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| Effect and Mechanism of Ciprofloxacin (CIP) on Apoptosis of Lung Cancer Cells Induced by TRAIL |
| WANG Bing-chi, WANG Yong-sheng, Gao Pei-hui |
| Department of Respiratory Medicine, Ya'an People's Hospital,Ya'an, Sichuan 625000,China |
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Abstract 【Objective】 To investigate the effect and mechanism of ciprofloxacin (CIP) on apoptosis of lung cancer cells induced by (tumor necrosis factor-associated apoptosis inducing ligand,TRAIL).【Methods】 Human adenocarcinoma A549 cells were divided into blank control group (untreated), CIP group (CIP 100μg/mL in medium), trail group (trail 50 ng/mL in medium) and combination group (CIP 100 μg/mL and trail 50 ng/mL in medium);Trypan blue staining was used to calculate cell viability;The expression of death receptor 4 (DR4), death receptor 5 (DR5) and CCAAT / enhancer binding protein homologous protein (CHOP) were detected by Western blot.【Results】 There was no significant difference between the blank control group and the CIP group in A549 cell viability (P>0.05). The cell viability of the TRAIL group and the CIP group was lower than that of blank the control group and the CIP group (P<0.05), and the A549 cell viability of the combined group was significantly lower than that of the trail group (P<0.05). At 24 h and 48 h, the relative expression levels of CHOP, DR4 and DR5 in the TRAIL group were significantly higher than those in the blank control group and the CIP group (P<0.05), and the relative expression levels of CHOP, DR4 and DR5 in the combined group were significantly higher than those in the TRAIL group (P<0.05). 【Conclusion】 TRAIL combined with CIP can up regulate the expression of DR4 and DR5 in A549 cells, reduce the viability of A549 cells, and increase the apoptosis level of A549 cells; CHOP may play an important role in CIP induced DR5 up regulation and lung adenocarcinoma A549 cell apoptosis, which is worthy of further clinical research
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Received: 02 July 2020
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[1] 熊小玲,刘杰,朱丽萍,等. 2010-2014年江西省肿瘤登记地区肺癌发病率及时间趋势分析[J].中华疾病控制杂志,2017,21(10):1002-1004.
[2] Willms A, Schittek H, Rahn S, et al. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells[J].PLoS One,2019, 14(4):e0214847.
[3] 张述琼,彭世秀. 哌拉西林他唑巴坦龙井分环丙沙星震颤支气管扩张伴铜假单胞菌感染的临床研究[J].现代药物与临床,2018,33(8):1964-1968.
[4] Song JJ, Szczepanski MJ, Kim SY, et al. c-Cbl-mediated degradation of TRAIL receptors is responsible for the development of the early phase of TRAIL resistance[J].Cell Signal,2010, 22(3): 553-563.
[5] Mondal ER, Das SK, Mukherjee P. Comparative evaluation of antiproliferative activity and induction of apoptosis by some fluoroquinolones with a human non-small cell lung cancer cell line in culture[J].Asian Pac J Cancer Prev,2004, 5(2): 196-204.
[6] 王德华,刘云燕,李敏然,等.索拉菲尼联合TRAIL对诱导人肝癌细胞SMMC-7721凋亡的影响[J].解放军医药杂志,2018,30(8):21-24.
[7] Herold C, Ocker M, Ganslmayer M, et al. Ciprofloxacin induces apoptosis and inhibits proliferation of human colorectal carcinoma cells[J].Br J Cancer,2002, 86(3): 443-448.
[8] Phiboonchaiyanan PP, Kiratipaiboon C, Chanvorachote P. Ciprofloxacin mediates cancer stem cell phenotypes in lung cancer cells through caveolin-1-dependent mechanism[J].Chem Biol Interact,2016, 250:1-11.
[9] Capett MS, Vollú-Silva P, Melchiades VA, et al. Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer[J].Curr Microbiol,2016, 73(5):624-632.
[10] Saini S, Sripada L, Tulla K, et al. MADD silencing enhances anti-tumor activity of TRAIL in anaplastic thyroid cancer[J].Endocr Relat Cancer,2019, 26(6):551-563.
[11] Jeon MY, Min KJ, Woo SM, et al. Maritoclax enhances TRAIL-induced apoptosis via chop-mediated upregulation of dr5 and mir-708-mediated downregulation of cFLIP[J].Molecules,2018, 23(11): 3030. |
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2020, Vol. 37 
