参芪地黄汤对系膜增生性肾小球肾炎大鼠的作用及对p38MAPK/NF-κB信号通路的影响

doi:10.3969/j.issn.1671-7171.2022.08.010

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Abstract 【Objective】To investigate protective effect of Shenqi Dihuang decoction on mesangial proliferative glomerulonephritis (MsPGN) rats and its effect on 38 mitogen-activated protein kinase/nuclear transcription factor Kappa B (p38MAPK/NF-κB) pathway. 【Methods】Forty rats were randomly divided into the sham operation group,MsPGN model group and low and high dose groups of shenqi dihuang decoction,with 10 rats in each group. The rats in the sham-operated group were exposed to the left kidney only,and the rest were the same as those in the model group. The rats in the MsPGN group were exposed to the left kidney only after anesthesia,the rats in the low and high dose groups were given 10 mL/(kg·d) of shenqi dihuang decoction (1.4 mg/mL and 2.8 mg/mL solution) for 4 weeks after the MsPGN model was established,rats in the sham operation group and model group were given the same amount of normal saline at the same time. The 24 h urine protein,urea nitrogen and blood creatinine were measured. The levels of IL-8 and TNF-α in renal tissues were detected by ELISA,and p38MAPK/NF-κB pathway proteins (p38MAPK,p-p38MAPK,NF-κB p65) were detected by Western blotting.【Results】Compared with the sham operation group,the levels of 24 h urinary protein,urea nitrogen,serum creatinine,IL-8,TNF-α,p-p38MAPK/p38MAPK and NF-κB p65 in the model group and Shenqi Dihuang decoction group were all increased (P<0.05). Compared with the model group,the levels of 24 h urinary protein,urea nitrogen,serum creatinine,IL-8,TNF-α,p-p38MAPK/p38MAPK and NF-κB p65 in the low and high dose groups were decreased (P<0.05). Compared with the low-dose group,the levels of all indexes in the high dose group were decreased (P<0.05). 【Conclusion】The protective effect of Shenqi Dihuang decoction on MsPGN rats may be realized by inhibiting the activation of p38MAPK/NF-κB pathway and thus inhibiting the inflammatory reaction of renal tissue.

Key words： Glomerulonephritis, Membranoproliferative/ZD Rats Disease Models, Animal MAP Kinase Signaling System

Received: 17 January 2022

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R692.31

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	WANG Sheng-hui
	ZHAO Qiang

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WANG Sheng-hui,ZHAO Qiang. Protection of Shenqi Dihuang Decoction on Mesangial Proliferative Glomerulonephritis Rats and Its Effect on p38MAPK/NF-κB Signal Pathway[J]. JOURNAL OF CLINICAL RESEARCH, 2022, 39(8): 1153-1156.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2022.08.010 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2022/V39/I8/1153

[1] PAN C,ZHOU C,SHAN A,et al. Expression and clinical significance of thrombospondin-1 and plasminogen activator inhibitor-1 in patients with mesangial proliferative glomerulonephritis[J].Ann Palliat Med,2020,9(5):3187-3193.
[2] WANG L,LIN J,YU T,et al. Identification of plasma miR-106a-5p and miR-30a-5p as potential biomarkers for mesangial proliferative glomerulonephritis[J].Clin Biochem,2020,84(62):79-86.
[3] 戴梅,熊秀萍. 参芪地黄汤辅助治疗IgA肾病的平行随机对照临床研究[J].中医药导报,2016,22(17):83-86.
[4] 南丽红,彭卫华,黄枚,等. 筋骨草总黄酮含药血清对肾小球系膜细胞p38MAPK/NF-κB信号通路的影响[J].中华中医药杂志,2017,32(8):3764-3767.
[5] KUSUNOKI Y,NAMBA-HAMANO T,KAKIMOTO T,et al. A case of proliferative glomerulonephritis with immunoglobulin A1-lambda deposits successfully treated by chemotherapy[J].CEN Case Rep,2020,9(4):326-332.
[6] 陈红波,庄英,马红珍,等. 参芪地黄汤对IgA肾病大鼠肾组织nephrin、podocin蛋白及其mRNA表达的影响[J].中医杂志,2013,69(7):66-69.
[7] 高永红,闫艳. 刘霞教授治疗系膜增生性肾小球肾炎经验的理论初探[J].光明中医,2010,25(11):25-30.
[8] 赵现坤,王飞鹏,丁亚芳. 参芪地黄汤治疗慢性肾衰竭维持性透析合并肾性贫血患者的临床疗效观察[J].健康前沿,2019,28(4):192.
[9] 林素珍,潘晓琼. 参芪地黄汤对慢性肾衰竭患者的治疗效果及机制研究[J].中国中西医结合肾病杂志 2020,21(10):913-914.
[10] SCHNEIDER M F,MUÑOZ A,KU E,et al. Estimation of albumin-creatinine ratio from protein-creatinine ratio in urine of children and adolescents with CKD[J].Am J Kidney Dis,2021,77(5):824-827.
[11] WEI L,FANG Y,CAO G,et al. Genetic and pathological findings in a boy with psoriasis and C3 glomerulonephritis:A case report and literature review[J].Mol Genet Genomic Med,2020,8(10):e1430-e1436.
[12] KIM C S,MATHEW A P,VASUKUTTY A,et al. Glycol chitosan-based tacrolimus-loaded nanomicelle therapy ameliorates lupus nephritis[J].J Nanobiotechnology,2021,19(1):109-205.
[13] LIU D,CHEN C,WANG D,et al. Effect of sericin on the p38MAPK signaling pathway and NLRP3 inflammasome in the kidney of type 2 diabetic rats[J].Exp Ther Med,2020,20(6):267-271.