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| Construction and Evaluation of Diabetes Retinopathy Model |
| LIN Xin-wen, CHEN Na, CHEN Wen-ming, et al |
| Hunan Drug Examination and Inspection Center,Changsha Hunan 410001 |
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Abstract 【Objective】 To establish the model of streptozotocin (STZ)-induced diabetes retinopathy and its evaluation method. 【Methods】 Thirty male SD rats were randomly divided into normal group (10 rats) and model group (20 rats). In the model group, fasting blood glucose was measured 3 days after intraperitoneal injection of STZ (65 mg/kg). Ten diabetes rats with blood glucose >14 mmol/L were selected as the model group. Fasting blood glucose was measured and weighed every 4 weeks. Retinal optical coherence tomography (OCT) was used to observe the changes of retinal blood vessels in diabetes rats, and the morphological changes of retinal tissues in diabetes rats were analyzed by HE staining.【Results】Compared with the normal group, the fasting blood glucose level in the model group increased from 3 days to 20 weeks; OCT imaging results showed that at the 12th week of modeling, the blood vessels in the fundus of the model group were bent and arranged in disorder; At the 20th week of modeling, obvious abnormal neovascularization was observed; The pathological results showed that the retinal nuclear layer of diabetes rats was disorganized from 12 to 20 weeks, the nerve fiber layer and ganglion cell layer were edema, the lumen was dilated, some nuclei were pyknosis, and the number of retinal ganglion cells was significantly reduced.【Conclusion】OCT can be used to dynamically monitor the development of retinopathy and detect fasting blood glucose, which can successfully evaluate the model of diabetes retinopathy.
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Received: 21 July 2022
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2022, Vol. 39 
