Abstract【Objective】 To investigate the effect of etanercept on neuronal apoptosis and the expression of Fas and caspase-8 in diabetes retinopathy (DR) rats. 【Methods】 A total of 30 SPF SD rats were randomly divided into control group, model group and etanercept group with 10 rats in each group. After DR modeling of rats in the etanercept group was successful, 0.4 mg/kg etanercept solution was subcutaneously injected into the front thigh once a day for 8 weeks; The control group and the model group were intraperitoneally injected with 0.01 mol/L citric acid sodium citrate buffer solution of the same pH 4.5 at the same time. After drug intervention, apoptosis of retinal neurons in each group was detected by in situ terminal labeling (TUNEL), and apoptosis index (AI) was calculated; The activity of superoxide dismutase (SOD) and the levels of reactive oxygen species (ROS) and malonaldehyde (MDA) in serum were measured; The levels of Fas and caspase-8 protein in the retina of rats in each group were detected by Western blot. 【Results】 Apoptosis appeared in neurons of the model group and the etanercept group. Compared with the control group, the activity of AI in neuron cells, the activity of SOD in serum, and the levels of Fas and caspase-8 protein in retina of rats in the model group and the etanercept group increased (P<0.05), while the levels of MDA and ROS in serum decreased (P<0.05); The AI of nerve cells, the activity of SOD in serum, the level of Fas and caspase-8 protein in retinal tissue of rats in the etanercept group were lower than those in the model group (P<0.05), and the levels of MDA and ROS in serum were higher than those in the model group (P<0.05). 【Conclusion】 Etanercept may inhibit the activation of Fas/caspase-8 pathway by inhibiting the oxidative stress response of neurons in DR rats, thereby inhibiting neuronal apoptosis.
WANG Kun,ZHANG Li-hua,GAN Xue-feng, et al. Effects of Etanercept on Neuronal Apoptosis and Expression of Fas and Caspase-8 in Rats with Diabetes Retinopathy[J]. JOURNAL OF CLINICAL RESEARCH, 2023, 40(1): 34-36.
2023, Vol. 40 
