Abstract【Objective】 The aim of this study is to explore the expression and significance of Cdc42 (cell division cyclin42) in the hippocampus tissue of rats with neurotoxicity of sevoflurane. 【Methods】 Forty-two Sprague Dawley (SD) rats of 7 days old were randomly divided into three groups (n=14): group A, group B and group C. Rats in group A were exposed to 2% sevoflurane and 80% oxygen inhalation once for 2 hours. Rats in group B were exposed to 2% sevoflurane and 80% oxygen inhalation 2 hours/day for three consecutive days. Rats in group C received only 80% oxygen inhalation for 2 hours. Rats were sacrificed at 3 hours after experimental models completed and the hippocampus tissues were collected to measure the expression of Cdc42 and cleaved caspase3 (cysteine proteolytic enzyme 3, CI-Csp3) by western blot. Spatial learning and memory ability of adult rats were detected by Morris water maze (MWM) test at 45-49 days after birth. 【Results】 Compared to the group C, the expression of Cdc42 and Cl-Csp3 in the hippocampus of the group A had no significant difference (P>0.05), whereas the expression of Cdc42 and Cl-Csp3 in the hippocampus of group B increased significantly (P<0.05). In place navigation test, there was no significant difference of the escape latency between group C and group A (P>0.05). The escape latency of rats in the group B were significantly longer than that of C group (P<0.05). In probe trial test, the percent time in target quadrant in group A was not different from that of group C (P>0.05), While the percent time in target quadrant in group B decreased (P<0.05). 【Conclusion】 Multiple inhalation of sevoflurane can induce neurotoxicity and memory dysfunction, which may be related to the increase in the expression of Cdc42.
HAN Tao,CHEN Qin. Expression and Significance of Cdc42 in the Hippocampus Rat Tissue Caused by Sevoflurane Neurotoxicity[J]. JOURNAL OF CLINICAL RESEARCH, 2020, 37(9): 1343-1346.
[1] McCann ME, Bellinger DC, Davidson AJ,et al. Clinical research approaches to studying pediatric anesthetic neurotoxicity[J].Neurotoxicology,2009, 30(5): 766-771. [2] Stratmann G, Sall JW, May LD, et al. Isoflurane differentially affects neurogenesis and long-term neurocognitive function in 60-day-old and 7-day-old rats[J].Anesthesiology,2009, 110(4): 834-848. [3] Shen X, Dong Y, Xu Z, et al. Selective anesthesia-induced neuroinflammation in developing mouse brain and cognitive impairment[J].Anesthesiology,2013, 118(3): 502-515. [4] 马欢欢,张超,郑淑文,等. 新生期大鼠重复吸入七氟烷对其成年期Aβ、Tau蛋白的影响[J].遵义医学院学报,2015,38(7):60-63. [5] 任道全.定位成像诱导激活的Rac1、Cdc42信号传导通路.学位论文.2009. [6] Han T, Hu ZH, Tang YZ, et al. Inhibiting Rho kinase 2 reduces memory dysfunction in adult rats exposed to sevoflurane at postnatal days 7-9[J].Biomed Rep,2015,3(3):361-364. [7] 张洁,欧阳文. 异氟醚对老年大鼠脑组织神经元凋亡的影响[J].中华麻醉学杂志, 2009, 29(7):610-612. [8] Wang WY, Luo Y, Jia LJ, et al. Inhibition of aberrant cyclin-dependent kinase 5 activity attenuates isoflurane neurotoxicity in the developing brain[J].Neuropharmacology,2014, 77: 90-99. [9] Feng X, Liu JJ, Zhou X, et al. Single sevoflurane exposure decreases neuronal nitric oxide synthase levels in the hippocampus of developing rats[J].Br J Anaesth,2012, 109(2): 225-233. [10] Alkire MT, Gruver R, Miller J, et al. Neuroimaging analysis of an anesthetic gas that blocks human emotional memory[J].P Natl Acad Sci USA,2008, 105(5): 1722-1727. [11] Boscolo A, Starr JA, Sanchez V, et al. The abolishment of anesthesia-induced cognitive impairment by timely protection of mitochondria in the developing rat brain: the importance of free oxygen radicals and mitochondrial integrity[J].Neurobiol Dis,2012,45(3):1031-1041. [12] Satomoto M, Satoh Y, Terui K, et al. Neonatal exposure to sevoflurane induces abnormal social behaviors and deficits in fear conditioning in mice[J].Anesthesiology,2009, 110(3): 628-637. [13] Cerione RA, Zheng Y. The Dbl family of oncogenes[J].Curr Opin Cell Biol,1996,8(2):216-222. [14] Suo L, Lu H, Ying G, et al. Protocadherin clusters and cell adhesion kinase regulate dendrite complexity through Rho GTPase[J].J Mol Cell Biol,2012, 4(6): 362-376.
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