|
|
|
| Effect of Human Amniotic Fluid Stem Cell Transplantation on Carbon Tetrachloride Induced Cirrhosis in Rats and its Mechanism |
| LI Sheng, JIAO Zhi-yong, RAO Pei-si, et al |
| Department of Gastroenterology, Yuebei People's Hospital, Shaoguan 512026, China |
|
|
|
|
Abstract 【Objective】To investigate the therapeutic effect and mechanism of human amniotic fluid stem cell transplantation on carbon tetrachloride induced cirrhosis in rats. 【Methods】 Cirrhotic rats were made by injecting 60% carbon tetrachloride vegetable oil subcutaneously for 7 weeks, and then randomly divided into control group (injecting PBS of equal volume),, hAFSC transplantation group and the intervention group of praxafu (AMD3100), a specific blocker of CXCR4 receptor of stromal cell-derived factor-1 (SDF-1) after the transplantation of AFSC.After 3 weeks of treatment, all rats were killed, serum ALP, ALB and CH were measured, and cirrhosis was evaluated according to Ishak scoring standard. In vitro experiments were divided into three groups: control group (PBS), AMD3100 group and AMD3100 + SDF-1 group. The effects of different treatment factors on the survival, migration and adhesion of HFSC were observed. 【Results】Compared with the control group, the serum ALB and Ch in the transplantation group were significantly higher (P<0.05), and ALP had no significant change (P>0.05), while ALP, ALB and Ch in the intervention group were not significantly different from those in the control group (P>0.05). Liver tissue fibrosis scores were statistically significant between the transplant and control groups (P=0.005), and significant differences between the transplant and the intervention groups (P=0.022).The results The results of in vitro experiments showed that SDF-1 could alleviate the inhibition of AMD3100 on the migration, survival and adhesion of hAFSC (P<0.05).【Conclusion】hAFSC transvenous intrahepatic transplantation reduces the extent of carbon tetrachloride-induced cirrhosis in rats and its mechanism is related to SDF-1/CXCR4 regulation.
|
|
Received: 06 November 2019
|
|
|
|
|
|
[1] 陈丽.长期应用人血白蛋白治疗肝硬化腹水疗效的Meta分析[J].医学临床研究, 2018,35(12):2338-2342.
[2] 刘英, 施占立, 赵宗泽, 等. 人脐带源间充质干细胞移植改善四氯化碳诱导肝硬化大鼠的肝纤维化[J].中国组织工程研究, 2012,16(10):1837-1840.
[3] 张博, 刘娜, 刘洪臣. SDF-1/CXCR-4生物轴的研究进展[J].中华老年口腔医学杂志, 2015,13(2):101-104.
[4] Granero Molto F, Weis JA, Miga MI. Regenerative effects of transplanted mesenchymal stem cells in fracture healing[J].Stem Cells,2009,27(8):1887-1898.
[5] Ishak K, Baptista A, Bianchi L, et al. Histological grading and staging of chronic hepatitis.[J].J Hepatol,1995,22(6):696-699.
[6] 高伟, 侯勇. 肝脏硬度值检测诊断慢性乙型肝炎患者肝组织纤维化的效能分析[J].实用肝脏病杂志, 2019,22(5):652-655.
[7] 赵家锋, 焦志勇, 罗敏, 等. CXCR4趋化因子拮抗剂AMD3100对人羊水干细胞抑制作用的体外实验研究[J].临床和实验医学杂志, 2019,18(6):587-589.
[8] 张凯. LP(a)通过下调Akt/eNOS通路损伤内皮祖细胞[D]. 2014.
[9] 倪娟. 肝炎肝硬化患者血清CHE、ALB、CHO指标检测在评估肝功能中的应用价值[J].中国社区医师, 2019,35(19):118.
[10] Shasthry SM, Rastogi A, Bihari C, et al. Histological activity score on baseline liver biopsy can predict non-response to steroids in patients with severe alcoholic hepatitis[J].Virchows Arch,2018,472(4):667-675.
[11] Gazdic M, Volarevic V, Harrell C R, et al. Stem cells therapy for spinal cord injury[J].Int J Mol Sci,2018,19(4):E1039.
[12] Gautam M. Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model[J].J Mol Cell Cardiol,2015,81:139-149.
[13] 焦志勇, 罗敏, 赵家峰, 等. 人羊水干细胞移植治疗肝硬化大鼠的实验研究[J].肝脏, 2013,18(7):457-460.
[14] Ratajczak M Z, Suszynska M. Emerging Strategies to Enhance Homing and Engraftment of Hematopoietic Stem Cells[J].Stem Cell Rev Rep,2016,12(1):121-128.
[15] Mohle R, Bautz F, Rafii S, et al. The chemokine receptor CXCR-4 is expressed on CD34+ hematopoietic progenitors and leukemic cells and mediates transendothelial migration induced by stromal cell-derived factor-1[J].Blood,1998,91(12):4523-4530.
[16] 李盛, 焦志勇, 钟燕明, 等. 趋化因子受体4在人羊水干细胞中的表达水平及其作用[J].吉林医学, 2019,40(12):2755-2757. |
|
|
|
2020, Vol. 37 
