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| Expression, Mutation and Clinical Significance of MUC16 in Non-small Cell Lung Cancer |
| BAI Shan, CAO Cheng |
| Department of Thoracic Surgery, Xi'an XD Group Hospital, Xi'an Shaanxi 710077 |
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Abstract 【Objective】To detect the expression level and mutation of MUC16 in non-small cell lung cancer (NSCLC), and to explore the relationship of expression and mutation of MUC16 with prognosis. 【Methods】A total of 113 patients with NSCLC in our hospital were selected as the research objects. The cancer tissues were in the observation group (A group) and the adjacent "normal" tissues were selected as the control group (B group). Real-time quantitative PCR (qRT-PCR) was used to detect the level of MUC16 mRNA in lung adenocarcinoma and the expression level of MUC16 in lung adenocarcinoma was retrieved from the Ualcan database. The immunohistochemistry was applied to detect the expression level of MUC16 protein. The high throughput sequencing was performed to identify the mutation of MUC16 gene in NSCLC. The diagnostic value of MUC16 mRNA in NSCLC was analyzed and the relationship of prognosis with MUC16 expression level and mutation in patients with NSCLC cancer was analyzed as well. 【Results】In the Ualcan database, the level of MUC16 mRNA in lung adenocarcinoma tissue was shown higher than that in normal lung tissue (P<0.05). The level of MUC16 mRNA and protein positive rate in the A group were higher than those in the B group (P<0.05). The area under the curve (AUC) of MUC16 mRNA level in the diagnosis of NSCLC was 0.882, the cutoff value was 1.213, the specificity was 84.1%, and the sensitivity was 83.2%. The 5-year survival rate of MUC16 positive non-small cell lung cancer patients was 30.14%, which was lower than that of MUC16 negative patients (65.00%, P<0.05). The 5-year survival rate of 22.22% in patients with non MUC16 mutation was lower than that in patients with MUC16 mutation (55.88%, P<0.05). 【Conclusion】MUC16 plays an important role in the occurrence and development of NSCLC cancer, and it has certain diagnostic value. Its expression level and mutation are closely related to the prognosis of NSCLC, which may provide a new potential target for the treatment of NSCLC.
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Received: 05 November 2021
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2022, Vol. 39 
