. The mechanism of LAG-3 in regulating CD8+T cells was analyzed. 【Results】The expression level and intensity of LAG-3 in the HBV group were higher than those in the control group (P<0.05).The expression of IL-10 in the LAG-3 positive subgroup was higher than that in LAG-3 negative subgroup within HBV group, and the expression of IL-10 in the LAG-3 negative and positive subgroups within HBV group was higher than that in the control group (P<0.05).The expression of HLA-DR in LAG-3 positive subgroup was lower than that in LAG-3 negative subgroup within HBV group, and the expression of HLA-DR in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group (P<0.05).The expression of IFN-gamma in the LAG-3 positive subgroup within HBV group was lower than that in the LAG-3 negative subgroup, and the expression of IFN-gamma in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group (P<0.05).The expression of T-bet in the LAG-3 positive subgroup of HBV group was lower than that in the LAG-3 negative subgroup, and the expression of IFN-gamma in negative and positive subgroups of HBV group was lower than that in the control group (P<0.05). 【Conclusion】Chronic HBV infection results in the increase of LAG-3 in CD8+T cells under the action of inflammation. LAG-3 can reduce the secretion of IFN-gamma by affecting the expression of IL-10, HLA-DR, IFN-gamma and T-bet, and increase the consumption of T cells.
|
|
Received: 08 December 2022
|
|
|
|
|
|
[1] 胡平平, 李军, 朱传龙. 乙型肝炎病毒体外培养细胞模型研究进展[J].肝脏, 2017, 22(9):841-844.
[2] 马亚丽, 周扬, 平键,等. CD8+CD28-T淋巴细胞与乙型肝炎病毒感染研究进展[J].中华消化杂志, 2017, 37(11):787.
[3] 马晨芸, 陆志成, 王克翔, 等. 慢性乙型肝炎病毒感染患者外周血CD8+ T淋巴细胞表面淋巴细胞活化基因3水平升高[J].细胞与分子免疫学杂志, 2016, 32(4):532-537.
[4] 董月皎, 李雪芬, 崔大伟, 等. 细胞抑制性受体PD-1及LAG-3在慢性乙型肝炎患者CD4+ T细胞表面的表达及其意义[J].临床检验杂志, 2016, 34(2):100-102.
[5] 姚滢, 程婷婷, 黎娜,等. 共抑制分子淋巴细胞活化基因-3在慢性炎症和肿瘤免疫中的作用机制研究进展[J].国际麻醉学与复苏杂志, 2017, 38(3):286-288.
[6] 沈茜, 马晨芸, 陆志成. 淋巴细胞活化基因3的免疫抑制功能及其在慢性病毒感染性疾病中的作用[J].检验医学, 2015, 30(3):298-302.
[7] 陈望, 刘爱玲, 吴敏. 血清IL-10表达水平及其基因多态性与深圳地区慢性乙型肝炎的相关性研究[J].中国优生与遗传杂志, 2018, 26(10):27-29.
[8] 任利, 路越晴, 程冠,等. 青海地区藏族人群IFN-γ、TNF-α及IL-10基因多态性与乙型肝炎病毒感染易感性的关系[J].实用预防医学, 2017, 24(7):814-818.
[9] 张爱芸, 王煜, 马娟,等. 不同临床结局的慢性HBV感染者外周血NK、T细胞比例及IFN-γ、TNF-α水平的意义[J].宁夏医科大学学报, 2018, 40(1):42-46.
[10] HO T H, PFEFFER K, WEISS G J,et al.Identification of a CD4+ T cell line with Treg-like activity[J].Hum Immunol,202,83(4):281-294.
[11] STONE S L, PEEL J N, SCHARER C D,et al.T-bet transcription factor promotes antibody-secreting cell differentiation by limiting the inflammatory effects of IFN-γ on B cells[J].Immunity,2019, 50(5):1172-1187.
[12] 李彤,孙园园.老年CHB患者PD-1、PD-L1表达情况及其与T淋巴细胞的相关性[J].医学临床研究,2023,40(7):995-997.
[13] 童敏.急,慢性乙型肝炎患者外周血中PD-1、T-bet及IFN-γ的水平变化及其意义[J].中国医师杂志, 2020, 22(8):1248-1251. |
|
|
|
2024, Vol. 41 
