Abstract 【Objective】To investigate the therapeutic effect and mechanism of human amniotic fluid stem cell transplantation on carbon tetrachloride induced cirrhosis in rats. 【Methods】 Cirrhotic rats were made by injecting 60% carbon tetrachloride vegetable oil subcutaneously for 7 weeks, and then randomly divided into control group (injecting PBS of equal volume),, hAFSC transplantation group and the intervention group of praxafu (AMD3100), a specific blocker of CXCR4 receptor of stromal cell-derived factor-1 (SDF-1) after the transplantation of AFSC.After 3 weeks of treatment, all rats were killed, serum ALP, ALB and CH were measured, and cirrhosis was evaluated according to Ishak scoring standard. In vitro experiments were divided into three groups: control group (PBS), AMD3100 group and AMD3100 + SDF-1 group. The effects of different treatment factors on the survival, migration and adhesion of HFSC were observed. 【Results】Compared with the control group, the serum ALB and Ch in the transplantation group were significantly higher (P<0.05), and ALP had no significant change (P>0.05), while ALP, ALB and Ch in the intervention group were not significantly different from those in the control group (P>0.05). Liver tissue fibrosis scores were statistically significant between the transplant and control groups (P=0.005), and significant differences between the transplant and the intervention groups (P=0.022).The results The results of in vitro experiments showed that SDF-1 could alleviate the inhibition of AMD3100 on the migration, survival and adhesion of hAFSC (P<0.05).【Conclusion】hAFSC transvenous intrahepatic transplantation reduces the extent of carbon tetrachloride-induced cirrhosis in rats and its mechanism is related to SDF-1/CXCR4 regulation.
LI Sheng,JIAO Zhi-yong,RAO Pei-si, et al. Effect of Human Amniotic Fluid Stem Cell Transplantation on Carbon Tetrachloride Induced Cirrhosis in Rats and its Mechanism[J]. JOURNAL OF CLINICAL RESEARCH, 2020, 37(3): 329-332.
2020, Vol. 37 
