长非编码RNA HOTAIR在结直肠癌光动力治疗中的作用机制研究

doi:10.3969/j.issn.1671-7171.2021.10.003

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摘要【目的】探究长链非编码 RNA(LncRNA)-HOX转录反义RNA(HOTAIR)在结直肠癌光动力疗法(PDT)中的作用及机制。【方法】采用实时荧光定量PCR(qPCR)检测PDT处理结直肠癌细胞(CRCs)后LncRNA HOTAIR表达水平的变化。CCK8试剂盒和平板克隆形成实验检测干扰LncRNA HOTAIR表达并用PDT处理后CRCs的增殖情况,同时采用蛋白免疫印迹实验(WB)检测细胞增殖相关蛋白p38以及p-p38的表达情况。然后通过starbase数据库分析LncRNA HOTAIR下游调控蛋白与PDT测序数据进行取交集,得到既是LncRNA HOTAIR的调控蛋白,又在PDT处理后表达下调的蛋白。最后,干扰LncRNA HOTAIR表达的同时过表达ENPP5后采用CCK8试剂盒和平板克隆实验检测细胞增殖水平,采用WB实验检测p38以及p-38蛋白表达。【结果】CRCs经PDT处理后细胞形态发生明显改变,LncRNA HOTAIR表达明显下调。CRCs中干扰LncRNA HOTAIR表达后可提高对PDT的敏感性。CRCs中LncRNA HOTAIR可正向调控ENPP5表达。CRCs中干扰LncRNA HOTAIR表达可以下调ENPP5的表达,影响p38信号通路,促进对PDT的敏感性。【结论】干扰LncRNA HOTAIR表达增加CRCs对PDT的敏感性,LncRNA HOTAIR通过正向调控ENPP5影响PDT对CRCs的敏感性,其为提高结直肠癌PDT疗效提供了新的思路。

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	龚慧
	廖平
	杨庆龙
	唐泽民

关键词 ：结直肠肿瘤/病理学, 光化学疗法, RNA,长链非编码

Abstract：【Objective】To explore the mechanism of long non-coding RNA(LncRNA)-HOX transcribed antisense RNA(HOTAIR) in photodynamic therapy (PDT) of colorectal cancer. 【Methods】The expression of lncrna hotair in colorectal cancer cells (CRCs) treated with PDT was detected by real-time fluorescence quantitative PCR (qPCR). CCK8 kit and plate clone formation experiment were used to detect the proliferation of CRCs after interfering with the expression of LncRNA HOTAIR and treated with PDT. Meanwhile, western blot assay (WB) was used to detect the expression of cell proliferation-related proteins p38 and p-p38.Then, the intersection of LncRNA HOTAIR downstream regulatory protein and PDT sequencing data was analyzed by Starbase database to obtain both the regulatory protein of LncRNA HOTAIR and the down-regulated protein after PDT treatment. Finally, after interfering with the expression of LncRNA HOTAIR and overexpressing ENPP5, the cell proliferation level was detected by CCK8 kit and plate cloning experiment, and the expression of p38 and P-38 protein were detected by WB experiment.【Results】After PDT treatment, the cell morphology of CRCs changed significantly, and the expression of LncRNA HOTAIR was significantly down regulated. Interference with LncRNA HOTAIR expression in CRCs can improve the sensitivity to PDT. LncRNA HOTAIR in CRCs can positively regulate ENPP5 expression. Interfering with the expression of LncRNA HOTAIR in CRCs can down regulate the expression of ENPP5, affect the p38 signal pathway and promote the sensitivity to PDT.【Conclusion】Interference with LncRNA HOTAIR expression increased CRCs sensitivity to PDT, and LncRNA HOTAIR positively regulated ENPP5 to affect PDT sensitivity to CRCs. It provides a new idea for improving the efficacy of PDT in colorectal cancer.

Key words： Colorectal Neoplasms/PA Photochemotherapy RNA,Long Noncoding

收稿日期: 2021-07-21

中图分类号:

R735.37

基金资助:2020年贵州省卫生健康委员会科学技术基因项目[gzwjkj2020-1-100; gzwjkj2020-1-102]

通讯作者: **E-mail:15700718838@163.com

引用本文:

龚慧, 廖平, 杨庆龙, 唐泽民. 长非编码RNA HOTAIR在结直肠癌光动力治疗中的作用机制研究[J]. 医学临床研究, 2021, 38(10): 1448-1452.
GONG Hui, LIAO Ping, YANG Qing-long, et al. Mechanism of LncRNA HOTAIR in Photodynamic Therapy of Colorectal Cancer. JOURNAL OF CLINICAL RESEARCH, 2021, 38(10): 1448-1452.

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http://journal07.magtech.org.cn/yxlcyj/CN/10.3969/j.issn.1671-7171.2021.10.003 或 http://journal07.magtech.org.cn/yxlcyj/CN/Y2021/V38/I10/1448

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