呼吸道合胞病毒肺炎患儿Th1、Th2细胞及其分泌的细胞因子与肺功能的相关性分析

doi:10.3969/j.issn.1671-7171.2021.10.026

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摘要【目的】探讨呼吸道合胞病毒(RSV)肺炎患儿辅助性T细胞(Th)1、Th2细胞及其分泌的细胞因子与肺功能的相关性。【方法】选取2017年11月至2020年12月在本院诊治的80例RSV肺炎患儿作为RSV组,另选取52例同期在本院进行健康体检的婴幼儿作为对照组。采用流式细胞技术检测两组外周血中Th1、Th2细胞百分含量,并计算Th1/Th2,采用酶联免疫吸附试验(ELISA)检测Th1、Th2细胞分泌的细胞因子干扰素(IFN)-γ、白细胞介素(IL)-4水平,采用肺功能检测仪检测两组肺功能,比较两组Th1/Th2、血清IFN-γ、IL-4水平及肺功能指标,采用Pearson分析Th1/Th2、血清IFN-γ、IL-4水平与肺功能指标的相关性。【结果】两组性别、年龄、体重指数(BMI)比较差异无统计学意义(P>0.05);RSV组外周血中Th1细胞百分含量、Th1/Th2、IFN-γ显著低于对照组,外周血中Th2细胞百分含量、IL-4显著高于对照组,其差异均有统计学意义(P<0.05);RSV组分钟通气量(MV)、潮气量(VT)、吸气时间(TI)、呼气时间(TE)、呼吸比(TI/TE)、呼气峰流速(PEF)显著低于对照组,呼吸频率(RR)显著高于对照组,其差异均有统计学意义(P<0.05);Th1/Th2与MV、VT、TE、PEF正相关(r=0.86、0.57、0.34、0.67,P<0.05),与RR负相关(r=-0.69,P<0.05);IFN-γ与VT、TI/TE、PEF正相关(r=0.47、0.64、0.70,P<0.05),与RR负相关(r=-0.81,P<0.05);IL-4与MV、VT、TI/TE、PEF负相关(r=-0.47、-0.60、-0.32、-0.63, P<0.05)。【结论】RSV肺炎患儿免疫应答以Th2优势应答为主要表现,Th1、Th2细胞及其分泌的细胞因子与肺功能指标显著相关,可作为病情判断、预后评估的指标。

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关键词 ：呼吸道合胞病毒, 肺炎,病毒性, Th1细胞/免疫学, Th2细胞/免疫学, 呼吸功能试验, 儿童

Abstract：【Objective】To investigate the correlation between helper T cells (Th) 1, Th2 cells, cytokines and lung function in children with respiratory syncytial virus (RSV) pneumonia. 【Methods】A total 80 children with respiratory syncytial virus pneumonia treated in our hospital from November 2017 to December 2020 were selected as RSV group, and 52 infants who underwent physical examination in our hospital at the same time were selected as control group. The percentages of Th1 and Th2 cells in peripheral blood of the two groups were detected by flow cytometry, and Th1/Th2 were calculated; the cytokine interferon (IFN-γ) and interleukin (IL) - 4 levels secreted by Th1 and Th2 cells was detected by enzyme-linked immunosorbent assay (ELISA) ; the pulmonary function of the two groups was detected by pulmonary function tester, and the Th1/Th2 and serum IFN-γ,IL-4 level and pulmonary function index were compared between the two groups; Pearson was used to analyze the correlation between Th1/Th2 ,serum IFN-γ, IL-4 level and pulmonary function indexes. 【Results】 There was no significant difference in gender, age and body mass index (BMI) between the two groups (P>0.05); Percentage of Th1 cells, Th1/Th2 and IFN-γ in peripheral blood of the RSV group. The percentage of Th2 cells and IL-4 in peripheral blood were significantly higher than those in the control group (P<0.05); Minute ventilation (MV), tidal volume (VT), inspiratory time (TI), expiratory time (TE), respiratory ratio (Ti/TE) and peak expiratory flow rate (PEF) in the RSV group were significantly lower than those in the control group, and respiratory rate (RR) was significantly higher than those in the control group (P<0.05); Th1/Th2 was positively correlated with MV, VT, TE and PEF (r=0.86, 0.57, 0.34, 0.67, P<0.05), and negatively correlated with RR (r=-0.69, P<0.05); IFN-γ was positively correlated with VT, Ti/TE and PEF (r=0.47, 0.64, 0.70, P<0.05), and negatively correlated with RR (r=-0.81, P<0.05); IL-4 was negatively correlated with MV, VT, Ti/TE and PEF (r=-0.47, -0.60, -0.32, -0.63, P<0.05). 【Conclusion】 Th2 dominant response is the main manifestation of immune response in children with RSV pneumonia. Th1, Th2 cells and their secreted cytokines are significantly correlated with pulmonary function indexes, which can be used as indexes for judging the condition and evaluating the prognosis.

Key words： Respiratory Syncytial Viruses Pneumonia, Viral Th1 Cells/IM Th2 Cells/IM Respiratory Function Tests Child

收稿日期: 2021-08-20

中图分类号:

R725.631.2

通讯作者: *E-mail:470189815@qq.com

引用本文:

雷蓉, 申洁. 呼吸道合胞病毒肺炎患儿Th1、Th2细胞及其分泌的细胞因子与肺功能的相关性分析[J]. 医学临床研究, 2021, 38(10): 1533-1536.
LEI Rong, SHEN Jie. Correlation between Th1, Th2 Cells, Cytokines and Pulmonary Function in Children with Respiratory Syncytial Virus Pneumonia. JOURNAL OF CLINICAL RESEARCH, 2021, 38(10): 1533-1536.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2021.10.026 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2021/V38/I10/1533

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