|
|
|
| Effects of Etanercept on Neuronal Apoptosis and Expression of Fas and Caspase-8 in Rats with Diabetes Retinopathy |
| WANG Kun, ZHANG Li-hua, GAN Xue-feng,et al |
| Department of Pharmacy,Shaanxi Hospital of Traditional Chinese Medicine,Xi'an Shaanxi 710030 |
|
|
|
|
Abstract 【Objective】 To investigate the effect of etanercept on neuronal apoptosis and the expression of Fas and caspase-8 in diabetes retinopathy (DR) rats. 【Methods】 A total of 30 SPF SD rats were randomly divided into control group, model group and etanercept group with 10 rats in each group. After DR modeling of rats in the etanercept group was successful, 0.4 mg/kg etanercept solution was subcutaneously injected into the front thigh once a day for 8 weeks; The control group and the model group were intraperitoneally injected with 0.01 mol/L citric acid sodium citrate buffer solution of the same pH 4.5 at the same time. After drug intervention, apoptosis of retinal neurons in each group was detected by in situ terminal labeling (TUNEL), and apoptosis index (AI) was calculated; The activity of superoxide dismutase (SOD) and the levels of reactive oxygen species (ROS) and malonaldehyde (MDA) in serum were measured; The levels of Fas and caspase-8 protein in the retina of rats in each group were detected by Western blot. 【Results】 Apoptosis appeared in neurons of the model group and the etanercept group. Compared with the control group, the activity of AI in neuron cells, the activity of SOD in serum, and the levels of Fas and caspase-8 protein in retina of rats in the model group and the etanercept group increased (P<0.05), while the levels of MDA and ROS in serum decreased (P<0.05); The AI of nerve cells, the activity of SOD in serum, the level of Fas and caspase-8 protein in retinal tissue of rats in the etanercept group were lower than those in the model group (P<0.05), and the levels of MDA and ROS in serum were higher than those in the model group (P<0.05). 【Conclusion】 Etanercept may inhibit the activation of Fas/caspase-8 pathway by inhibiting the oxidative stress response of neurons in DR rats, thereby inhibiting neuronal apoptosis.
|
|
Received: 24 November 2021
|
|
|
|
|
|
[1] STRIGLIA E,CACCIOPPO A,CASTELLINO N，et al .Emerging drugs for the treatment of diabetic retinopathy[J]Expert Opin Emerg Drugs ,2020,25(3):261-271.
[2] 刘刚. 王效武,杨纪忠,等.糖尿病视网膜病变患者全视网膜光凝后角膜上皮基底神经丛和朗格汉斯细胞的改变及其相关性[J].中华眼视光学与视觉科学杂志,2021,23(1):34-40.
[3] WANG M,XIONG Y,ZHU W,et al.Sleeve gastrectomy ameliorates diabetes-related spleen damage by improving oxidative stress status in diabetic obese rats[J].Obes Surg,2021,31(3):1183-1195.
[4] 杨爱成,肖炜,魏连波,等.肾康丸对2型糖尿病大鼠TNF-α/IL-6/p-STAT3信号通路的影响[J].新中医,2011,43(9):100-103.
[5] 叶琴,黄焱,朱益华.依那西普对糖尿病大鼠视网膜凋亡通路中Fas、TNF-α及caspase-8表达的影响[J].福建医科大学学报,2019,53(2):29-32.
[6] 杨钦钦,马全鑫,施佳君,等.一种新型糖尿病视网膜病变大鼠模型的建立及其病理特点研究[J].中华糖尿病杂志,2021,13(5):490-497.
[7] 邓小园,陈博,刘红亮,等.TNF-α在丙泊酚诱发的神经元凋亡及认知功能障碍中的作用[J].中国药理学通报,2016,32(7):945-949.
[8] 杨爱成,肖炜,魏连波,等.依那西普靶向治疗影响2型糖尿病大鼠肾脏TRAIL系统表达的观察[J].中国糖尿病杂志,2012,12(6):450-455.
[9] 徐卫星,李勇,徐军.维生素B12对糖尿病大鼠视网膜神经细胞凋亡的影响[J].眼科新进展,2017,37(12):1114-1118.
[10] 赵帆,李佳钰,陆麒瑾,等.当归拈痛汤调控Fas/caspase-8通路促进类风湿关节炎滑膜成纤维细胞凋亡[J].南方医科大学学报,2020,40(8):1119-1126. |
|
|
|
2023, Vol. 40 
